Báo cáo khoa học: Alternative binding proteins: Anticalins – harnessing the structural plasticity of the lipocalin ligand pocket to engineer novel binding activities pdf

... MINIREVIEW Alternative binding proteins: Anticalins – harnessing the structural plasticity of the lipocalin ligand pocket to engineer novel binding activities Arne Skerra Lehrstuhl ... framework of the paired variable domains of the light and heavy chains. These CDRs come together at the tips of the Y-shaped mole- cule to form a contiguo...

Ngày tải lên: 23/03/2014, 07:20

... Loss of the LBD abolished the ability of C-Vrp1p 364)817 to restore growth of vrp1D cells at elevated temperature. We next assessed the importance of the LBD for the ability of C-Vrp1p 364)817 to ... in yeast [55]. Binding to WIP serves to protect WASP from degradation [5 5–5 7]. In another study, WIP binding inhibited the ability of the WASP-family pro...

Ngày tải lên: 18/02/2014, 16:20

... structure [ 2–9 ,1 1–1 5,3 8–4 1,9 5–9 9,111], although the length of this domain is variable within the family. The final partial tree, the ÔSBD treeÕ, lacks the character of a tree consisting of two groups, ... With regard to the Nostoc CGTase, it matches best the a-amylase from Streptomyces griseus, a representative of the a-amy- lases produced by streptomy...

Ngày tải lên: 08/03/2014, 08:20

... control) 0 25 50 75 100 125 148 98 64 + – KO WT Tg – + 22C11 148 98 64 Aβ rodent + – KO WT Tg – + E 148 98 64 C8 + – KO WT Tg – + F 36 anti-GAPDH + – KO WT Tg – + APPsα total (% of control) Fig. 1. Levels of total APPs are ... translate into increased amounts of APP or APLP2 CTFs. Therefore, it is reasonable to suppose that the levels of CTFs are re...

Ngày tải lên: 15/03/2014, 10:20

... of the period and amplitude of Src oscillations by the activities of the activatory phosphatase RPTP and inhibitory kinase Csk. Dependence of the oscillation amplitude (A) and period (B) on the ... presenting the pulse of Src activity in the plane of the inactive and active fractions, s i and s 1 . If the duration of the stimulus exceeds the critical valu...

Ngày tải lên: 18/02/2014, 11:20

... the peptide is bound in a concentrated manner at the top of the column, or, if bound to the resin prior to packing the column, the salt gradient concen- trates the peptide on its way out of the ... Ab( 1–4 0) and Ab( 1–4 2) coding sequences together with added ATG codons were cloned directly into a Pet vector to facilitate production of Met-Ab( 1–4 0) and Met...

Ngày tải lên: 18/02/2014, 13:20

... has Fig. 5. Analysis of the enzymatic activity of His-CoaB R206Q and A276V. The synthesis of 4¢-phosphopantothenoyl-CMP (–cysteine, upper part of the figure) and of 4¢-phosphopantothenoylcysteine (+ ... identified and shown to be involved in the second half reaction of the (R)-4¢-phospho-N-pantothenoylcysteine synthetase. The proposed intermediate of the (R)-4¢-pho...

Ngày tải lên: 19/02/2014, 12:20

... using the SV total RNA isolation system (Promega, Madison, WI, USA), according to the manufacturer’s protocol. Paramagnetic beads were used to isolate mRNA from the pool of total RNA. The purified mRNA ... another major GEF with activity towards Ras. Ras-GRF1 mediates activation of Ras subsequent to the stimulation of G i - and G q - coupled receptors [8,9]. The main me...

Ngày tải lên: 19/02/2014, 18:20

... the scale of the Bard’s genera- tivity, and the quality of its insights. 5 Empirical Evaluation The vastness of the web, captured in the large- scale sample that is the Google ngrams, means the Jigsaw ... can one make playful – and creative – allusions to the writings of others, or 14 mischievously subvert the conventional wisdom of platitudes and clich...

Ngày tải lên: 20/02/2014, 05:20

... hows that the b FGF r egulation of their expression speci®cally depends on the nature of HSPG and of the Sertoli cell developmental stage. In conclusion, HSPG are partners and the target of bFGF in ... for the recognition of the speci®c bFGF receptor, leading to the formation of a ternary complex comprising HSPG±bFGF±FGFR. These oligosaccharidic motifs are differen...

Ngày tải lên: 21/02/2014, 03:20