Báo cáo khoa học: Fast set-up of doxycycline-inducible protein expression in human cell lines with a single plasmid based on Epstein– Barr virus replication and the simple tetracycline repressor ppt
Báo cáo khoa học: Fast set-up of doxycycline-inducible protein expression in human cell lines with a single plasmid based on Epstein– Barr virus replication and the simple tetracycline repressor ppt
... Fast set-up of doxycycline-inducible protein expression in human cell lines with a single plasmid based on Epstein– Barr virus replication and the simple tetracycline repressor Markus Bach1, ... origin of replication forstable and efficient episomal propagation in human cell lines, a cassette forcontinuous expression of the simple tetracycline repressor, and a cytomega-lovirus-type 2 tetracycline ... selection and functionaltesting of individual clones. In contrast, plasmids with an Epstein Barr virus (EBV) origin of replication oriP in the presence of EBV-encoded EBNA-1 protein arecontinuously...
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Báo cáo khoa học: Functional analysis of disease-causing mutations in human galactokinase potx
... effect in human galactokinase. (A) The variation of kcat with mole fraction of deuterium oxide. Thesevalues were obtained in an experiment in which the concentration of ATP was varied and galactose ... galactose was maintained at a saturating level(5 mM). Similar results were obtained when ATP was the saturatingligand and galactose concentration was varied (not shown). (B) The variation in the ... is one cause of the inherited diseasegalactosemia, the main clinical manifestation of which isearly onset cataracts. Human galactokinase (GALK1) wasexpressed in and purified from Escherichia...
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Báo cáo khoa học: Oxygen control of nif gene expression in Klebsiella pneumoniae depends on NifL reduction at the cytoplasmic membrane by electrons derived from the reduced quinone pool doc
... pneumoniae strain contains only a single NADH oxi-dizing enzyme, one can expect a different regulation of the nuo-operon in K. pneumoniae M 5a1 . Based on preliminarysequence analysis of the promoter ... upstream regions of the K. pneumoniae nuoA gene and determination of the NADHoxidation rate in the K. pneumoniae fnr mutant strain, wespeculate that in K. pneumoniae, transcription of the nuo-operon ... nifregulation in K. pneumoniae. K. pneumoniae strain UN4495 was used as the parentalstrain that carries nifLA and a nifK¢-lacZ¢ fusion on the chromosome and thus allows monitoring of NifA-mediatedtranscription...
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Báo cáo khoa học: Functional analysis of disease-causing mutations in human UDP-galactose 4-epimerase pot
... protein and from each other. The main effectseen was in the turnover, kcat, and in the specificityconstant, kcat⁄ Km. Again, this contrasts with disease-causing mutations in galactokinase ... protease inhibi-tors in the solutions used during purification.Kinetic analysis of disease-causing mutations The steady-state kinetic parameters of each of the ninedisease-causing mutant proteins ... digestion have beencompared.Results Expression and purification of human GALE Human GALE was expressed as an N-terminal hexa-histidine fusion protein in E. coli and purified on nickel–agarose resin...
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Báo cáo khóa học: Ouabain stimulates endothelin release and expression in human endothelial cells without inhibiting the sodium pump Robert Saunders and Georgios Scheiner-Bobis pptx
... investigatewhether the observations made thus far are based on a global inhibition of the sodium pump, the uptake of 86Rb+into endothelial cells was determined as a function of the ouabain concentration. ... serum components might induce MAPkinase activation independently of ouabain.Ouabain was added to the cells at various concentra-tions in ECGM-SF, and the cells were further incubatedat 37 °C ... signalsbefore the start of the ouabain treatment. Ouabain wasadded to the cells at various concentrations in fresh ECGM-0.5, and the cells incubated for an additional 12 h. Forcounting, cells were detached...
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Tài liệu Báo cáo khoa học: Crystal structure of Klebsiella sp. ASR1 phytase suggests substrate binding to a preformed active site that meets the requirements of a plant rhizosphere enzyme doc
... consist of two domains, a large a ⁄ bdomain and a small a domain with the catalytic site at the interface of the two domains [4,5]. HAPs can initi-ate hydrolysis of phytate at either the C3(EC ... The kcat⁄ Kmvalues increase from G1P overPhyK to AppA by a factor of 2200. The confor-mational changes of AppA upon substrate bindingfacilitate a faster turnover of phytate and are in ... substrate-freeAppA the C a atoms are 2.41 A ˚apart, whereas for the substrate-free PhyK and the substrate-loaded AppA the averaged distance is only 1.87 A ˚.Distinct conformational changes...
