Báo cáo khoa học: Mechanism of the ring contraction process in vitamin B12 biosynthesis by the anaerobe Propionibacterium shermanii under aerobic conditions docx

... 2007) doi:10.1111/j.1742-4658.2007.05880.x The mechanism of the ring contraction process during vitamin B 12 biosyn- thesis by the anaerobe Propionibacterium shermanii was investigated under both aerobic and anaerobic conditions by ... cultivated in phosphate Fig. 2. The mechanisms of the ring contraction process in the biosynthesis of...

Ngày tải lên: 23/03/2014, 09:20

... DHNA inhibitors. The binding of the inhibitors to the enzymes cause a decrease in their fluorescence intensities. The increasing fluorescence intensities in Fig. 5A were obtained by subtracting the ... obtained in the absence of the ligand was subtrac- ted from the corresponding data set obtained in the pres- ence of the ligand. The K d values were obtained...

Ngày tải lên: 19/02/2014, 00:20

... represented by the product of the maximum velocity (V max ) and the concentrations of substrates, and the denominator by the function of the concentrations of substrates. The straight lines of the double-reciprocal ... the kinetic parameters, as described in the Appendix, and summarized in Table 1. The calculated K GSH m was the sum of the values...

Ngày tải lên: 08/03/2014, 08:20

... helix–span–helix domain at the C-terminus shown in blue medi- ates dimerization and, together with the basic domain, (red) DNA-binding. ‘*’, amino acids that are identical in all sequences in the align- ment; ... expressed during the preimplantation period, and, with the exception of AP-2a, also in unfertilized oocytes. Furthermore, they determined that functional redun- d...

Ngày tải lên: 16/02/2014, 09:20

... demonstrated by receptor-blocking experiments. The rates of uptake of both the lipid and protein components are not matched by the rate of cel- lular metabolism of these species, resulting in the accu- mulation ... CD36. The accumulation of lipid in these macrophages is accompanied by increased endocytosis and degradation of apoB, with the difference in th...

Ngày tải lên: 19/02/2014, 02:20

... demonstrating the protective role of oscillatory dynamics, the present work concentrates on the mechanism of inactivation of theenzymebyfreeradicalintermediatesandtheroleof aromatic species in this mechanism. The ... help in understanding the origin of the phenomenon. The reason is of course that the changes in concentrations are inherent to the reaction s...

Ngày tải lên: 20/02/2014, 11:20

... glycosylated. Determination of the number of N-glycosylation sites We then investigated the number of N-glycosylation sites and the localization of N-linked chains in ZPA using the purified ZPA. In the 15% gel, the ... mapping on HPLC referring to the reported structures of the unfractionated zona protein mixture [14]. To obtain the core carbohydrate chains of...

Ngày tải lên: 21/02/2014, 03:20

... pro- teins with the exception of LsmDN88p. This suggests that each of the Lsm8p domains contributes to proper U6 binding, either directly or indirectly, by affecting the RNA-binding ability of the ... those with the C-terminal domain deleted is consistent with this (Figs 4 and 5). Hybrid proteins carrying the N-terminus of one protein and the Sm domain of the other...

Ngày tải lên: 07/03/2014, 02:20

... [17,18]. By following the kinet- ics of fibril formation, conditions were defined in which the protein exists in the form of prefibrillar oligomers ⁄ aggregates, which persist during the lag phase. These ... 3.3 bound the most of all, as the signal increase during the injec- tion phase was the largest and there was low dissoci- ation after the end of injection....

Ngày tải lên: 07/03/2014, 17:20

... the kinetic constants for hydrolysis and synthesis of penicillin G, ampicillin and cephalexin. Furthermore, insight into the nature of the transition state in the rate-determining steps of the ... aR145:NH 2 [4,9,34]. In 7-ADCA, which has a dihydrothiazine ring instead of a thiazolidine ring, the 2b-methyl group is not present and due to the more planar character...

Ngày tải lên: 08/03/2014, 08:20