Báo cáo khoa học: Alternative binding proteins: Anticalins – harnessing the structural plasticity of the lipocalin ligand pocket to engineer novel binding activities pdf
Báo cáo khoa học: Alternative binding proteins: Anticalins – harnessing the structural plasticity of the lipocalin ligand pocket to engineer novel binding activities pdf
... MINIREVIEW Alternative binding proteins: Anticalins – harnessing the structural plasticity of the lipocalin ligand pocket to engineer novel binding activities Arne SkerraLehrstuhl ... framework of the pairedvariable domains of the light and heavy chains. TheseCDRs come together at the tips of the Y-shaped mole-cule to form a contiguous interface for antigen bind-ing. On the ... bound at the bottom of the cleft thatharbours biliverdin IXcin the BBP [24]. Thus, while the overall topology of the lipocalin, comprising the b-barrel with the a-helix attached to it, remained...
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Tài liệu Báo cáo khoa học: Verprolin function in endocytosis and actin organization Roles of the Las17p (yeast WASP)-binding domain and a novel C-terminal actin-binding domain doc
... Loss of the LBD abolished the ability of C-Vrp1p364)817 to restore growth of vrp1D cells at elevated temperature.We next assessed the importance of the LBD for the ability of C-Vrp1p364)817 to ... in yeast [55]. Binding to WIP serves to protect WASP fromdegradation [5 5–5 7]. In another study, WIP binding inhibited the ability of the WASP-family protein(N-WASP) to activate the Arp2 ⁄ 3 complex ... altering the mitochondrial–cytoplasmic distribution of Mod5pimplicate the actin cytoskeleton and mRNA 3¢ endsand ⁄ or protein synthesis in mitochondrial delivery. MolCell Biol 15, 688 4–6 894.19...
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Báo cáo khoa học: Relation between domain evolution, specificity, and taxonomy of the a-amylase family members containing a C-terminal starch-binding domain pot
... structure [ 2–9 ,1 1–1 5,3 8–4 1,9 5–9 9,111],although the length of this domain is variable within the family. The final partial tree, the ÔSBD treeÕ, lacks the character of a tree consisting of two groups, ... Withregard to the Nostoc CGTase, it matches best the a-amylasefrom Streptomyces griseus, a representative of the a-amy-lases produced by streptomycetes. The positioning of aNostoc CGTase in the assumed ... separate evolution of this domain [30].This together with the findings of the present study indicatesa separate evolution of the domains C and E compared to the domains A and B. The recent introduction...
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Báo cáo khoa học: b-Secretase cleavage is not required for generation of the intracellular C-terminal domain of the amyloid precursor family of proteins pot
... control)02550751001251489864+ – KO WT Tg – +22C111489864Aβ rodent+ – KO WT Tg – +E1489864C8+ – KO WT Tg – +F36anti-GAPDH+ – KO WT Tg – +APPsα total (% of control)Fig. 1. Levels of total APPs are ... translate intoincreased amounts of APP or APLP2 CTFs. Therefore,it is reasonable to suppose that the levels of CTFs areregulated, possibly by degradation of excess CTFs.Together, these results ... translocates to the nucleus in a notch-like manner. J Biol Chem 276,4028 8–4 0292.64 Laemmli UK (1970) Cleavage of structural proteinsduring the assembly of the head of bacteriophage T4.Nature 227, 68 0–6 85.Supporting...
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Tài liệu Báo cáo khoa học: Toggle switches, pulses and oscillations are intrinsic properties of the Src activation/deactivation cycle doc
... of the period and amplitude of Src oscillations by the activities of the activatory phosphatase RPTP and inhibitorykinase Csk. Dependence of the oscillation amplitude (A) and period(B) on the ... presenting the pulse of Src activity in the plane of the inactive and active fractions, siand s1. If the duration of the stimulus exceeds the critical value, the trajectory in the (si, ... found byequating the determinant of the Jacobian matrix of Eqns( 6–8 ) to zero (fold bifurcation). The Hopf bifurcation curveis determined by equating the sum of the two eigenvalues to zero and taking...
