Báo cáo khoa học: Studies of the ATPase activity of the ABC protein SUR1 pptx

... mechanism of cofactor binding as Fig. 6. Progress curves of the reconstitution of EcIPDC with ThDP measured by restoration of the catalytic activity of the formed holo- enzyme for the substrate ... indolepyruvate (Fig. 1). In the course of the establishment of the equilibrium % 85% of the enol form is converted into the ketone (half-time % 8 min at 20 °C) a...

Ngày tải lên: 08/03/2014, 02:20

... bilayers, and their dependence on calcium. Therefore, the objectives of this study were to analyze (a) the effect of KL 4 on the physical properties of membranes, in the absence and presence of Ca 2+ , ... probably involved the extraction of PA from the bilayer at con- centrations of > 100 lm calcium. Thus, the formation of PA–Ca 2+ complexes might explain the...

Ngày tải lên: 30/03/2014, 11:20

... with the higher inhibitory activity of the cEL peptide and the very low inhibitory activity of the cYT peptide compared to other peptides. The flanking residue of the b-turn, i.e. Tyr3 of the cVY peptide ... suggestive of a b-turn in the peptide and the proximity of amide protons due to compact nature of the structure. However, t he coupling constant of...

Ngày tải lên: 19/02/2014, 13:20

... between the two isoforms of the protein, only the molecular mass of 50 kDa would suggest that we had purified the truncated isoform. Cloning of the cDNA of two isoforms of tomato b-fructofuranosidase ... patients. Hence, we concluded that the IgE reactivity of the natural protein mainly depends on the glycan structure. The amino acid sequences of both isofor...

Ngày tải lên: 21/02/2014, 00:20

... assay The LAL test is based on the property of lysates of amebocytes of the horseshoe crab Limulus polyphemus,to form a solid gel in the presence of minute amounts of endotoxins. The comparison of ... answer the question whether the cubic structure observed is of the ÔnormalÕ (right side out) or the inverted type, the geometry of the molecule with its bul...

Ngày tải lên: 21/02/2014, 00:20

... positioning of the aromatic ring of Tyr456 in the closed state. In the absence of a closed complex of E542K P2Ox, the effect of the loss of this hydrogen bond on the packing of the substrate loop is difficult ... those of the native proteins. The second set of spectra were recorded after partial thermal unfolding, whereas the final spectra show the loss...

Ngày tải lên: 07/03/2014, 03:20

... as a result of the decrease in pH was assayed by measuring the radioactivity of tritium, as described in Materials and methods (In the figure, the y-axis represents the cpm values of pellet). ... Ca 2+ channel in the ER, which increases Ca 2+ leakage via a mechanism dependent on both the pH and the C-terminal cytosolic region of the protein [9]. However, the preci...

Ngày tải lên: 29/03/2014, 23:20

... extent of ATPase activity at NBD1 and NBD2 remains the same in full-length SUR1 (i.e. that of NBD1 is  20% of that of NBD2), then the marked inhibition of ATPase activity of SUR1 suggests that the ... filtration, especially in the case of SUR1F, diluted the amount of protein available and was therefore not used routinely. However, no difference in the...

Ngày tải lên: 23/03/2014, 09:20

... interruption of the geometry of the recognition helix for either Antp or the TTF-1 HD, the anomalous amide exchange pattern and the NOE connectivity data of the C-terminal portion of helix III ... inertia. In the context of RSDM, the detailed analysis of the three spectral densities J(0), J(x N ) and J(0.87x H ) allowed us to obtain a rather complete description...

Ngày tải lên: 18/02/2014, 16:20

... one to develop a broader perspective of the questions within an analysis of the hypothesis in the con- text of a characterization of the "how" of the entire behavior. By adapting ... a result of acti- vation of a pragmatic representation of a disambiguated word meaning. It is the in interaction of the results of these asynchronous processes...

Ngày tải lên: 21/02/2014, 20:20