Báo cáo khoa học: Common mode of DNA binding to cold shock domains Crystal structure of hexathymidine bound to the domain-swapped form of a major cold shock protein from Bacillus caldolyticus pot

... 1279 Common mode of DNA binding to cold shock domains Crystal structure of hexathymidine bound to the domain-swapped form of a major cold shock protein from Bacillus caldolyticus Klaas E. A. Max1, ... Dostal L, Feske A, Max KEA,Welﬂe H, Balbach J & Heinemann U (2004) Single-stranded DNA bound to bacterial cold- shock proteins:preliminary crystallographic and Raman analysis. ActaCrystallogr ... Graumann P, Schnuchel A, Holak TA &Marahiel MA (1995) Mutational analysis of the puta-tive nucleic acid -binding surface of the cold- shock domain, CspB, revealed an essential role of aromaticand...

Báo cáo khoa học: Solution parameters modulating DNA binding speciﬁcity of the restriction endonuclease EcoRV docx

... ﬁnalsample) was used in the self-cleavage assay. (B) The calculatedfraction of total DNA fragment with bound protein as dependent on the total protein added is shown for the gels in (A) . Both the ... f0bÀKnspKspfb1 À fb DNA nsptotal DNA sptotalð1Þwhere [DNA sp]totalis the molar concentration of speciﬁcsequence fragment and [DNA nsp]totalis the molar concen-tration of nonspeciﬁc ... no measurable decrease in protein activity after 18 months storage at )20 °C.Self-cleavage assay To measure the fraction of DNA with speciﬁcally bound EcoRV by the self-cleavage assay, described...

Tài liệu Báo cáo khoa học: High afﬁnity copper binding by steﬁn B (cystatin B) and its role in the inhibition of amyloid ﬁbrillation docx

... (E31 isoform), a variant of the protein (variant 2) with a change from E to Yat amino acid residue 31 (Fig. 1), and a mutant form of the variant (P79S). Copper was found to bind to steﬁn B at pH ... presence of Cu2+(Fig. 5E) may, similarly to P79S (Fig. 4B), arise from enhanced protein aggrega-tion rather than a conformational change. To assess protein stability, thermal denaturation of the protein ... terms of the heat changeper mole of ligand against the ligand to macromoleculeratio. From the binding isotherm values for the reactionstoichiometry, association constants K a , the change inenthalpies...

Báo cáo khoa học: Exo-mode of action of cellobiohydrolase Cel48C from Paenibacillus sp. BP-23 A unique type of cellulase among Bacillales ppt

... the appearance of a terminator was found after the stop codon of cel48C, acting as a signal structure for protein synthesistermination. The protein deduced from cel48C contained 1091 aminoacids ... expres-sion and puriﬁcation. The encoded enzyme, a cellulase of 1091 amino acids with a deduced molecular mass of 118 kDa and a pI of 4.85, displayed a multidomain organ-ization bearing a canonical family ... Exo -mode of action of cellobiohydrolase Cel48C from Paenibacillussp. BP-23 A unique type of cellulase among BacillalesMarta M. Sa´nchez, F. I. Javier Pastor and Pilar DiazDepartment of Microbiology,...

Báo cáo khoa học: "COMMON TOPICS AND COHERENT SITUATIONS: INTERPRETING ELLIPSIS IN THE CONTEXT OF DISCOURSE INFERENCE" ppt

... candidate). The character- istic that Common Topic relations share is that they require the identification of parallel entities (i.e., the al and bi) and relations (P0 and Px) as arguments to ... constituent in the syntax. To summarize this section, we have characterized the forms being addressed according to two features, a sum- mary of which appears in Table 1. Whereas anaphoric Form Empty ... meaning of and conjoining the clauses, and not with its causal asymmetric meaning (para- phraseable by "and as a result"). That is, while either of sentences (1) or (2) can have the...

