Báo cáo khoa học: Lipid-induced conformational transition of the amyloid core fragment Ab(28–35) and its A30G and A30I mutants docx

... amyloid core fragment Ab(28–35) and two of its mutants, A30G and A30I, to understand the peptide–membrane interactions, and especially the contributions of the hydrophobic resi- dues Ala, Gly and ... that the binding of the amyloid protein of AD to lipid membranes can change their fluidity [34]. Therefore, we examined the effect of bind- ing of...

Ngày tải lên: 23/03/2014, 07:20

... with the metal ion abolished. Dynamics and energetics of the conformational transitions The DG | values for the transition between state 1 and 2 in complexes of wild-type protein with GppNHp and GppCH 2 p ... states 1a and 1b. The trans- ition velocity between these two states and thus the energy of the transition state is similar to that found for transit...

Ngày tải lên: 30/03/2014, 09:20

... conformational stability of HDAH, DDG u , upon inhibitor binding is the difference between the free energy of unfolding of HDAH in the absence of inhibitor and in the presence of 100 l M of the ... refolding of HDAH The crystal structure of HDAH contains one Zn 2+ ion at the bottom of the active site and two K + ions in the neighbourhood of the ac...

Ngày tải lên: 30/03/2014, 08:20

... at the flexible regions of an otherwise folded polypeptide chain. CD and NMR studies of the CII protein have indicated the presence of a rigid core and flexible region(s) within the molecule. These ... the presence of GdnHCl, both in the down- field (amide) region (Fig. 8A) and the up-field region (Ca and sidechain, Fig. 8B). This indicates the absence of any g...

Ngày tải lên: 30/03/2014, 20:20

... presence of alternative splicing around the 5¢-end of exon 6 of Meis2 and Meis3 was tested by RT-PCR. The positions of molecular mass markers are shown to the left, and the size in base pairs of the ... However, the activity of the full-length Meis1a was not tested, and on the basis of our work we expect that its activity would be inhibited by the conse...

Ngày tải lên: 16/02/2014, 15:20

... obtained with the transition at posi- tions 1915 and 1917. These mutations had an effect on the formation of pseudouridine exclusively at the mutant position and did not affect either of the other two ... of the wild-type 23S rRNA from the 50S and the 70S particles were similar: stop sites were found at positions 1911 and 1917 on the CMCT treated RNA and not...

Ngày tải lên: 18/02/2014, 16:20

... conjunctively the phenol-hydroxyl group of BPA. Fig. 1. Chemical structure BPA and its ball -and- stick structure, together with a space-filling structure in the ligand-binding pocket of the ERRc. The space-filling ... shown). The K D values were 23.4 nm (approximately 25% of the binding affin- ity for the wild-type ERRc) (Table 1). The IC 50 values of BPA and 4-OHT...

Ngày tải lên: 18/02/2014, 16:20

... to the right and the addition of further residues at the N-terminus or beyond residue 34 of the inhibitory segment is superfluous. However, in the case of PpPr, the serendipitous positioning of ... each extremity of the polypeptide chain, must of necessity arise from contacts that are made outwith the active site of the enzyme. The effect of removal of...

Ngày tải lên: 19/02/2014, 00:20

... less sensitive to the pres- ence of the anion. These residues also modulate the conformation of unfol- ded cytochrome c, influencing its spin state and the coordination to the prosthetic group. Abbreviation CT, ... ligand X (the other likely candidates, i.e. the lysines, are fully proto- nated at pH 2.2) [18]. The heterogeneous character of the A-state promp- ted us...

Ngày tải lên: 19/02/2014, 05:20

... the SP isoforms when the con- centrations of these isoforms was standardized to 1.0 mm. Significantly, the TH activity of the SP isoforms showed concentration dependence in the presence of 0.2 mm ... the relationship between TH activity and the isoforms, especially the existence of a co-operative effect of the isoforms in the QAE and SP groups. We recently fou...

Ngày tải lên: 19/02/2014, 16:20