Báo cáo khoa học: Alpha 1-antichymotrypsin/SerpinA3 is a novel target of orphan nuclear receptor Nur77 potx

... pcDNA3.1-GST -Nur77 plasmid. pSilencer-shNur77 was prepared by overlappingstrategy with primers 5¢-gacGGATCCgcagtccagccatgctcctttcaagagaaggagcatg-3¢ (with BamHI), 5¢-cggAAGCTTtATCGATccaaaaaacagtccagccatgctccttctcttg-3¢ ... 5¢-ATGGTGGGAATGGGTCAGAAG-3¢ and 5¢-CACGCAGCTCATTGTAGAAGG-3¢. Another primer pairused for SerpinA3 was ACTas5-ACTas3: 5¢-GAATCCACCAGCTACATCCA-3¢ and 5¢-GTGCCCTCCTCAAATACATCA-3¢.Western blot assayThe cells ... 5¢-CTAATCTCTTCCTCCAAAAAGCACACAGA-3¢ for St-182 and St-93/-182, 5¢-AGAAATTATCATCTTTTCCAGTCCGAGA-3¢for St-93 and St-93/-182, and 5¢-TGGTCTTGAACTCCTCGTGATCTGCCCA-3¢ for Lst-595.pcDNA3.1 -Nur77 expression plasmid...

Báo cáo khoa học: Xenopus Rbm9 is a novel interactor of XGld2 in the cytoplasmic polyadenylation complex pptx

... RNA helicase in translational repression is anRNA-mediated oligomer. Nucleic Acids Res 32, 1325–1334.5 Nakahata S, Kotani T, Mita K, Kawasaki T, Katsu Y,Nagahama Y & Yamashita M (2003) ... suggesting anacceleration of the G2 ⁄ M transition in meiosis I. Thishastening of maturation was correlated with a preco-cious synthesis of Mos and AuroraA proteins, andwith the activation of the ... Kumagai S,Watanabe H, Yamashita M, Kashiwabara S, Miyado K& Baba T (2006) Possible role of mouse poly (A) polymerase mGLD-2 during oocyte maturation. DevBiol 289, 115–126.19 Rouhana L...

Tài liệu Báo cáo khoa học: KIPase activity is a novel caspase-like activity associated with cell proliferation doc

... substrate for caspase 1;Ac-IETD-AMC is a substrate for caspase 8 and 10;Ac-LEHD-AMC is a substrate for caspases 2, 4, 5 and 9.Ac-DVPD-AMC, Ac-DPSD-AMC and Ac-ESQD-AMCare tetra peptide substrates representing ... follows: caspase 1, WEHD-AMC; caspase 2, VDVAD-AMC; caspase 3, DEVD-AMC; caspase 4, WEHD-AMC; caspase 5,WEHD-AMC, caspase 6, VEID-AMC; caspase 7, DEVD-AMC; ca-spase 8, IETD-AMC; caspase 9, LEHD-AMC; ... Interestingly, a distantrelative of the caspases, termed paracaspase, was identiﬁedin Homo sapiens using a PSI-BLASTsearch [39]. Paracaspasecould be a candidate gene for KIPase although no caspaseactivity...

Báo cáo khoa học: Intermittent hypoxia is a key regulator of cancer cell and endothelial cell interplay in tumours pot

... Mirandola P, Gessi S, Varani K,Leung E, Maclennan S, Baraldi PG & Borea PA(2007) Hypoxia inhibits paclitaxel-induced apoptosisthrough adenosine-mediated phosphorylation of bad inglioblastoma ... enzymes that lead to extracellular matrix degra-dation (matrix metalloproteases) [75–78]. In addition,NF-jB activation was reported as an early event inmalignant transformation in vitro [79], and ... Rogers AE et al. (2000) Activation of NF-kap-paB ⁄ Rel occurs early during neoplastic transformation of mammary cells. Carcinogenesis 21, 871–879.80 Rayet B & Gelinas C (1999) Aberrant rel...

Tài liệu Báo cáo khoa học: Human Cdc45 is a proliferation-associated antigen pdf

... cellular proliferation in histological material is a valuable component of conventional histopathologi-cal analysis and may be of major prognostic import-ance [56]. Proliferation in immunohistochemicalsections ... immunoreactivity of a Cdc45-speciﬁc antibody on formalin-ﬁxed andparafﬁn-embedded tissues (Fig. 9). Antibodies againstPCNA and Cdc45 stained malignant cells in a compar-able manner, e.g. on invasive-lobular ... 4b-phorbol 12-myristate 13-acetate (PMA). After24 h of PMA incubation HL60 cells showed severalmonocyte ⁄ macrophage-like characteristics, such as anintense clustered adherence of almost all cells...

