Báo cáo khoa học: Investigation of the interaction between the atypical agonist c[YpwFG] and MOR docx

... site. The mutation also affects the apparent K m of ATP, indicating that it modifies the active site of the enzyme to alter the affinity for ATP. The R1441C and Y1699C mutations within the Roc and ... increases the specific activity of LRRK2, and also alters its apparent K m for ATP. The G2019S residue lies within the activation loop of the kinase, and poten...

Ngày tải lên: 23/03/2014, 06:20

... al. The atypical opioid agonist c[YpwFG] FEBS Journal 275 (2008) 2315–2337 ª 2008 The Authors Journal compilation ª 2008 FEBS 2333 Investigation of the interaction between the atypical agonist c[YpwFG] ... optimization of the com- plexes [33,34], providing a more detailed description of the binding mode and the electronic and steric proper- ties of...

Ngày tải lên: 23/03/2014, 07:20

... 115, and 119. The model of this region as an a-h elix places Trp125 two turns of the helix above His119 and not on the same face o f the helix. Therefore, the distance and the orientation of His119 ... [26] and the gating of the proton channel [27,28] can be over come by the addition of arachidonate acid. The site of interaction with and the mecha...

Ngày tải lên: 07/03/2014, 16:20

... exhibit the same kinetics as those located on the intact protein. Therefore, determination of the kinetics of phosphorylation of these peptides allows the order of phosphorylation of the six ... structure. The two groups of peaks at 6.85 and 7.15 p.p.m. correspond to the aromatic resonances of the seven tyrosines in the protein, and the inset 2D expansion...

Ngày tải lên: 08/03/2014, 02:20

... enzyme active sites and groups of the reactant transition state (S à )[2] ;the strength of the n oncovalent interaction between enzyme active sites and S à determines the stability of the enzyme– transition ... complex and the rate of the reaction. Thus, in theory, modifications of the enzymatic specificity could be accomplished by changing a ctive-site residu...

Ngày tải lên: 16/03/2014, 18:20

... for the natural abundance signal were made. In the case of the aspartate peaks, the amount of 13 C was estimated by use of the integrals and the known dioxane concentration. The contributions of ... addition of 100 mm unlabelled amino acid. The amount of 13 C in each resonance was evaluated by integration of the extract peaks and the corresponding peaks i...

Ngày tải lên: 30/03/2014, 10:20

... 5B). The ER is the site of synthesis of all secretory pro- teins and resident proteins of the membrane system, and any perturbation that compromises the protein- folding capacity of the organelle ... to the intensity and the duration of the stress. An initial adaptive response culminates in the temporary inhibition of protein synthesis, providing cells with...

Ngày tải lên: 14/02/2014, 22:20

... the activity of this SE-synthesiz- ing enzyme. Considering the importance of sterols for cell polarization, and the interactions between PAKs and proteins catalyzing sterol synthesis and storage, ... Taken together, these observations suggest that sterol synthe- sis may play a crucial role in cell polarization and in the function of PAKs and sterol biosynthetic prote...

Ngày tải lên: 18/02/2014, 13:20

... Frequency of Request types Since most of each dialogue consisted of the making of requests, the first analysis examined the frequency of the various kinds of requests in the corpus of five ... National Institute of Education under contract US-NIE-C-400-76-0116 to the Center for the Study of Reading of the University of Illinois and Bolt, Beranek a...

Ngày tải lên: 21/02/2014, 20:20

... lipid-A and two phosphate moieties. The ions with m ⁄ z of 1519.6 and 1541.6 are mono- and di-sodiated forms of the de-O-acylated LOS and the ion with m ⁄ z of 1479.6 is the anhydrous form of the ... moieties. The de- O-acylated lipid-A unit consists of two GlcNAc and two 3-OH C12:0 groups. Among the other ions m ⁄ z of 2654.8, 2694.8 and 2717.4 indicates...

Ngày tải lên: 07/03/2014, 05:20