Báo cáo khoa học: Dynamics of a-synuclein aggregation and inhibition of pore-like oligomer development by b-synuclein doc

... results of investigation of chaperone-like activity of MAP2 using dithiothreitol-induced aggregation of insulin at 25 °C. Here, the inhibition of aggregation is about 70% in the presence of 0.1 ... (w/w) of 0.55, 1.1 and 2.2, the inhibitions of insulin aggregation were observed to be 60, 70 and 90%, respectively (curves 2, 3 and 4). With pure tubulin near identic...

Ngày tải lên: 23/03/2014, 12:20

... were separated by SDS/ PAGE and stained by Coomassie brilliant blue. The two hemocyanin bands were cut out and dispersed. About 100 lg of hemocyanin was used for the immunization of rabbits. For ... submitted to MALDI- TOF analysis and assigned to distinct subunits. Fig. 1. SDS/PAGE and immunoblotting of S. coleoptrata hemocyanin. About 10 lg of total hemolymph protein (HL...

Ngày tải lên: 20/02/2014, 11:20

... appear- ance of clouds and the leaf movement by wind. Biotic influences depend mainly on the individual position of a plant within an ecosystem, the density of the plant stand or, in case of algae, ... the surroundings of Jena). The range of photosynthetically active radiation (400–700 nm) and the absorption maxima of PSII and PSI (680 and 700 nm) are marked along the t...

Ngày tải lên: 16/03/2014, 06:20

... preventing the aggregation of luciferase. ClpC1D2 without ATP had no effect on the heat-induced aggregation of luciferase (Fig. 6C). There was some aggregation of ClpC1 and ClpC1D2in the presence of ATP ... prevent protein aggregation and translocate them across cell membranes [3]. Increased expression of heat shock proteins is triggered by a range of stress conditio...

Ngày tải lên: 23/03/2014, 06:20

... obtained from grafit software. Kinetics of reversible inhibition of AspB, T141A and H188A by 3-nitropropionate and D-malate The reversible inhibition of AspB and the T141A and H188A mutants was ... pK a values of pK 1 = 6.8 ± 0.1 and pK 2 = 11.1 ± 0.5 were found. For the H188A mutant, pK a values of pK 1 = 7.4 ± 0.3 and pK 2 = 9.4 ± 0.3 were found. Competitive inh...

Ngày tải lên: 29/03/2014, 23:20

... = y} and F(y) =  ω∈Ω(y) {f ∈ ω}. That is, Ω(y) is the set of parses of a string y and F(y) is the set of features appearing in the parses of y. In the gram- mars of interest here Ω(y) and hence ... also F(y) are finite. Maxwell and Kaplan’s packed representations of- ten provide a more compact representation of the set of parses of a sentence than would be obtained...

Ngày tải lên: 31/03/2014, 06:20

... the amount of IKKb protein in the samples, aliquots of cell lysates were first subjected to West- ern blotting, followed by estimation of the amount of IKKb expressed in each sample by quantitative ... Synergistic activation of NF-jB by LZK and AOP-1 (Eur. J. Biochem. 270)77 The kinase reaction was carried out in kinase reaction buffer containing 0.5 lCi of [c- 32 P] and...

Ngày tải lên: 17/03/2014, 09:20

... Modeling of a-syn oligomer formation FEBS Journal 274 (2007) 1862–1877 ª 2007 The Authors Journal compilation ª 2007 FEBS 1877 Dynamics of a-synuclein aggregation and inhibition of pore-like oligomer ... b- and b-syn, and b- and a-syn heterodimeric interactions. Firstly, theoretical docking of various molecular dynamics conformers of a-syn to conformers of...

Ngày tải lên: 23/03/2014, 09:21

... MHz, and R 2 relaxation delays of 10, 20, 30, 50, 70, 90, 110 and 150 ms were used for data collection at 800 MHz. The errors in R 1 and R 2 were determined by generating random distri- butions of ... variability is reflected by extensive DNA contacts between 9 bp of DNA and the b-ribbon arms, and variable contacts between additional DNA and the body of the protein [19]...

Ngày tải lên: 22/03/2014, 17:20

... kinetics of proton transfers in an individual system is of prime importance in understanding the fine details of the processes involved at the functional and structural levels. However, the dynamics of ... obtained by proton ejection from a photodis- sociable dye. Time-resolved studies of proton transfer for a number of proteins using this method have been reviewed by Gutma...

Ngày tải lên: 22/03/2014, 21:20