... University) [22,23],containing a cDNA encoding the insulin signal sequenceand the Protein A-IgG binding domain, was inserted intoNheI-HindIII digested pcDNA3.1, producing the vector,pInsProA. pDN42 ... domaincontains two distinct subdomains; a central catalyticdomain and a C-terminal lectin-like domain. The centralcatalytic domain can be further subdivided into two regions.The N-terminal ... BamHI and NotI, andwas subcloned into pInsProA, generating the plasmidpInsProADN42 containing truncated rat GalNAc-T1 fusedwith IgG-binding domain of Protein A (P-DN42).Site-directed mutagenesisSite-directed...