Báo cáo khoa học: Structural studies of thymidine kinases fr

Báo cáo khoa học: Structural studies of thymidine kinases from Bacillus anthracis and Bacillus cereus provide insights into quaternary structure and conformational changes upon substrate binding pot

... and Bacillus cereus provide insights into quaternary structure and conformational changes upon substrate binding Urszula Kosinska 1 , Cecilia Carnrot 2 , Michael P. B. Sandrini 3 , Anders R. Clausen 3 , ... Bacillus thymidine kinase structures FEBS Journal 274 (2007) 727–737 ª 2006 The Authors Journal compilation ª 2006 FEBS 737 Structural studies of thy...

Ngày tải lên : 23/03/2014, 09:21

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Báo cáo khoa học: Structural studies of nucleoside analog and feedback inhibitor binding to Drosophila melanogaster multisubstrate deoxyribonucleoside kinase doc

... speciﬁcity of dNK Earlier crystallographic studies of substrates dT and dC and on the structure of the feedback inhibitor complex with dTTP, as well as mutation studies, have established some of the ... with substrates and a feedback inhibitor [12,13]. It has a structure similar to that of the human dGK and dCK and belongs to a structural family that also contains...

Ngày tải lên : 16/03/2014, 06:20

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Báo cáo khoa học: Structural studies of the capsular polysaccharide and lipopolysaccharide O-antigen of Aeromonas salmonicida strain 80204-1 produced under in vitro and in vivo growth conditions docx

... invasion and bacterial survival. Previous studies have determined the structure of the O-chain polysaccharide of A. salmonicida strain SJ-15 [11]. Partial structure of the core oligosaccharide from ... puriﬁcation of aqueous- and phenol-phase soluble LPS by ultracentrifugation. Crude CPS was recov- ered from the initial 2.5% saline wash of bacterial cells and from...

Ngày tải lên : 23/03/2014, 13:20

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Tài liệu Báo cáo khoa học: Comparative studies of endonuclease I from cold-adapted Vibrio salmonicida and mesophilic Vibrio cholerae docx

... Finnzymes (Espoo, Finland) and Vent and Taq polymerase from Promega were used in the PCRs. Restric- tion enzymes NcoI and SalI were purchased from New England Biolabs (Ipswich, MA, USA), and T4 DNA ligase was ... of VsEndA (Fig. 1). The theoretical pI was 9.61 for VsEndA and 8.62 for VcEndA. Expression and puriﬁcation From 7 L Escherichia coli culture, a total of 24 and...

Ngày tải lên : 19/02/2014, 05:20

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Tài liệu Báo cáo khoa học: Structural features of proinsulin C-peptide oligomeric and amyloid states pptx

... and a wide range of spectroscopic techniques. Suitable methods for investigation of the secondary structure and morphology of such structures, however, require the presence of large amounts of ... distribu- tion of 100 l M proinsulin C-peptide in the presence of divalent Ca 2+ and Mg 2+ ions under native conditions (B), and of 100 l M C-peptide in the presence of i...

Ngày tải lên : 18/02/2014, 04:20

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Tài liệu Báo cáo khoa học: Structural effects of a dimer interface mutation on catalytic activity of triosephosphate isomerase The role of conserved residues and complementary mutations pptx

... dissocia- tion [27] and also in maintaining the geometry of the active site. The availability of crystal structures of TIMs from 21 sources and the large database of TIM sequences from various sources ... by program grants from Department of Biotechnology (DBT), Department of science and technology (DST), Council of Scientiﬁc and Industrial research (CSIR) and se...

Ngày tải lên : 18/02/2014, 11:20

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Tài liệu Báo cáo khoa học: Functional studies of active-site mutants from Drosophila melanogaster deoxyribonucleoside kinase Investigations of the putative catalytic glutamate–arginine pair and of residues responsible for substrate speciﬁcity docx

... mechanism and binding of substrates was investi- gated for the multisubstrate Drosophila melanogaster deoxyribonucleoside kinase. Mutation of E52 to D, Q and H plus mutations of R105 to K and H were ... the ﬁrst structures being solved in 1995 [6,7]. The structure of human thymidine kinase 1 was solved recently, and was shown to belong to a structural family of its...

Ngày tải lên : 19/02/2014, 02:20

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Tài liệu Báo cáo khoa học: Structural characterization of Ca2+/CaM in complex with the phosphorylase kinase PhK5 peptide pdf

... consisting of four copies of four subunits, a, b, c and d. The a and b subunits are regu- latory and are the sites of phosphorylation and meta- bolite binding and are also regulated by the binding of ... substrate binding sites. In the case of the titin and twitchin kinases, the autoinhibitory sequence acts as a pseudo- substrate, occluding ATP binding and...

Ngày tải lên : 19/02/2014, 17:20

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Tài liệu Báo cáo khoa học: "Structural Definition of Affixes from Multisyllable Words" docx

... L. Resnikoff and J. L. Dolby, "The Nature of Affixing in Written English," an algorithm for the structural definition of affixes was developed and applied to data consisting of all ... criterion of four or more words in establishing an affix probability and of two or more consonant strings in defining an affix from a probability was established by Dolby a...

Ngày tải lên : 19/02/2014, 19:20

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Tài liệu Báo cáo khoa học: "Chronometric Studies of Lexical Ambiguity Resolution " doc

... structure of spoken language understanding. Co~nltion, 1980, 8, 1-71. Meyer, D. and Schvaneveldt, R. Meaning, memory, structure, and mental processes. In C.N. Cofer (ed.), The structure of ... verbs, and strongly suggests that theories of lexlcal memory and recognition must begin to take into account the syntacElc functions of worr . 156 Table I Type of Conte...

Ngày tải lên : 21/02/2014, 20:20

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