Báo cáo khoa học: Protein disulfide isomerase family proteins involved in soybean protein biogenesis docx

... al. Soybean protein disulfide isomerase family FEBS Journal 274 (2007) 687–703 ª 2006 The Authors Journal compilation ª 2006 FEBS 703 Protein disulfide isomerase family proteins involved in soybean ... Phylogenetic analyses identify 10 classes of the protein disulfide isomerase family in plants, including single-domain protein disulfide isomer- ase-related...

Ngày tải lên: 23/03/2014, 09:21

... b-con- glycinin in the ER, suggesting that they may play important roles in folding and in formation and rearrangement of disulfide bonds in the storage proteins. Group III PDI family proteins have ... (2007) Protein disulfide isomerase family pro- teins involved in soybean protein biogenesis. FEBS J 274, 687–703. 19 Munro S & Pelham HR (1987) A C-terminal s...

Ngày tải lên: 18/02/2014, 11:20

... Synoviolin increases the mem- brane localization of huntingtin protein [18], also sug- gesting that Synoviolin is involved in intracellular trafficking. We also found that NCT interacts with Synoviolin (Fig. ... also determined the levels of c-secre- tase-unrelated ER protein (calnexin) and cytoskeleton protein (tubulin) in these cells as the internal control proteins. The calnexi...

Ngày tải lên: 23/03/2014, 04:20

... having sufficient power are therefore extremely important in minimizing the in uence of confounding factors arising from inherent problems associated with breeding APP transgenic mice and maintaining ... time-consuming and greatly in uenced by individual handlers. IntelliCages are automated learning cages where animals carrying transponders are housed in groups and trained in learning...

Ngày tải lên: 16/02/2014, 09:20

... with glycosphingolipid-enriched microdomains (PAG), a novel ubiquitously expressed transmembrane adaptor protein, binds the protein tyrosine kinase Csk and is involved in regulation of T cell activation. ... [17–19]. This is in remarkable contrast to cell binding by filipin, another cholesterol-binding reagent. Filipin staining is significantly retained under the same depletion condi-...

Ngày tải lên: 20/02/2014, 03:20

... University) [22,23], containing a cDNA encoding the insulin signal sequence and the Protein A-IgG binding domain, was inserted into NheI-HindIII digested pcDNA3.1, producing the vector, pInsProA. pDN42 ... domain contains two distinct subdomains; a central catalytic domain and a C-terminal lectin-like domain. The central catalytic domain can be further subdivided into two regions. The N-ter...

Ngày tải lên: 08/03/2014, 10:20

... Q 9 and Q 10 , indicating both components are involved in determining the ubiquinone side chain. Thus, we identified the components of the enzymes that determine the side chain of ubiquinone in mammals ... the stationary phase in Luria–Bertani (LB) medium containing ampicillin, and 0.5 mL of the culture was then inoculated into 50 mL of the same medium. A crude protein extract contai...

Ngày tải lên: 16/03/2014, 23:20

... both being in dimer form in low protein concentrations and being reconstituted into tetramers in high protein concentrations. In contrast to the E59Q enzyme, the E59D enzyme has a predominantly ... Fumarate-binding site for human m-NAD-ME. (A) Homotetramer of human m-NAD-ME (Proein Data Bank code: 1PJ3). (B) Fumarate- binding site of m-NAD-ME. The corresponding amino acids in th...

Ngày tải lên: 23/03/2014, 06:20

... quantities of protein utilizing the principle of protein- dye binding. Anal Biochem 72, 248–254. 42 Macheroux P (1999) UV-visible spectroscopy as a tool to study flavoproteins. In Flavoprotein Protocols ... targeting sequence in plant GALDH (FR ⁄ YA). The asterisk (*) marks the histidine residue involved in covalent binding of the FAD cofactor in GUO, ALO and GLO. The FAD-binding...

Ngày tải lên: 23/03/2014, 07:20

... activating domain resulted in preferential inhibi- tion with the analogue containing the aryl moiety of the native reaction intermediate, indicating that DHB- AMS might be more suitable for inhibition ... activating domain DhbE (bacillibactin syn- thesis) and compares it to that of YbtE (yersiniabactin synthesis) upon enzymatic inhibition using a set of newly synthesized aryl sulfamoyl aden...

Ngày tải lên: 23/03/2014, 11:20