Báo cáo khoa học: Identiﬁcation of a glycosphingolipid transfer protein GLTP1 in Arabidopsis thaliana ppt

... The cDNA was ampliﬁed fromU66003 using primers U660035Eco2 (5¢-AATAGAGAATTCAGAGAAAGAGATACGAGATGGA-3¢) andU660033Not (5¢-TCATAAGGCGGCCGCCTACATCGATCTTAATCTGCTCAA-3¢). A fragment carryingAt3g21260 ... (5¢-AGACTGCTCTAGAATGGGTTTCTAAACCAACACGT-3¢) and GLTP1PRON-BAM (5¢-CTCCTTGGATCCGCCTGAGAATTGAAAAAGGTGGG-3¢). A 1.3 kb fragment carrying the At1g21360promoter was ampliﬁed using primers 21360PRUXBA(5¢-AACGATCTAGATTAAGAATGTAATCACATTAGGGT-3¢) ... modelingenvironment.Histochemical GUS activity assays A 1.8 kb DNA fragment carrying the At2g33470 promoterwas ampliﬁed from the A. thaliana Col-0 genome usingprimers GLTP1PROUXBA (5¢-AGACTGCTCTAGAATGGGTTTCTAAACCAACACGT-3¢)...

Tài liệu Báo cáo khoa học: Identiﬁcation of a novel matrix protein contained in a protein aggregate associated with collagen in ﬁsh otoliths pdf

... 3.45Ca overlay analysis of fusions of GST and recombinantOMM-64 variants (rOMM-64-I-V and -C), containing differentdomains of the protein, to determine the calcium-binding domain of the protein. ... was found to have a molecular mass of 64 kDa, and to containtwo tandem repeats and a Glu-rich region. The structure of the protein and that of its DNA are similar to those of starmaker, a protein ... andendo -a- N-acethylagalactosaminidase (70 munits, E). The HMWaggregate was digested only by heparitinase II (arrowhead), and a 64 kDa protein band appeared instead (arrow). Some non-speciﬁcbinding was observed...

Tài liệu Báo cáo khoa học: Identiﬁcation of Ewing’s sarcoma protein as a G-quadruplex DNA- and RNA-binding protein ppt

... Ewing’s sarcoma (EWS) oncogene contains an N-terminal transcrip-tion activation domain and a C-terminal RNA-binding domain. Althoughthe EWS activation domain is a potent transactivation domain ... pGEX–EWS; EAD forward d(CGGAAT TCA TGG CGT CCA CGG ATT ACA G) and EADreverse d(CGC TCG AGT CAT CCG GAA AAT CCTCCA GAC T), for pGEX–EAD; RGG1 forward d(CGGAAT TCC CAG GAG AGA ACC GGA GCA T) andRGG1 ... protein as a G-quadruplex DNA- and RNA-binding protein Kentaro Takahama1,*, Katsuhito Kino2,*, Shigeki Arai3, Riki Kurokawa3and Takanori Oyoshi11 Department of Chemistry, Faculty of Science,...

Báo cáo khoa học: Identiﬁcation of a novel inner-core oligosaccharide structure in Neisseria meningitidis lipopolysaccharide docx

... [14]whereas the NM115 strain was isolated from patients withmeningococcal sepsis [15]. Strain 1000 was initially identiﬁed in a collection of meningococcal clinical isolates by virtue of its lack of ... (Lipid A- OH) is as indicated. Lipid A- OH consists of two glucosamine residues each bearing an N-linked 3-OH C14:0fatty acid and a phosphate group. Variation in lipid A- OH sizes observed are dueto ... clinicalisolates that were not reactive with these mAbs. Structuralanalysis of meningococcal clinical isolates revealed anadditional and uniquely located glucose residue in the coreoligosaccharide...

Báo cáo khóa học: Identiﬁcation of a gene encoding Lon protease from Brevibacillus thermoruber WR-249 and biochemical characterization of its thermostable recombinant enzyme pptx

... geneproduct, a DNA-binding protein. Proc.NatlAcad.Sci.USA78,2043–2047.13. Neuwald ,A. F.,Aravind,L.,Spouge,J.L.&Koonin,E.V.(1999)AAA+: a class of chaperone-like ATPases associated withthe assembly, ... The standard curve was plotted with the logarithm of molecular mass against Ve/V0 of the standard protein. Peptidase and ATPase assaysThe peptidase activity of Bt-Lon was examined as describedpreviously ... ellipticity at 222 nm. The fractions of native protein obtained after a two-state analysis of the data (seeMaterials and methods) are shown as a function of temperature. In (A) and (B), the units of...

