... amount of carbon and nitrogen, indicating the presence of protein(s). However, the protein wasnot isolated or identified because the focus of thatstudy was identifying the molecular form of the ... [44]. On the other hand, it increases sig-nificantly upon loading of brain mitochondria withCa2+if opening of the PTP is inhibited [44]. There-fore, it is not clear whether opening of the PTP ... single band of approximately 30 kDa on PAGE, and contained sialicacid and neutral and amino sugars, typical of glyco-proteins, a high content of dicarboxylic amino acids, and some bound Ca2+and...