Báo cáo khoa học: Structural requirements for antimicrobial versus chemoattractant activities for dermaseptin S9 pptx

... al. Antimicrobial and chemotactic dermaseptin S9 FEBS Journal 275 (2008) 4134–4151 ª 2008 The Authors Journal compilation ª 2008 FEBS 4151 Structural requirements for antimicrobial versus chemoattractant ... Drs S9 has formed self-associ- ated oligomers with potent antimicrobial activity. D7, after 7 days, Drs S9 has formed amyloid-like fibrils. C. Auvynet et al. Antim...

Ngày tải lên: 23/03/2014, 07:20

... Journal 277 (2010) 3003–3013 ª 2010 The Authors Journal compilation ª 2010 FEBS 3013 Structural requirements for Caenorhabditis elegans DcpS substrates based on fluorescence and HPLC enzyme kinetic ... HIT motif is critical for catalysis [14,20]. This histidine is involved in the formation of a covalent nucleotidyl phosphohist- idyl intermediate, the nucleophilic agent for the c-phos...

Ngày tải lên: 29/03/2014, 09:20

... complex. Science 284, 812–815. 1876 A. T. Nies et al. (Eur. J. Biochem. 269) Ó FEBS 2002 Structural requirements for the apical sorting of human multidrug resistance protein 2 (ABCC2) Anne T. Nies 1 ,Jo¨rg ... °Cfor1minandrehydratedin NaCl/P i . C ells were incubated w ith the primary antibody for 60 min at room temperature, washed three times with NaCl/P i , incubated with the second...

Ngày tải lên: 31/03/2014, 09:20

... good model for performing structural studies. In the present study, we therefore used SDS to structurally character- ize the oligomerization process of C-peptide and ana- lyzed the formation of ... whereas formamide breaks hydrophobic ones. The addition of 50–150 mm NaCl reduced the oligomer formation of C-peptide (Fig. 1D) and form- amide had no effect (Fig. 1D). The combined results the...

Ngày tải lên: 18/02/2014, 04:20

... reductase (PET- NR) from Enterobacter cloacae. For reference, in Table 1, we summarize the flavoproteins for which a specific H-tunnelling study has been performed. Gener- ally, the KIEs of H transfer ... NAD(P)H binding and dissocia- tion can be measured by following the formation of this CT absorbance at, for example, 555 nm while perform- ing a concentration dependence [35,39]: k obs ¼...

Ngày tải lên: 18/02/2014, 11:20

... long-term function-oriented structural effort aimed at understanding the molecular bases of polyQ disease proteins, we report here a study of the structural determinants for the interactions of Anp32a with ... London) for providing the Lanp clone, which was produced from a cDNA originally provided by Dr A. Matilla (ICH, London), Filippo Prischi for help with the gromacs software, an...

Ngày tải lên: 18/02/2014, 17:20

... cleft formed by the two domains. A common functional conformational change has been reported in this protein family, which involves switching from an open form to a sugar-transporter bindable form, ... designated a closed form. The TvuCMBP–c-cyclodextrin complex structurally resembles the closed form of EcoMBP, indicating that TvuCMBP complexed with c-cyclodextrin adopts the closed form. The...

Ngày tải lên: 19/02/2014, 00:20

... deconvolu- tion program for each p26 variant generated by site-directed mutagenesis and for purified wild-type (WT) p26 from transformed Escherichia coli and Artemia embryos. Structural elements R110G (%) F112R (%) R114A (%) Y116D (%) WT (E. ... Sequencing Facility, Center for Applied Genomics, Hospital for Sick Children, Toronto, ON, Canada). Bacterial synthesis and purification of p...

Ngày tải lên: 07/03/2014, 12:20

... transcriptase (Promega) at 37 °C for 1 h in the appropriate buffer. The reaction was stopped by incubation at 70 °C for 10 min. iQ TM SYBR Green supermix PCR kit (Bio-Rad) was used for real time monitoring ... well), incubated at 28 °C for up to 24 h and then exam- ined by microscopy for phenotype analysis. Unfertilized eggs were removed after 4 h. Alternatively, embryos were fixed...

Ngày tải lên: 07/03/2014, 21:20

... Biochem 150, 76–85. T. Meier et al. Structural evidence for a constant c 11 ring stoichiometry FEBS Journal 272 (2005) 5474–5483 ª 2005 FEBS 5483 Structural evidence for a constant c 11 ring stoichiometry in ... feature for function [14]. The number of binding sites on the c ring determines the ATP to proton ⁄ Na + ratio, and therefore this stoichiometry is an important bioenergetic...

Ngày tải lên: 07/03/2014, 21:20