Báo cáo khoa học: Identiﬁcation of a novel binding site for calmodulin in ammodytoxin A, a neurotoxic group IIA phospholipase A2 docx

... GGAATTCGCgGCcGCCtTGTCACACTCACAAATCCGATTCTC3+wtGGGCAAGCTTGCgGCcGCAATCTGCTTTCGAcAGAATCTGAAcACATAttcgAAAAAGTATATGC3+YIRNGGGCAAGCTTGCgGCcGCAATCTGCTTCCGAcAGAATCTGAAcACATACAgCTATATATATAGG3– GGAATTCTGCAGTTAGCATTTgagCTCtccCTTGCACAAgAAGTCCGGÓ FEBS 2003 A novel ... GGGCAAgcttgCTaTTcCCTCCTACTCCTcTTACGGATGCTACTGCGGCtgGGGGGG 2a+ GACTGCAaCCCCAAAtCGGACAG2b+ CAAATACaAgCGGGtGAACGGGGCTATCGTCTGTGaAAAAGGC2– GGAATTCGCgGCcGCCtTGTCACACTCACAAATCCGATTCTC3+wtGGGCAAGCTTGCgGCcGCAATCTGCTTTCGAcAGAATCTGAAcACATAttcgAAAAAGTATATGC3+YIRNGGGCAAGCTTGCgGCcGCAATCTGCTTCCGAcAGAATCTGAAcACATACAgCTATATATATAGG3– ... proteins,such as the gating domain of a Ca2+-activated K+channel[40] and the C-terminal part of toxic Bacillus anthracisoedema factor [41], suggest that interaction of CaM withlarger proteins...

Báo cáo khoa học: Identiﬁcation of a putative triacylglycerol lipase from papaya latex by functional proteomics pdf

... 5¢-ACTCAAATCACTAGTATTCTTCCACCA-3¢and 5¢-CATTTGAACATAAACATGAACAAATAAGTT-3¢and the following conditions: annealing temperature 55 °C,25 cycles, Phusion polymerase used according to themanufacturer’s ... home-made databases thatcontain either a six frame-translation of C. papaya ESTs or a six-frame translation of C. papaya whole genome shotgunsequences. These databases were constructed using transeq,which ... informationThe following supplementary material is available:Fig. S1. Identiﬁcation of proteins contained in spot 5by de novo sequencing and screening a GenBank non-redundant protein database...

Báo cáo khoa học: Identiﬁcation of a copper-repressible C-type heme protein of Methylococcus capsulatus (Bath) A member of a novel group of the bacterial di-heme cytochrome c peroxidase family of proteins docx

... N-Terminal processing would lead to a mature protein of 732 amino acids with a theoreticalmolecular mass of 78 kDa. A search in the PROSITEdatabase of protein families and domains [12] withMCA2590 ... GGCAACGAGCAAGGTCCGAAGsapE–R 2590 R GACGTCGTGAGTGCCTCCGTGmopB–F 3103 F CGACGTGCAGTATTACTTTTCTAGGGmopB–R 3103 R AGTATCAAACCGTGCTGGTCTCCHeme protein identiﬁcation in M. capsulatus O. A. Karlsen ... buried amino acids, therebymaintaining the structural integrity of an N. europaeaCCP-similar fold of SACCP, and thus bringing thehemes in close proximity to each other in contrast toincreased interheme...

Báo cáo khoa học: Identiﬁcation of a novel alternative splicing variant of RGS5 mRNA in human ocular tissues ppt

... for angiotensin II receptor (AT 1a) cDNAcloning: 5¢-CGCGGATGAAGAAAATGAAT-3¢ (forward);5¢-CCCTTTGGAAACTGGACAGA-3¢ (reverse).Primers used for cannabinoid receptor-1 (CB-1) cDNAcloning: 5¢-GAGGACCAGGGGATGCGAAGG-3¢;5¢-TGCCCCCTGTGGGTCACTTTCT-3¢.Plasmids ... expression pattern,cellular localization and functions of RGS5s suggest that RGS5s may play a critical role in the regulation of intracellular signaling pathways.AbbreviationsAT1, angiotensin II ... Identiﬁcation of a novel alternative splicing variant of RGS5 mRNA in human ocular tissuesYanbin Liang, Chen Li, Victor M. Guzman, William W. Chang, Albert J. Evinger III, Dyna Saoand David...

Báo cáo khoa học: Identiﬁcation of a 250 kDa putative microtubuleassociated protein as bovine ferritin Evidence for a ferritin–microtubule interaction pdf

... 4,bovine liver ferritin; lanes 2 and 5, bovine adrenal gland ferritin; andlanes 3 and 6, the 250 kDa protein. Identiﬁcation of a putative MAP as ferritin M. R. Hasan et al.824 FEBS Journal 272 ... electrophoreticmobility of bovine liver ferritin, bovine adrenal cortexFig. 1. Amino acid sequence analysis of the 250 kDa protein. A cyanogen bromide digest of the 250 kDa protein was separated bySDS ⁄ PAGE, and ... Department of Bioscience and Bioinformatics, Kyushu Institute of Technology, Iizuka, Fukuoka, Japan2 Department of Biological Sciences, Faculty of Science, Kanagawa University, Hiratsuka, Kanagawa, JapanAlthough...

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