Báo cáo khoa học: AKAP12 in astrocytes induces barrier funct

Báo cáo khoa học: AKAP12 in astrocytes induces barrier functions in human endothelial cells through protein kinase Cf pptx

... compilation ª 2008 FEBS 2353 AKAP12 in astrocytes induces barrier functions in human endothelial cells through protein kinase Cf Yoon Kyung Choi and Kyu-Won Kim NeuroVascular Coordination Research Center, ... as A -kinase anchor protein 12 (AKAP12) [11]. AKAP12 acts as a multivalent scaffold protein, and has been shown to associate with protein kinase C (...

Ngày tải lên : 23/03/2014, 07:20

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Báo cáo khoa học: Identiﬁcation of the heparin-binding domains of the interferon-induced protein kinase, PKR pptx

... and heparin-binding residues are present within the amino- terminal heparin-binding domain (ATD) of PKR. Thus, in contrast to the dsRNA-binding domain of PKR, its heparin-binding domains are located in ... minor role in heparin binding (Fig. 2). However, when combined with the DM, it resulted in 86% loss of heparin binding. As the dsRNA–binding domain does not overlap with PKR’s hep...

Ngày tải lên : 23/03/2014, 13:20

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Báo cáo khoa học: Exogenous oxidative stress induces Ca2+ release in the yeast Saccharomyces cerevisiae potx

... seconds). The cells were resuspended in 0.1 m Mes ⁄ Tris buffer (pH 6.5) and transferred to the luminometer tube. A cellular luminescence baseline was determined by 1 min of recording at 1 s intervals. ... Ca 2+ transport in Saccharomyces cerevisiae. J Exp Biol 196, 157–166. 11 Cunningham KW & Fink GR (1996) Calcineurin inhib- its VCX1-dependent H + ⁄ Ca 2+ exchange and induces Ca...

Ngày tải lên : 15/03/2014, 23:20

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Báo cáo khoa học: Gene duplication and separation of functions in aB-crystallin from zebraﬁsh (Danio rerio) pptx

... also aid in protein refolding [6]. The mechanism behind the chaperone-like activity of a-crystallin is of great interest because protein aggregation is believed to play a prominent role in the ... cataracts, a leading cause of human blindness. Temperature has a large in uence on the ability of a-crystallin to inhibit protein aggregation. For example, raising incubation temperat...

Ngày tải lên : 30/03/2014, 11:20

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Tài liệu Báo cáo khoa học: Impact of the native-state stability of human lysozyme variants on protein secretion by Pichia pastoris doc

... character is maximal in early folding intermediates. Protein Sci 4, 1553–1562. 32 Cereghino GP, Cereghino JL, Ilgen C & Cregg JM (2002) Production of recombinant proteins in fermenter cultures ... plasmid containing the cDNA sequence encoding mature WT human lysozyme was constructed according to the supplier’s instructions (Invitrogen). In order to direct the expressed protein...

Ngày tải lên : 19/02/2014, 08:20

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Báo cáo khoa học: The speciﬁc delivery of proteins to human liver cells by engineered bio-nanocapsules doc

... various proteins for the C-terminal moiety of the L fusion proteins, depending on the character of the proteins to be fused. This possibility will include cytoplasmic proteins, as well as cytokines ... large (L) proteins [1]. In 1982, Valenzuela et al. succeeded in preparing recombinant sAg from yeast as S protein particles with a diameter of 22 nm [2]. Around 1990, recombinant L protei...

Ngày tải lên : 23/03/2014, 15:20

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Báo cáo khoa học: Oligomerization states of the association domain and the holoenyzme of Ca2+ ⁄CaM kinase II pptx

... adjacent kinase domains impede the binding of Ca 2+ ⁄ CaM. These interactions may induce a strain in the ring that is released in the absence of the kinase domains allow- ing the association domains ... relaxing from a 6-fold ring into a more stable 7-fold form. Implicit in this idea is that the N-terminal kinase domains interact with each other around the ring of the association...

Ngày tải lên : 30/03/2014, 11:20

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Tài liệu Báo cáo khoa học: Interaction between very-KIND Ras guanine exchange factor and microtubule-associated protein 2, and its role in dendrite growth – structure and function of the second kinase noncatalytic C-lobe domain docx

... February 2011) doi:10.1111/j.1742-4658.2011.08085.x The kinase noncatalytic C-lobe domain (KIND) is a putative protein protein interaction module. Four KIND-containing proteins, Spir-2 (actin-nuclear factor), PTPN13 (protein tyrosine phosphatase), ... with GST-CD2 (MAP2 v-KIND binding core). Input (cerebellum) GST v-KIND KIND1 v-KIND KIND2 Spir-2 KIND PTPN13 KIND Input (MAP2) GST v-KIND...

Ngày tải lên : 14/02/2014, 19:20

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Tài liệu Báo cáo khoa học: Roles of AP-2 transcription factors in the regulation of cartilage and skeletal development doc

... activity by protein kinase A-induced phosphorylation of the transcription factor [29]. So far, interactions with AP-2 have been described for many proteins. For example, CBP ⁄ p300-interacting transactivator ... transcriptional activation in response to two different signal transduction pathways, the phorbol ester-activated protein kinase C pathway, or the cAMP- dependent protein...

Ngày tải lên : 16/02/2014, 09:20

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Tài liệu Báo cáo khoa học: Molecular defect of isovaleryl-CoA dehydrogenase in the skunk mutant of silkworm, Bombyx mori ppt

... recombinant BmIVD protein Monolayers of Sf9 cells in a 150-mm dish were infected with BmIVD-recombinant AcMNPV. After 72 h, the culture med- ium was discarded and cells were suspended in 10 mL NaCl ... forms the binding pocket for the branched-chain acyl moiety of substrate isovaleryl- CoA in human IVD [7,8]. As represented in Fig. 2C, both residues were conserved in silkwor...

Ngày tải lên : 18/02/2014, 04:20

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