Tài liệu Báo cáo khoa học: High-afﬁnity ligand binding by wild-type/mutant heteromeric complexes of the mannose pptx

... independently. The tables indicate the amounts of the various cDNAs transfected into cells for each condition andapply to the data shown both above and below the table. Valuesrepresent the mean ± SD of ... format from the amino terminus to the carboxyl terminus, with repeats of the ectodomain shown asrectangles. The shaded rectangles indicate repeats 3 and 9, to which the main determinants of Man-6-P ... if the wild-type and mutant receptorsare binding ligand independently. The tables indicate the amounts of the various cDNAs transfected into cells for each condition andapply to the data shown...

Tài liệu Báo cáo khoa học: Evidence for noncooperative metal binding to the a domain of human metallothionein ppt

... elucidate the potentially cooper-ative nature of the metal -binding reaction within each of the domains. Thus, the goal is to elucidate the meta-llation mechanisms of the individual domains, in the hope, ... the model of a noncooper-ative metallation mechanism. Although a distinctionbetween partially metallated intermediates and the fully metallated holoprotein can be made on the basis of the acquired ... Titration with four molar equivalents of Cd2+was required for the formation of the Cd4 a species in 100% abundance. The implications of a noncooperative metal -binding mechanism are that the partially...

Tài liệu Báo cáo khoa học: Extended half-life upon binding of destabilized intrabodies allows speciﬁc detection of antigen in mammalian cells pdf

... duplexexperiments. The following primers 5¢-GTCTGGCGGAAAACCTCAGTGTGACGC-3¢ and 5¢-GACACCAGACCAACTGGTAATGGTAGCGACCG-3¢ were used for the ampliﬁcation of the 3¢ end of the b-gal gene. The presence of similar amounts ... except that the following primers 5¢-ACTCATACTAGTCTTAGCCATGGCTTCCCGCCG GCG-3¢ and5¢-CCATCCGAATTCTCACTACACATTGAT CCTAGCAGAAGC-3¢ were used for the PCR ampliﬁcation of the C-terminal region of d1-EGFP ... 5¢-CTAGTCGTCCGAACTCCGATAATCGCCGTCAGGGCGGTCGCGAACGTTTAG-3¢ and 5¢-CATGCCAAACGT TCGCGA CCGC CCTGAC GGCGAT TATCGGAGTTCGGACA-3¢ into the unique SpeI restrictionsite. p13R4-DP was obtained by replacing...

Báo cáo khoa học: Nuclear actin and actin-binding proteins in the regulation of transcription and gene expression docx

... MRTFs and SRF activity. Taken together, the small GTPase acts downstream of STARS, and itseems possible that ABLIM integrates signals from the small GTPases, Rac and RhoA (via STARS) toward the actin ... Shijiazhuang, China2 Laboratory of Clinical Investigation, National Institute on Aging, National Institutes of Health, Baltimore, MD, USAActin is a major component of the cytoskeleton andplays ... transcriptioncomplexes.AbbreviationsABLIM, actin -binding LIM protein; ABP, actin -binding protein; ANF, atrial natriuretic factor; AR, androgen receptor; CARM1, coactivator-associated arginine methyltransferase...

Báo cáo khoa học: Chronic high-dose morphine treatment promotes SH-SY5Y cell apoptosis via c-Jun N-terminal kinase-mediated activation of mitochondria-dependent pathway pdf

... release and caspase activation. The Bcl-2family includes antiapoptotic members such as Bcl-2and Bcl-XL, and proapoptotic members such as Bax,Bak, and Bim. Bax and Bak are potent regulators of cytochrome ... the release of cytochrome c and the activation of caspase-9 and caspase-3 in SH-SY5Ycells by western blot analysis. Because activation of caspase-9 and caspase-3 is required for mitochondria-dependent ... apoptosis [33], we ﬁrst detected activation of caspase-9 and caspase-3 upon morphine treatment.Activation of caspases can be manifested either by a reduction in the amount of procaspase (inactive...

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