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Tài liệu Báo cáo khoa học: A facile method for expression and purification of the Alzheimer’s disease-associated amyloid b-peptide pdf
... the peptide is bound in a concentrated mannerat the top of the column, or, if bound to the resinprior to packing the column, the salt gradient concen-trates the peptide on its way out of the ... Ab( 1–4 0) and Ab( 1–4 2)coding sequences together with added ATG codons were cloned directlyinto a Pet vector to facilitate production of Met-Ab( 1–4 0) and Met-Ab( 1– 42), referred to as Ab(L 1–4 0) ... A common drawback of such approachesis the cost of the affinity resins used to isolate the fusion protein and the proteases required to liberate the protein of interest from the fusion protein....
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Tài liệu Báo cáo khóa học: Active-site residues and amino acid specificity of the bacterial 4¢-phosphopantothenoylcysteine synthetase CoaB pptx
... hasFig. 5. Analysis of the enzymatic activity of His-CoaB R206Q and A276V. The synthesis of 4¢-phosphopantothenoyl-CMP (–cysteine, upper part of the figure) and of 4¢-phosphopantothenoylcysteine (+ ... identifiedand shown to be involved in the second half reaction of the (R)-4¢-phospho-N-pantothenoylcysteine synthetase. The proposed intermediate of the (R)-4¢-phospho-N-panto-thenoylcysteine synthetase ... formation of 4¢-phosphopantothenoyl-CMP (activation of the carboxyl group of 4¢-phosphopantothenate). In a second step, the amide bond of PPC (shaded in grey) is formed by reaction of 4¢-phosphopantothenoyl-CMP...
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Tài liệu Báo cáo khoa học: Endogenous expression and protein kinase A-dependent phosphorylation of the guanine nucleotide exchange factor Ras-GRF1 in human embryonic kidney 293 cells pptx
... using the SV total RNA isolationsystem (Promega, Madison, WI, USA), according to the manufacturer’s protocol. Paramagnetic beads were used to isolate mRNA from the pool of total RNA. The purifiedmRNA ... another major GEF withactivity towards Ras. Ras-GRF1 mediates activation of Ras subsequent to the stimulation of Gi- and Gq-coupled receptors [8,9]. The main mechanism for the activation of ... higherlevel than the 140 kDa isoform. The serotonin-inducedincrease in phosphorylation of both isoforms wasreduced by the coexpression of hPDE4D2 with 5-HT7receptors (Fig. 3C). This was also the case...
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Tài liệu Báo cáo khoa học: "Exploiting Readymades in Linguistic Creativity: A System Demonstration of the Jigsaw Bard" docx
... the scale of the Bard’s genera-tivity, and the quality of its insights.5 Empirical Evaluation The vastness of the web, captured in the large-scale sample that is the Google ngrams, means the Jigsaw ... can one make playful – and creative – allusions to the writings of others, or14mischievously subvert the conventional wisdom of platitudes and clichés? Orwell’s use of the termreadymade is ... the public’s appre-ciation of artistic creativity – despite Duchamp’sFountain being considered one of the most influ-ential artworks of the 20th century – we shall showthat the notion of...
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Tài liệu Báo cáo khoa học: Cell surface heparan sulfate proteoglycans Target and partners of the basic ®broblast growth factor in rat Sertoli cells pptx
... hows that the b FGF r egulation of their expression speci®cally dependson the nature of HSPG and of the Sertoli cell developmentalstage.In conclusion, HSPG are partners and the target of bFGFin ... for the recognition of the speci®c bFGFreceptor, leading to the formation of a ternary complexcomprising HSPG±bFGF±FGFR. These oligosaccharidicmotifs are differently sulfated related to the ... 20-day-old-rat Sertoli cells.During testicular development, the physiology of Sertolicells i s m odi®ed. The cell proliferation decreases and ceasesallowing the establishment of the hematotesticular...
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