Báo cáo khoa học: Crystal structure of a cold-adapted class

Tài liệu Báo cáo khoa học: Crystal structure of a subtilisin-like serine proteinase from a psychrotrophic Vibrio species reveals structural aspects of cold adaptation docx

... I, Moe E, Lanes O, Smalas AO & Willassen NP (2003) The structure of uracil–DNA glycosylase from Atlantic cod (Gadus morhua) reveals cold-adaptation features. Acta Crystallogr Sect D 59, 1357–1365. 10 ... CA, USA]. Acknowledgements We thank Andrea Schmidt for assistance during data collection at DESY beamline BW 7A and Achim Dick- manns for comments on the manuscript. References 1 Blo...

Ngày tải lên : 19/02/2014, 16:20

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Báo cáo khoa học: Crystal structure of a glycoside hydrolase family 6 enzyme, CcCel6C, a cellulase constitutively produced by Coprinopsis cinerea pot

... A ˚ (CcCel 6C HinCel 6A, 1BVW), 1.14 A ˚ (CcCel 6C HjeCel 6A, 1QK0 chain A) and 1.30 A ˚ (CcCel 6C HinCel6B, 1DYS chain A) for main chain atoms. The signiﬁcant feature in cellobiohydrolases HjeCel 6A and ... are illustrated as stick models. (C) Comparison of the Ca backbones of unliganded CcCel 6C (blue), CcCel 6C pNPG3 (green) and CcCel 6C cellobiose (red). Y. Liu et al. Str...

Ngày tải lên : 15/03/2014, 10:20

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Báo cáo khoa học: Crystal structure of a cold-adapted class C b-lactamase potx

... transpeptidase. b-Lactamases of Keywords class C b-lactamase; cold adaptation; psychrophile; weak interactions; X-ray structure Correspondence C. Michaux, Chimie Biologique Structurale Laboratory, CPTS ... the structure of the class C b-lactamase from E. cloacae P99 (Protein Data Bank code 2BLT) as a search model. The model was solved to a resolution of 2.2 A ˚ ....

Ngày tải lên : 23/03/2014, 07:20

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Báo cáo khoa học: Crystal structure of a staphylokinase variant A model for reduced antigenicity pptx

... using a- cyano-4-hydroxycinnamic acid (CCA) as the matrix. Protein crystallization Crystallization trials were carried out using the hanging- drop vapor-diffusion method at 293 K. Crystals were obtained a few days after ... dimer interface. The total buried surface areas of the a a or head± tail geometries were more than 1000 A Ê 2 , m uch larger than the a verage buried surface ar...

Ngày tải lên : 23/03/2014, 21:21

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Báo cáo khoa học: Crystal structure of a designed tetratricopeptide repeat module in complex with its peptide ligand pot

... com- posed of three TPR repeats (AB-helix pair) and an additional C- terminal capping helix (A cap ). The only way for molecules AB–AB–AB A cap to arrange on ‘head-to-tail’ packing is if the C- terminal ... Hsp90-speci c residues ‘grafted’ onto the binding surface of CTPR3 [5]. The pairwise backbone align- ment of CTPR3 (Protein Data Bank ID: 1Na0) and A B Fig. 2. Crystal packi...

Ngày tải lên : 29/03/2014, 08:20

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Tài liệu Báo cáo khoa học: Crystal structure of importin-a bound to a peptide bearing the nuclear localisation signal from chloride intracellular channel protein 4 ppt

... digital abstract l CLIC4 and importin alpha bind by x-ray crystallography (View interaction) Abbreviations ARM, armadillo; CLIC, chloride intracellular channel; NLS, nuclear localization signal; ... integration of macromolecular diffraction data. Acta Crystallogr D: Biol Crystallogr 62, 48–57. 36 Collaborative Computational Project Number 4 (1994) The CCP4 suite: programs for protein crysta...

Ngày tải lên : 14/02/2014, 19:20

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Tài liệu Báo cáo khoa học: Crystal structure of Klebsiella sp. ASR1 phytase suggests substrate binding to a preformed active site that meets the requirements of a plant rhizosphere enzyme doc

... Holst MJ & McCammon JA (2001) Electrostatics of nanosystems: Applications to microtubules and the ribosome. Proc Natl Acad Sci USA 98, 10037–10041. 39 Wallace AC, Laskowski RA & Thornton ... 6-phytases. However, the crystal structure of E. coli AppA shows phytate bound with the 3-phosphate as a scissile group, although its biochemical characterization classi- ﬁes it as a 6...

Ngày tải lên : 16/02/2014, 09:20

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Tài liệu Báo cáo khoa học: Crystal structure of the catalytic domain of DESC1, a new member of the type II transmembrane serine proteinase family pptx

... expression analysis between squamous cell carcinoma of the head and neck and normal tissue led to the identiﬁcation of a differential expressed squamous cell carcinoma gene 1 (DESC1). The data indicated ... precursor by human colorectal carcinoma cells and its modulation by cytokines ⁄ growth factors and proteinase inhibitors. Biol Chem 380, 473–483. 17 Kataoka H, Uchino H, Asada Y, Hat...

Ngày tải lên : 19/02/2014, 00:20

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Tài liệu Báo cáo khoa học: Crystal structure of the BcZBP, a zinc-binding protein from Bacillus cereus doc

... hyper- thermophilic archaeon Thermococcus kodakaraensis KOD1. J Bacteriol 185, 5175–5180. 14 Tanaka T, Fukui T, Fujiwara S, Atomi H & Imanaka T (2004) Concerted action of diacetylchitobiose deacetylase and ... from Table 1. Deacetylase activity of the BcZBP protein on N-acetylchi- tooligosaccharides. Substrate Deacetylation (%) GlcNAc 100 (GlcNAc) 2 89.82 (GlcNAc) 3 10.51 (GlcNAc) 4...

Ngày tải lên : 19/02/2014, 00:20

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Báo cáo khoa học: Crystal structure of basic 7S globulin, a xyloglucanspeciﬁc endo-b-1,4-glucanase inhibitor protein-like protein from soybean lacking inhibitory activity against endo-b-glucanase doc

... DASA of the AB dimer is deﬁned as [(ASA of A) + (ASA of B) ) (ASA of AB dimer)] ⁄ 2. The number of water molecules in the dimer interface was detected with ASV CALCULATOR [34]. ASA was calculated ... Kinoshita S, Tomatsuri H, Umeda K, Ueda S, Hata Y & Arai M (1999) Analysis of a catalytic acidic pair in the active center of cellulase from Aspergillus aculeatus. Biosci B...

Ngày tải lên : 14/03/2014, 23:20

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