Báo cáo khoa học: Biochemical and structural characterization of mammalian-like purine nucleoside phosphorylase from the Archaeon Pyrococcus furiosus pptx

... here the characterization of the first mammalian-like purine nucleoside phosphorylase from the hyperthermophilic archaeon Pyrococcus furiosus (PfPNP). The gene PF0853 encoding PfPNP was cloned and expressed ... Purification and characterization of extremely thermophilic and thermostable 5 ¢-methylthio- adenosine phosphorylase from the archaeon Sulfo...

Ngày tải lên: 23/03/2014, 09:21

... products of the hazelnut LOX were compared with the reaction products of soybean LOX type I and authentic standards of 9- and 13-HODE. As shown in Fig. 4, 9-HODE is the main product of the hazelnut ... providing the substrates for the activity of all the enzymes located downstream on the pathway, have a primary role in the biosynthesis of phytooxylipins and...

Ngày tải lên: 21/02/2014, 00:20

... only one band was amplified from each of the different samples and Southern blot analysis using the probe prepared with the lac1 primers also revealed a single band. Further- more, all of these generated ... to the culture medium [1]. In this study, we have purified and characterized one of the laccase isoforms, cloned and sequenced the cDNA encoding the enzyme prote...

Ngày tải lên: 07/03/2014, 15:20

... Erlandsen and Stevens [22]. The details of the interactions displayed by residues in the neighborhood of Q301 were analyzed in the structure of the ternary complex of human PAH with BH 4 and ... damage and mental retardation and epilepsy, as well as other neurological abnormalities [3]. The severity of PAH deficiency is variable and partly depends on the natur...

Ngày tải lên: 16/03/2014, 01:20

... describe the further biochemical and spect- roscopic characterization of BphP. Results Expression, purification and initial characterization of recombinant P. aeruginosa phytochrome The P. aeruginosa ... almost all other known members of the classic phytochrome family [12,24]. More recent reports also revealed the presence of the Pfr ground state in Agp2 from A. t...

Ngày tải lên: 16/03/2014, 18:20

... Research, Aliso Viejo, CA) and was found to be similar to the pore size of the membrane used for the extrusion process. The change in the size and poly- dispersity of the liposomes was minimal ... [15]. In this study the protein is natively folded and retains the two glycosyl moieties but is cleaved from the GPI anchor and therefore released from the membra...

Ngày tải lên: 23/03/2014, 10:21

... provides the proton motive force essential for the synthesis of ATP. Homologs of this complex exist in bacteria, the mitochondria of eukaryotes, and the chloroplasts of plants. The bacterial and mitochondrial Keywords chloroplast; ... electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal Biochem 166, 368–379. 42 Laemmli UK (1970) Cle...

Ngày tải lên: 23/03/2014, 13:20

... of the hydrolysis products [12,19]. In order to investigate the effect of bile salts on ChPLA 2 activity, the rate of hydrolysis of PC by ChPLA 2 in the presence of vari- ous concentrations of ... mass of 14 kDa and a specific activity of 400 UÆmg )1 in the presence of 1 mm sodium taur- odeoxycholate and 4 mm CaCl 2 with phosphatidylcholine as substrate. The N...

Ngày tải lên: 30/03/2014, 01:20

... bands of the WT and viridis zb63. The quantification of the bands in the two gradients is presented normalized to the photosystem II (PSII) reaction centre (RC) core band. (C) SDS profile of bands ... solubilization of the viridis zb63 thylakoid membranes and the analyses of one of the resulting sucrose density fractions (band 7) confirmed the pres- ence of th...

Ngày tải lên: 30/03/2014, 10:20

... A3(2). Cloning and expression of the putative hyd genes The deduced open reading frames of putative hyd genes were PCR-amplified from the genomic DNA of the corresponding organisms. The PCR fragments of the ... performed using T-COFFEE [44]. Docking simulation of substrate on the active site of HYD Js The substrate binding pockets of HYD Js and the mode o...

Ngày tải lên: 18/02/2014, 08:20