Báo cáo khoa học: The unique pharmacology of the scorpion a-like toxin Lqh3 is associated with its flexible C-tail pdf

... pharmacological features of scorpion a-like toxins are associated with the flexibility of the C-tail. Results The bioactive surface of Lqh3 Twenty-four residues were substituted and the toxin mutants were ... al. a-Like toxin binding is linked to its flexibility FEBS Journal 274 (2007) 1918–1931 ª 2007 The Authors Journal compilation ª 2007 FEBS 1931 The...

Ngày tải lên: 23/03/2014, 09:20

... the alternative splice site in Meis3, such that both the Meis3.1 and Meis3.2 isoforms were amplified. The relative amount of each splice form as a percentage of the total Meis3 is plotted in the ... for Meis2d. However, the activity of the full-length Meis1a was not tested, and on the basis of our work we expect that its activity would be inhibited by the conserve...

Ngày tải lên: 16/02/2014, 15:20

... of a severe mutant. At the other end of the spectrum, where we have low energy, there is 75% probability for the mutation to be nonsevere if the energy is 0.125 or lower. Thus, on the basis of ... Analysis of these screens has led to the finding that the prevalence of missense somatic mutations is far more frequent than expected. Moreover, this observation has bee...

Ngày tải lên: 18/02/2014, 11:20

... by Southern analysis. The rluD::cat strain had a full deletion of both rluD and yfiH, together with their annotated pro- moter sequences. The inserted cat cassette had a distance of 90 nt from the ... directed isomerisarion of uridines in the helix-loop 69 of 23S rRNA occurs as a late event during assembly of the ribosomal large subunit, but before the 50S subunit enter...

Ngày tải lên: 18/02/2014, 16:20

... l M) against the inverse agonist activity of 4-OHT (1 and 10 lM). The assay set marked with an asterisk shows the the inverse antagonist activity of BPA for 1 l M 4-OHT, and the other set marked ... the b-carbon of the Ala side chain, whereas Arg has -CH 2 CH 2 NHCH(¼NH)NH 2 . These groups are capa- ble of making hydrogen bonds with the phenol-hydro- xyl group of B...

Ngày tải lên: 18/02/2014, 16:20

... castellii sequence is small compared to that achieved by location of the full-length extension at the N-terminal end of the polypeptide. The benefit of these alterations at each extremity of the polypeptide chain, ... the polypeptide chain, must of necessity arise from contacts that are made outwith the active site of the enzyme. The effect of removal of th...

Ngày tải lên: 19/02/2014, 00:20

... to the A-state may be altered. This hypothesis is supported by data obtained from comparison of the kinetic behavior of the mutants with that of the acid-denatured protein in the reaction with ... however, that the signal is stronger than that recorded in the presence of monovalent ani- ons [17,18]). The intensity of the 416 nm dichroic band is $ 35% that...

Ngày tải lên: 19/02/2014, 05:20

... samples 11–14 correspond to QAE isoforms of nfeAFP, whereas the others correspond to SP iso- forms, and the TH activity of the QAE isoform is higher than that of the SP isoform. Fig. 6. TH activity ... is the species that cannot exert substantial TH activity by itself; (c) although the antifreeze activity of the SP isoform is low, it can exert TH activity with t...

Ngày tải lên: 19/02/2014, 16:20

... plants [31,44–47]. This mechanism involves the N-terminal part of CGS. The time constant of this control is unknown. If this time constant is much larger than the time constant of the kinetic controls ... branch-points display a very large diversity in terms of the number of the enzymes involved, the kinetic mechanisms of the competing enzymes and the number...

Ngày tải lên: 20/02/2014, 02:21

... tuples were discarded. The precision of the tuples discovered by the different seed-sets in the last iteration of our algorithm are in Table 3. These results reveal that the precision of har- vested ... that the precision of tuples extracted from both the English and Dutch corpora by the gen- eral set of mixed seeds was as high as the max- imum precision obtained b...

Ngày tải lên: 20/02/2014, 04:20