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Tài liệu Báo cáo khoa học: Molecular defect of isovaleryl-CoA dehydrogenase in the skunk mutant of silkworm, Bombyx mori ppt
... concentra-tion was determined using the Coomassie Plus Protein AssayReagent (Pierce) with bovine serum albumin as the standard. Expression and purification of recombinant protein wasconfirmed by SDS ⁄ PAGE ... characterization of IVD deficiency in metazoa.Considering that IVD acts in the third step of leucine degradation and the sku mutant accumulates branched-chain amino acids in haemolymph, thismutant may ... mutant is caused by the accumulation of isovaleric acid in the larval excrement.Second, the mutant exhibits abnormally high accumu-lations of branched-chain amino acids, includingleucine, in...
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Tài liệu Báo cáo khoa học: Molecular determinants of ligand specificity in family 11 carbohydrate binding modules – an NMR, X-ray crystallography and computational chemistry approach doc
... could indicate that the corre-sponding hydroxyl groups are involved in protein binding. The results for the linebroadening measurements of protons H 1a in the alpha and beta configurations,aHa1 and ... com-monly appeared in alternate positions in the carbohy-drate oligomers chain (above and below the plain of the sugar rings) even if the initial calculations wereperformed on a conformation in ... Asp99, Arg126, Asp128 and Asp146 amino acids and, in the case of the largerligands, with Asp51 as well. Most of the hydrogenbonds occur via the hydroxyl groups associated with the C2 and C6 carbon...
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Tài liệu Báo cáo khoa học: Molecular aspects of rheumatoid arthritis: chemokines in the joints of patients pdf
... in am-matory disease that occurs in about 1% of the popula-tion. The in ammatory process is characterized by in ltration of in ammatory cells into the joints, leadingto the proliferation of ... in ltration of in ammatory cellsinto the joints, leading to proliferation of synoviocytes and destruction of cartilage and bone. In RA synovial tissue, the in ltrating cells such asmacrophages, T cells, ... N,Kamogawa J, Yamamoto H, Takubo N, Nakata S,Yamada K, Yamamoto S et al. (2005) Accumulation of plasma cells expressing CXCR3 in the synovial sublin-ing regions of early rheumatoid arthritis in associationwith...
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Tài liệu Báo cáo khoa học: Inhibitory effects of nontoxic protein volvatoxin A1 on pore-forming cardiotoxic protein volvatoxin A2 by interaction with amphipathic a-helix doc
... VVA1 (VVA2beads) was incubated with increasing amounts of VVA2, and visualization on an SDS ⁄ PAGE gel showedthat the adsorbed VVA2 had oligomerized. As the amount of VVA2 in the reaction was ... Characteristics of volvatoxin A1 (VVA1) structure. (A) Alignment of the deduced amino acid sequence of VVA1 N-terminal domain(VVA1-NTD) and VVA1 C-terminal domain (VVA1-CTD) with that of VVA2 ... lanes 1–4), which may imply thatone molecule of VVA2 has one binding site forinteracting with either VVA1 or VVA2. Additionalinvestigation of the characteristics of binding of VVA2to VVA1...
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Từ khóa: Nghiên cứu tổ chức pha chế, đánh giá chất lượng thuốc tiêm truyền trong điều kiện dã ngoạiNghiên cứu tổ hợp chất chỉ điểm sinh học vWF, VCAM 1, MCP 1, d dimer trong chẩn đoán và tiên lượng nhồi máu não cấpBiện pháp quản lý hoạt động dạy hát xoan trong trường trung học cơ sở huyện lâm thao, phú thọGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANQuản lý hoạt động học tập của học sinh theo hướng phát triển kỹ năng học tập hợp tác tại các trường phổ thông dân tộc bán trú huyện ba chẽ, tỉnh quảng ninhNghiên cứu khả năng đo năng lượng điện bằng hệ thu thập dữ liệu 16 kênh DEWE 5000Định tội danh từ thực tiễn huyện Cần Giuộc, tỉnh Long An (Luận văn thạc sĩ)Tìm hiểu công cụ đánh giá hệ thống đảm bảo an toàn hệ thống thông tinThơ nôm tứ tuyệt trào phúng hồ xuân hươngChuong 2 nhận dạng rui roKiểm sát việc giải quyết tố giác, tin báo về tội phạm và kiến nghị khởi tố theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn tỉnh Bình Định (Luận văn thạc sĩ)Tăng trưởng tín dụng hộ sản xuất nông nghiệp tại Ngân hàng Nông nghiệp và Phát triển nông thôn Việt Nam chi nhánh tỉnh Bắc Giang (Luận văn thạc sĩ)Nguyên tắc phân hóa trách nhiệm hình sự đối với người dưới 18 tuổi phạm tội trong pháp luật hình sự Việt Nam (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtTÁI CHẾ NHỰA VÀ QUẢN LÝ CHẤT THẢI Ở HOA KỲQUẢN LÝ VÀ TÁI CHẾ NHỰA Ở HOA KỲ