Báo cáo khoa học: Characterization and structural modeling of a new type of thermostable esterase from Thermotoga maritima ppt

... 2007 The Authors Journal compilation ª 2007 FEBS Characterization and structural modeling of a new type of thermostable esterase from Thermotoga maritima Mark Levisson, John van der Oost and Serve ´ W. ... hydrolases and share structural and functional characteristics, including a catalytic triad, an a ⁄ b- hydrolase fold and a cofactor independent...

Ngày tải lên: 23/03/2014, 09:20

... LTFS and ALE, these four components, a partial order of types, a setoffeatures,appropriatenessdeclara- tions and type- antecedent constraints can be taken as the signature of a grammar, relative ... which descriptions can be interpreted. LTFS and ALE also make several assump- tions about the structure and interpretation of this partial order of types and about appropr...

Ngày tải lên: 23/03/2014, 19:20

... Dianzani I, Lattanzi P, Spada M, Romano V, Calı ` F, Andria G, Ponzone A, Marra E & Piazza A (2001) Genetic heterogeneity in five Italian regions: analysis of PAH mutations and minihaplo- types. ... Italy. The study was partly supported by Agenzia Italiana del Farmaco (AIFA grant FARM5MATC7), Rome, Italy. We thank Jean Ann Gilder for revising and editing the text and Anna Nastas...

Ngày tải lên: 16/03/2014, 01:20

... phosphorylase from the Archaeon Pyrococcus furiosus Giovanna Cacciapuoti 1 , Sabrina Gorassini 1 , Maria Fiorella Mazzeo 2 , Rosa Anna Siciliano 2 , Virginia Carbone 2 , Vincenzo Zappia 1 and Marina ... (time-zero control) and at different time inter- vals, aliquots were removed from each sample and analyzed for activity in the standard assay. Activity values are expressed as a pe...

Ngày tải lên: 23/03/2014, 09:21

... spectra and peak fitting was performed using grams ai software (ThermoGalactic, Salem, NH). Lorentzian curves were fitted to the amide I band of the PrP signal and assigned to a secondary structure type according ... with a mercury cadmium telluride detector. Data are at a resolu- tion of 4 cm )1 and are an average of 1024 spectra collected at room temperature (21 °C). The w...

Ngày tải lên: 23/03/2014, 10:21

... homodimers and dissociates in membrane and soluble subcomplexes; isolation of the Ndh complex by BN / PAGE and Tricine / PAGE Intact MS and BS chloroplasts were used as starting material for the separation ... chloroplasts were separated in CN ⁄ PAGE (1D, the hori- zontal bands) and then in nondenaturing conditions by BN ⁄ PAGE (2D). The molecular mass standards and direction o...

Ngày tải lên: 23/03/2014, 13:20

... Functional and structural analyses of N-acylsulfonamide- linked dinucleoside inhibitors of RNase A Nethaji Thiyagarajan 1 , Bryan D. Smith 2, *, Ronald T. Raines 2,3 and K. Ravi Acharya 1 1 Department ... development and use of N-acylsulfonamides and sulfonimides as antagonists of nucleic acid-binding proteins. Database Structural data for the two RNase A complexes are...

Ngày tải lên: 14/02/2014, 22:20

... exopolygalacturonase from Thermotoga maritima FEBS Journal 272 (2005) 5464–5473 ª 2005 FEBS 5473 Characterization and mode of action of an exopolygalacturonase from the hyperthermophilic bacterium Thermotoga ... members of family 28 of the glycoside hydrolases, including the endopolygalacturonases, exo- polygalacturonases and rhamnogalacturonases [3,4]. Although a ha...

Ngày tải lên: 20/02/2014, 03:20

... (5¢- GATGTCACGCAGAGTGAGCAGGTAG-3¢)/TRHR-7 (5¢-GAGACCATACAGAAC-C-3¢); second set, A5 2 (5¢- AGAGTGAGCAGGTAGCGAGAGGAG-3¢)/TRHR-8 (5¢-GGGGGTGTAGAGGTTTCTGGAGAC-3¢); third set, A5 3 (5¢-CGAGAGGAGCATTAGA-TAGATG CAG-3¢)/TRHR-9 (5¢-GCCGAAATGTTGATGCCCA GATAC-3¢) (Fig. 1C). ... (5¢-ATAATGGATAA CGTAACTTTTGCTG-3¢)/TRHR1-4 antisense (5¢-TC TGTTAAATGTACCTAAGTAGGCA-3¢)andTRHR2-2 sense (5¢-CAGCAAAATGGAAAAT...

Ngày tải lên: 21/02/2014, 03:20

... post-treatment. The poly (A) -RNA was extracted from the larvae, and 300 ng mRNA of each sample was loaded on a gel. Actin mRNA served as an internal marker to equate mRNA quantities. Fig. 9. Alignment ... reduced, and JH is inactivated via catabolism by JHEH and JH-specific esterase. JHEH was purified from Manduca sexta [24], and the JHEH gene was isolated from M. sexta [25]...

Ngày tải lên: 07/03/2014, 21:20