Báo cáo khoa học: Biochemical and structural characterizatio

Báo cáo khoa học: Biochemical and structural characterization of mammalian-like purine nucleoside phosphorylase from the Archaeon Pyrococcus furiosus pptx

... here the characterization of the ﬁrst mammalian-like purine nucleoside phosphorylase from the hyperthermophilic archaeon Pyrococcus furiosus (PfPNP). The gene PF0853 encoding PfPNP was cloned and expressed ... Puriﬁcation and characterization of extremely thermophilic and thermostable 5 ¢-methylthio- adenosine phosphorylase from the archaeon Sulfo...

Ngày tải lên : 23/03/2014, 09:21

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Tài liệu Báo cáo khoa học: Biochemical and molecular characterization of hazelnut (Corylus avellana) seed lipoxygenases pdf

... products of the hazelnut LOX were compared with the reaction products of soybean LOX type I and authentic standards of 9- and 13-HODE. As shown in Fig. 4, 9-HODE is the main product of the hazelnut ... providing the substrates for the activity of all the enzymes located downstream on the pathway, have a primary role in the biosynthesis of phytooxylipins and...

Ngày tải lên : 21/02/2014, 00:20

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Báo cáo khóa học: Biochemical and molecular characterization of a laccase from the edible straw mushroom, Volvariella volvacea docx

... only one band was ampliﬁed from each of the different samples and Southern blot analysis using the probe prepared with the lac1 primers also revealed a single band. Further- more, all of these generated ... to the culture medium [1]. In this study, we have puriﬁed and characterized one of the laccase isoforms, cloned and sequenced the cDNA encoding the enzyme prote...

Ngày tải lên : 07/03/2014, 15:20

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Báo cáo khoa học: Functional and structural characterization of novel mutations and genotype–phenotype correlation in 51 phenylalanine hydroxylase deﬁcient families from Southern Italy docx

... Erlandsen and Stevens [22]. The details of the interactions displayed by residues in the neighborhood of Q301 were analyzed in the structure of the ternary complex of human PAH with BH 4 and ... damage and mental retardation and epilepsy, as well as other neurological abnormalities [3]. The severity of PAH deﬁciency is variable and partly depends on the natur...

Ngày tải lên : 16/03/2014, 01:20

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Báo cáo khoa học: Biochemical and spectroscopic characterization of the bacterial phytochrome of Pseudomonas aeruginosa pdf

... describe the further biochemical and spectroscopic characterization of BphP. Results Expression, puriﬁcation and initial characterization of recombinant P. aeruginosa phytochrome The P. aeruginosa ... almost all other known members of the classic phytochrome family [12,24]. More recent reports also revealed the presence of the Pfr ground state in Agp2 from A. t...

Ngày tải lên : 16/03/2014, 18:20

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Báo cáo khoa học: Synthesis and structural characterization of a mimetic membrane-anchored prion protein doc

... Research, Aliso Viejo, CA) and was found to be similar to the pore size of the membrane used for the extrusion process. The change in the size and poly- dispersity of the liposomes was minimal ... [15]. In this study the protein is natively folded and retains the two glycosyl moieties but is cleaved from the GPI anchor and therefore released from the membra...

Ngày tải lên : 23/03/2014, 10:21

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Báo cáo khoa học: Isolation and structural characterization of the Ndh complex from mesophyll and bundle sheath chloroplasts of Zea mays pptx

... provides the proton motive force essential for the synthesis of ATP. Homologs of this complex exist in bacteria, the mitochondria of eukaryotes, and the chloroplasts of plants. The bacterial and mitochondrial Keywords chloroplast; ... electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal Biochem 166, 368–379. 42 Laemmli UK (1970) Cle...

Ngày tải lên : 23/03/2014, 13:20

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Báo cáo khoa học: Biochemical and molecular characterization of puriﬁed chicken pancreatic phospholipase A2 docx

... of the hydrolysis products [12,19]. In order to investigate the effect of bile salts on ChPLA 2 activity, the rate of hydrolysis of PC by ChPLA 2 in the presence of vari- ous concentrations of ... mass of 14 kDa and a speciﬁc activity of 400 UÆmg )1 in the presence of 1 mm sodium taur- odeoxycholate and 4 mm CaCl 2 with phosphatidylcholine as substrate. The N...

Ngày tải lên : 30/03/2014, 01:20

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Báo cáo khoa học: Biochemical and structural analyses of a higher plant photosystem II supercomplex of a photosystem I-less mutant of barley pot

... bands of the WT and viridis zb63. The quantification of the bands in the two gradients is presented normalized to the photosystem II (PSII) reaction centre (RC) core band. (C) SDS profile of bands ... solubilization of the viridis zb63 thylakoid membranes and the analyses of one of the resulting sucrose density fractions (band 7) confirmed the presence of th...

Ngày tải lên : 30/03/2014, 10:20

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Tài liệu Báo cáo khoa học: Isolation and molecular characterization of a novel D-hydantoinase from Jannaschia sp. CCS1 docx

... A3(2). Cloning and expression of the putative hyd genes The deduced open reading frames of putative hyd genes were PCR-ampliﬁed from the genomic DNA of the corresponding organisms. The PCR fragments of the ... performed using T-COFFEE [44]. Docking simulation of substrate on the active site of HYD Js The substrate binding pockets of HYD Js and the mode o...

Ngày tải lên : 18/02/2014, 08:20

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