Báo cáo khoa học: Characterization of thermostable aminoacylase from hyperthermophilic archaeon Pyrococcus horikoshii pot

... plasmids. Isolation of the IDH gene ( idh ) from C. limicola Construction of a genomic DNA library of C. limicola M1 has been described previously [24]. A partial DNA fragment of idh was amplified from C. ... function of IDH kinase/phosphatase, that leads to inactivation of the enzyme and consequent switch of the carbon flux from TCA cycle to glyoxylate cycle [13] [14]. Ther...

Ngày tải lên: 08/03/2014, 10:20

... both rounds of PCR. In a third round of PCR, the PCR products from round two were, in the case of A. thali- ana SCP-2, amplified with primers SCPAtCN and SCPAtNE, or, in the case of E. lagascae ... substrate for SCP-2, it would result in a decrease in the rate of transfer of BODIPY-PC because of the competition from the presence of a new distinct transferable lipid pool. Wh...

Ngày tải lên: 16/03/2014, 12:20

... the generation of Ab peptides in pathological processes. Study of the characteristics of the PS complex will facilitate elaboration of the molecular processes involved in the production of Ab. Analysis of ... and includes lack of Ab amyloid peptide generation and Notch signalling in PS double knockout cells, cofractionation of activity with PS, abolition of activity with mut...

Ngày tải lên: 16/03/2014, 16:20

... Academy of Sciences, Szeged, Hungary 4 Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Debrecen, Hungary 5 Department of Pharmaceutical Chemistry, University of ... activation time (s) and the maximum rate (v max ) of sub- strate hydrolysis ( kE) from our data. From the length of the lag-phase (s), the rate constant of activation (k a...

Ngày tải lên: 16/03/2014, 02:20

... 10 6 mL )1 . Next, 0.5 mL of propidium iodide staining solution (10 mg of propidium iodide, 0.1 mL of Triton-X, 3.7 mg of EDTA in 100 mL of NaCl ⁄ P i ) was added to 0.5 mL of cell suspension. The ... spectra of extracted lipids (A) and PCA extracts (A¢) from one representative sample of MCF-7 cells; 2D 1 H NMR spectra of extracted lipids (B) and PCA extracts (B¢) from o...

Ngày tải lên: 18/02/2014, 13:20

... con- servation of this class of proteins at both the structural and functional levels. Given the multiplicity of CLPs in humans and insects [14], we hypothesized that homologues of the previously ... gigas. In this article, we report the characterization of a new CLP, named Cg-Clp2, from the oyster C. gigas. The tissue distribution and temporal pattern of expres- sion of the...

Ngày tải lên: 19/02/2014, 00:20

... number of transcripts containing exon 1a with the number starting from exon 1. The abun- dance of transcripts starting from exon 1 was 10-fold higher than that of the transcripts starting from exon ... 5¢-flanking region of exon 1 in DNASE1. Deletion of the upstream end of the 5¢-flanking region of exon 1 from position )1386 to )231, )116, or )78 did not result in any sign...

Ngày tải lên: 19/02/2014, 06:20

... are from one experiment, representative of three giving similar results. Fig. 6. Dependence on the concentration of PPI1 588 His 6 of the sti- mulation of H + -ATPase activity in PM purified from ... the mechanism of action of PPI1. The analysis of the pH dependence of PPI1- induced activation of the H + -ATPase showed that stimulation decreases dramatically with the incr...

Ngày tải lên: 19/02/2014, 07:20

... Proteinase K from Tritirachium album Limber. Characterization of the chromosomal gene and expression of the cDNA in Escherichia coli. Eur J Biochem 179, 185–194. Characterization of a Serratia ... Isolation of genomic DNA from Serratia sp. Genomic DNA was isolated as described by Chen and Kuo [38] for use in identification of the peptidase gene. Generation of an  200-bp fr...

Ngày tải lên: 19/02/2014, 07:20

... of all of these genes remains to be clarified. Expression and purification of Sno1p and Snz1p In light of the similarity in the amino acid sequence of Sno1p to that of the glutaminase subunit of ... absorbance of the supernatant was read at 363 nm. The absorbance of the reduced form of APAD (APADH) was linear over the 2–100 l M range of glutamate with a molar extinction co...

Ngày tải lên: 19/02/2014, 12:20