Báo cáo khoa học: Crystal structure and solution characterization of the activation domain of human methionine synthase doc

... F 0 is the measure of the fluorescence intensity in the absence of the activation domain, E 0 is the concentration of the FMN domain, L denotes the concentration of the activation domain added to the ... (2) the location of the N-terminus in solution is not consistent with models of the dimer (the N-terminus is not visible in the crystal stru...

Ngày tải lên: 23/03/2014, 09:21

... similar domain structure to ChiC, consisting of a catalytic domain and an N-terminal chitin-binding domain. ChiG, on the other hand, lacks this chitin-binding domain, and consists only of a catalytic ... N-terminal extension of eight and seven residues respectively (Fig. 1). The N-termini of the plant enzymes and the first residue in the ChiG structure (Phe1...

Ngày tải lên: 07/03/2014, 11:20

... sites within the two domains. This was followed by coordi- nation of the fourth and fifth metal ions to the a domain for completion of the metallation of this domain prior to filling of the b domain. ... to elucidate the meta- llation mechanisms of the individual domains, in the hope, initially, of simplifying the interpretation of the metallation detail...

Ngày tải lên: 19/02/2014, 00:20

... metal-binding site at the interface of the two domains, which consists of four side chains: two (His31 and His79) from the N-terminal domain and two (Asp165 and His169) from the C-terminal domain (Fig. ... of the reconstituted enzymes were higher (Table 1), the crystallographic studies were per- formed on apo- and metal-reconstituted ApeSODs. Crystallization and...

Ngày tải lên: 14/02/2014, 22:20

... those in the TG structure. The arrow indicates the Tyr27 residue. The mean and SD of the val- ues are 0.25 A ˚ and 0.15 A ˚ , respectively. (B) The backbone atoms of liganded and unliganded structures ... Displacement of the backbone atoms of Mglu and Bacillus glutaminase induced by ligand binding. Displacement val- ues for backbone atoms of the nonliganded...

Ngày tải lên: 06/03/2014, 09:22

... enzy- matic part, and (b) the variability of the angle of approach of the inhibitor relative to the catalytic cleft of the enzyme. The latter factor may reflect not so much the geometry of the catalytic ... view of the interactions of papain with loop L6 of chagasin and loop L2 of cysta- tin B. (C) Zoom-in view of the interactions of papain with lo...

Ngày tải lên: 16/03/2014, 04:20

... their endocytosis [19]. The stoichiometry of RI binding to RATEs and the role of the RI–RATE complex in the in vitro inacti- vation of RATEs have so far been obscure. The crystal structures of three ... relaxed in solution, thereby enabling RI binding to both the N-terminal and the C-terminal RNase A entities. The distance between the C-terminus of the firs...

Ngày tải lên: 22/03/2014, 16:21

... measure- ment of k* on and k* off . At [LCTI] ⁄ [trypsin] ¼ 1.1 and 2.25 the conversion of LCTI into LCTI* (Fig. 3, curves 2 and 3), allowed the simultaneous computation of k off , k* on and k* off . The ... NMR and MS spectrometry at acidic pH in the absence of trypsin, might be the result of a concomitant series of factors. They include the nature of the...

Ngày tải lên: 23/03/2014, 10:21

... 1. Data collection and refinement statistics. Numbers in parentheses correspond to the data in the highest resolution shell. Structure of XAIP Structure of the complex of XAIP and cellobiose Space ... classes of enzyme. The crystal structure of XAIP has been determined at 2.0 A ˚ resolution and refined to R cryst and R free factors of 15.2% and 18.6%, respe...

Ngày tải lên: 29/03/2014, 09:20

... [21], and the more recently published novel two -domain laccases [22–24]. The other phylum of the fungi, the Ascomycota or sac fungi, is much less studied, and only the crystal structure of Melanocarpus ... TaLcc1, and Glu in MaL. In addi- tion, the crystal structure of TaLcc1 does not have a chloride attached to the T2 copper, as observed in the crystal...

Ngày tải lên: 14/02/2014, 18:20