Báo cáo khoa học: The gp130⁄STAT3 signaling pathway mediates b-adrenergic receptor-induced atrial natriuretic factor expression in cardiomyocytes ppt

... protein kinase (MAPK) signaling pathway [15,16]. On the other hand, the MAPK pathway has been shown to have an important role in the regulation of STAT3 signaling [17,18]. We thus examined the ... in the NRCM culture medium (Fig. 4C). The involvement of STAT3 in b-AR-induced ANF expression was further supported by investigation of the instant upstream signaling...

Ngày tải lên: 23/03/2014, 07:20

... genes were found to be gathered in a large operon called the cip-cel cluster, beginning with the gene encoding the scaffolding pro- tein named cellulosome-integrating protein C (CipC), followed ... attributed to the physical prox- imity of the enzymes in the mini-cellulosomes and to cellulose targeting of the complexes via the CBM of the mini-scaffoldin [21]. The most...

Ngày tải lên: 18/02/2014, 08:20

... nucleotide-binding sites (site 1 and site 2) that comprise the Walker A and Walker B motifs of one NBD and the linker domain of the other. In the absence of Mg 2+ , there is little difference in ATP ... (above the average of the individual NBDs), indi- cating that the NBDs must interact, and emphasizing the functional importance of the last 42 amino acids of SUR2. One po...

Ngày tải lên: 06/03/2014, 11:20

... are involved in the correct folding of proteins and ⁄ or the removal of incompletely synthes- ized, damaged or malfolded proteins in the different compartments of the cell [1–4]. By studying the ... fold efficiently into their native conformation at the membrane–cell wall interface [6]. Thereafter, they can pass the cell wall in order to be released into the growth medium...

Ngày tải lên: 07/03/2014, 12:20

... of the reaction network co mprising redundant routes. This discrepancy points to another problem inherent in the theoretical concept: if there are redundant reactions or pathways, the flux-minimization ... f the reaction, the larger the weighting factor for the backward reaction. A linear programming technique is applied to m inimize the total flux at fixed values of the ta...

Ngày tải lên: 07/03/2014, 15:20

... pmolÆmg )1 protein of Rf being present in the mitochondrial sus- pension. When the FMN concentration was increased to 50 lm (Fig. 5D), the amount of FAD increased almost linearly in the first 10 min of the ... pmolÆmin )1 Æmg )1 protein. The amount of FAD reached a maximum value of 3600 pmolÆmg )1 protein within 15 min of incubation. Prolonging the incubation time resulted i...

Ngày tải lên: 16/03/2014, 04:20

... regulating the cGMP affinity of the PDE11A4 tandem GAF domain and thus allosterically affect PDE11 activity. The role of the N-terminus of the PDE11A4 GAF tandem domain The N-termini that precede the ... that the N-terminus profoundly affects GAF signalling [27]. We therefore probed whether the 196- residue N-terminus which precedes the hPDE11A4 GAF tandem affects intramole...

Ngày tải lên: 16/03/2014, 06:20

... arginines in each subunit. The pH profile data suggest that as the pH increases, there is still sufficient protonation of the lysines at pH 10, along with the fully protonated arginines, to interact with ... differences in staining intensity in lectin binding studies. The lectins SNA/ MAA appeared to have a higher affinity for skin tryptase than for lung tryptase. In contrast,...

Ngày tải lên: 31/03/2014, 07:20

... adi- pogenesis and increased insulin sensitivity. The present study provides the first evidence that PGD 2 participates in the differentiation of adipocytes and in insulin sensitivity in vivo, and the H-PGDS ... washing, the sections were incubated with the biotinylated goat anti- (rabbit IgG) for 30 min (Vector Laboratories, Burlingame, CA, USA), and this was followed by sta...

Ngày tải lên: 16/02/2014, 09:20

... any old finding predicted by the attending physician; a 'new unex- pected finding' was any new finding not predicted by the attending physician; and an 'old unexpected finding' ... reviewed by the team at 10:00 hours, when the radiologist communicated any positive findings. The following definitions were used: a 'new expected finding' was any new findin...

Ngày tải lên: 25/10/2012, 10:39