Báo cáo khoa học: Cloning, characterization and localization of a novel basic peroxidase gene from Catharanthus roseus potx
Báo cáo khoa học: Cloning, characterization and localization of a novel basic peroxidase gene from Catharanthus roseus potx
... basic
peroxidase gene from Catharanthus roseus
Santosh Kumar, Ajaswrata Dutta, Alok K. Sinha and Jayanti Sen
National Centre for Plant Genome Research, JNU Campus, Aruna Asaf Ali Marg, New Delhi, India
Catharanthus ... database, i.e.
Avicennia (BAB16317), Nicotiana secretory peroxidases (AAD33072), cotton (COTPROXDS) (AAA99868), barley grain (BP1) (AAA32973),
Ar. thaliana (ATP...
Tài liệu Báo cáo khoa học: Cloning, characterization and expression analysis of interleukin-10 from the common carp, Cyprinus carpio L. docx
... GAGGCTAGATACTGCTCGATGT
IL-10 forward3 TGATGATTTGGAACCATTATTGAA
IL-10 reverse3 CACCTTTTTCCTTCATCTTTTCAT
b-Actin forward1 ACTACCTCATGAAGATCCTG
b-Actin reverse1 TTGCTGATCCACATCTGCTG
T7- forward TAATACGACTCACTATAGGG
SP6-reverse ... Cloning, characterization and expression analysis of interleukin-10
from the common carp,
Cyprinus carpio
L.
Ram Savan
1
, Daisuke Igawa
2
and Masahiro Sak...
Báo cáo khoa học: Cloning, expression and interaction of human T-cell receptors with the bacterial superantigen SSA ppt
... 5¢-GGTTATCATA
TAAAGATCTCAAATTACCC-3¢, for the second PCR
the primers were: 5¢ primer, 5¢-GGGTAATTTGAGATC
TTTATATGATAACC-3¢ and 3¢ primer, 5¢-CGCGCGG
GATCCTTAGTGATGGTGATGGTGATGGGTGACC
GGTTTTTTGGTAGGTGAAC-3¢.ThethirdPCRwas
carried ... proliferation assay
We next analyzed the ability of recombinant SSA to
stimulate human T-cells. All SSA preparations yielded
Fig. 1. SDS/PAGE and immunoblot...
Báo cáo khoa học: Identification and localization of glycine in the inner core lipopolysaccharide of Neisseria meningitidis ppt
... data, the majority of structural analyses
on meningococcal core oligosaccharides had been per-
formed following O-deacylation and/ or dephosphorylation.
Naturally, base-labile residues that may ... Identification and localization of glycine in the inner core
lipopolysaccharide of
Neisseria meningitidis
Andrew D. Cox, Jianjun Li and James C. Richards
Institute for Biological Scienc...
Báo cáo khoa học: Purification and properties of a new S-adenosyl-Lmethionine:flavonoid 4¢-O-methyltransferase from carnation (Dianthus caryophyllus L.) pot
... compo-
nents of each reaction mixture, after their chromatographic
separation and purification, were submitted to
1
HNMR
(nuclear magnetic resonance) and FABMS (fast atom
bombardment mass spectrum) analyses ... The
concentration of both the assayed compound and its
respective transformation product was determined by
comparison of peak data with those obtained from
authentic stand...
Báo cáo khoa học: Proper targeting and activity of a nonfunctioning thyroid-stimulating hormone receptor (TSHr) combining an inactivating and activating TSHr mutation in one receptor pptx
... Dickinson and Co.) was used
to acquire and analyze data. A minimum of at least 10 000
cells was analyzed.
Computation of specific constitutive activity (SCA)
and relative SCA (RSCA)
Given that the transfection ... 5-isothiocyanate and
PI. Fluorescence emission of fluorescin 5-isothiocyanate
and PI from single cells were separated and measured using
the standard optics of...
Báo cáo khoa học: Structural features and dynamics of a cold-adapted alkaline phosphatase studied by EPR spectroscopy docx
... The activity in the
standard assay and EPR spectra of the spin-labeled WT AP were
measured after incubation in urea. The scaled mobility factor (M
s
)
was calculated from the central linewidth of ... reduce catalytic activity of a cold-
adapted alkaline phosphatase. Biochim Biophys Acta
1774, 679–687.
32 Janeway CML, Xu X, Murphy JE, Chaidaroglou A &
Kantrowitz ER (1993) Mag...
Báo cáo khoa học: Analyzing the catalytic role of Asp97 in the methionine aminopeptidase from Escherichia coli potx
... 2008)
doi:10.1111/j.1742-4658.2008.06749.x
An active site aspartate residue, Asp97, in the methionine aminopeptidase
(MetAPs) from Escherichia coli (EcMetAP-I) was mutated to alanine, glu-
tamate, and asparagine. Asp97 is the lone carboxylate ... heat of dilution, via an iterative process
using the origin software package. This software pack-
age uses a nonlinear least-square algorith...
Báo cáo khoa học: Cloning, expression and characterization of a new aspartate aminotransferase from Bacillus subtilis B3 docx
... kinetic
parameters K
m
, V
max
and k
cat
were determined for the
purified AATB3. Values for K
m
and V
max
for both
amino donors (l-aspartate and l-glutamate) and ac-
ceptors (a- ketoglutarate and oxaloacetate) ... mML-aspartate
was used as amino donor for a- ketoglutarate, and 30 m
ML-gluta-
mate was used as amino donor for oxaloacetate. The activity of
a- ketoglutarate was adj...
Báo cáo khoa học: Cloning, expression and characterization of a family-74 xyloglucanase from Thermobifida fusca pptx
... purification
The gene for Xeg74 was cloned into pET26b using the
T. fusca Cel 6A signal sequence (MRMSPRPLRALL
GAAAAALVSAAALAFPSQAA) in place of its native
signal sequence. Cel 6A, Cel6Acd, and Cel4 8A have ... by
Chirco & Brown [19] and Jung et al. [12]. Glucose and
xylose oligomer standards were obtained from Seikagaku
America or Sigma.
Preparation of GBG, GBX and tomat...