Báo cáo khoa học: Identification of substrates for transglutaminase in Physarum polycephalum, an acellular slime mold, upon cellular mechanical damage ppt

... chemical damage of Physarum microplasmodia by treatment with ethanol or detergent results in transamidation of actin and CBP40 [20]. Mechanical damage also resulted in the crosslinking of CBP40 to form ... sequence analysis of horseshoe crab cuticular proteins and their involvement in trans- glutaminase-dependent cross-linking. FEBS J 272, 4774–4786. F. Wada et al. Tran...

Ngày tải lên: 23/03/2014, 09:20

... (B) and Western blotting (C). Lane 1, cellular protein of E. coli transformed with a control vector; lane 2, cellular protein of E. coli transformed with the vector harbouring PpTGase cDNA; lane ... stringency. Expression of recombinant PpTGase in E. coli Recombinant proteins were produced using partial C-terminal region (PpTGase-C: corresponding to 554Gly)855Val) and full-length...

Ngày tải lên: 17/03/2014, 23:20

... membrane proteins of the INM. The INM proteins form complexes, transiently or stably, with lamins, chromatin proteins and a vari- ety of regulatory proteins, including transcriptional regulators and ... mislocalization of emerin that is caused by the loss of emerin binding to lamin A (X- linked form) or by the loss of lamin A (autosomal dominant form) [22,23]. Several emerin binding...

Ngày tải lên: 07/03/2014, 12:20

... DNA-binding motif in nucleolin [21– 27]. The RGG domain of FMRP, which is an RNA- binding protein involved in nerve cell differentiation, interacts with the G-quartet forming RNA [22–25]. In addition, ... RBD and the RGG domain of nucleolin, a DNA-binding protein contributing to the transcrip- tion of ribosomal RNA, bind to the G-quadruplex forming ribosomal DNA [26]. Moreover,...

Ngày tải lên: 15/02/2014, 01:20

... pWW115, Shengqing Yu for advice on the pulmonary clearance assay, R. Morell for help with DNA sequencing, Yandan Yang for help with Southern blotting, and Lina Zhu and Yili Chen for help in manuscript ... Zeocin resistance cassette Invitrogen pSlpxX-zeo Zeocin resistance gene inserted into pSlpxX This study pUC4K Kanamycin resistance cassette Amersham pSlpxL-kan Kanamycin resistance...

Ngày tải lên: 18/02/2014, 14:20

... known to involve major adjustments in the expression of ribosomal genes and genes coding for proteins involved in RNA metabolism and protein synthesis. In fact, protein synthesis in marine snails was ... slightly less and later in mantle. One inducible and two constitutive isoforms of HSP70 are synthesized in the gills and mantle of C. gigas [26]. The expression level of...

Ngày tải lên: 18/02/2014, 16:20

... determine the calcium-binding domain of the protein. (A) Schematic drawing of the recombinant proteins. Six GST-fused recombinant proteins containing the three distinctive domains of tandem repeat 1 ... band was obtained after digestion Fig. 3. 45 Ca overlay analysis of fusions of GST and recombinant OMM-64 variants (rOMM-64-I-V and -C), containing different domains of the protein...

Ngày tải lên: 18/02/2014, 17:20

... C30 terpe- nes and include a number of active constituents of medicinal plants, as exemplified by glycyrrhizin in Glycyrrhiza glabra, ginsenosides in Panax ginseng, sai- kosaponins in Bupleurum ... extracts of these trans- formants with 14 C-labeled b-amyrin as a substrate did not show any hydroxylase activity, and in vivo assay by feeding the same substrate to the culture of e...

Ngày tải lên: 19/02/2014, 07:20

... Characterization of the recombinant extracellular domains of human interleukin-20 receptors and their complexes with inter- leukin-19 and interleukin-20. Biochemistry 42, 12617– 12624. 43 Sheikh F, Baurin ... by Canadian Institutes of Health Research Grant MOP 15094 (to E.N.F.); National Science and Engineering Research Council of Canada (NSERC) Grant 203833-02, the Institute for Ro...

Ngày tải lên: 19/02/2014, 07:20

... FEBS 627 Identification of membrane-bound serine proteinase matriptase as processing enzyme of insulin-like growth factor binding protein-related protein-1 (IGFBP-rP1/angiomodulin/mac25) Sanjida ... follows: aprotinin, leu- peptin, pepstatin A, AEBSF and proteinase inhibitor mixture that contains AEBSF, aprotinin, bestatin, E-64, leupeptin and pepstatin A from Wako Pure Chemical Industri...

Ngày tải lên: 19/02/2014, 07:20