Tài liệu Báo cáo khoa học: Hu-K4 is a ubiquitously expressed type 2 transmembrane protein associated with the endoplasmic reticulum ppt

... putative polyadenylation signals(AATAAG and AATAAC) are present about 20nucleotides in front of the poly (A) tail (Fig. 3A) . Bothare not identical to the most often used signal AATAAA which is ... 4400044013CCA ATG A CGC ATG CWeakWeak>54aa9aa1¢ 44 68% –2 > 53 20% 55046 TAT ATG T Weak 3 aa2 ⁄ 4 55067 TCA ATG C Weak 58 aa3 33 39% 609296093360939GTA ATG CTGC ATG TTCC ATG GGAdequateWeakAdequate13 ... the plasma membrane or with themembranes of intracellular organelles although theylack a transmembrane domain. They are attached tothe cytoplasmic face of the membranes via palmitoylanchors...

Báo cáo khoa học: Syndecan-4 is a signaling molecule for stromal cell-derived factor-1 (SDF-1)/ CXCL12 pptx

... stromalcell-derived factor (SDF) - 1alpha. Glycobiology 10, 21–29.34 Valenzuela-Fernandez A, Palanche T, Amara A, Mage-rus A, Altmeyer R, Delaunay T, Virelizier JL, BaleuxF, Galzi JL & Arenzana-Seisdedos ... migrate as follows: SD-4as a 100–250 kDa broad smear, SD-1 as a single98 kDa band, CD44 as a 110 kDa band, SD-2 as a 50 kDa protein. Beta-glycan migrates as two broadbands of 55 and 100 kDa, respectively ... byT-cell-line-adapted HIV-1. Nature 382, 833–835.7 Pablos JL, Amara A, Bouloc A, Santiago B, Caruz A, Galindo M, Delaunay T, Virelizier JL & Arenzana-Seis-dedos F (1999) Stromal-derived factor is...

Báo cáo khoa học: Cytochrome b6f is a dimeric protochlorophyll a binding complex in etioplasts doc

... andferredoxin-NADP+-reductase bands, and at k =554 nm for the Cyt f a- band (Fig. 3). However, theabsorbance maximum at k = 668 nm characteristicfor Chl was lacking in etioplasts, whereas a peak atk ... indicating a structural or a functional role for bothpigments. A structural role was indicated in a Chl-less mutantthat was reported to lack accumulation of theCyt b6f complex in Clamydomonas ... pro- and eukaryotic organisms [19,20]and spectra showing an absorbance maximum at670 nm [4,5,7,10]. By contrast, the dimeric Cyt b6fcomplex of etioplasts exhibited an absorbance maxi-mum at...

Báo cáo khoa học: Bacterial IscU is a well folded and functional single domain protein pdf

... Bacterial IscU is a well folded and functional single domain proteinSalvatore Adinolﬁ1, Francesca Rizzo1, Laura Masino1, Margie Nair1, Stephen R. Martin1, Annalisa Pastore1and ... (A) 1H-15NHSQCspectrum of uniformly15NlabeledsampleofE. coli IscU; (B) as forsample A, after addition of Fe(II) in a Fe(II)/IscU ratio of 2 : 1;(C) same as sample A with a ratio Fe(II)/IscU of 5 : 1 after partialoxidation ... here a structural characterization of thisprotein and demonstrate that E. coli IscU can be obtainedas a recombinant well-folded protein. We demonstrate thatour construct can function as a scaffold...

Tài liệu Báo cáo khoa học: ˚ The 1.8 A crystal structure of a proteinase K-like enzyme from a psychrotroph Serratia species docx

... of Asp11, Asp14 and Asp21, the amideoxygen atom of Gln15 and the carbonyl oxygen atoms of Asp11 and Asn23 (Fig. 3) in an arrangement similarto what is observed in VPRK. Both PRK and VPRKhave ... The ResearchCouncil of Norway and Biotec Pharmacon ASA.References1 Rao MB, Tanksale AM, Ghatge MS & Deshpande VV(1998) Molecular and biotechnological aspects of micro-bial proteases. Microbiol ... within a relatively narrow range(pH 5–8) and are often used in the food industry.Alkaline peptidases are generally highly active aroundpH 10, have broad substrate speciﬁcity and are activeat...

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