Báo cáo khoa học: Molecular characterization of gonad-inhibiting hormone of Penaeus monodon and elucidation of its inhibitory role in vitellogenin expression by RNA interference pptx

... mechanism of RNA control in animals, the function of a diverse number of RNA- binding proteins has been elucidated [1–4]. Despite this, translational control through the binding of RNA- binding proteins ... poorly described in plants. PUF proteins are a large family of RNA- binding proteins found in all eukaryotes. These proteins reduce the expression of mRNA targets...

Ngày tải lên: 18/02/2014, 06:20

... because in terms of func- tion, the fish intestine is highly regionalized both in the larva and in the adult [38]. The posterior segment of the fish intestine is the absorption site of intact pro- teins, ... lining the digestive tract lumen [43,44]. Considering the importance of the intestinal barrier in the process of oral prion infection, this find- ing might help clarify t...

Ngày tải lên: 19/02/2014, 16:20

... allergen- induced histamine release was calculated as percent of the total amount of histamine determined after lysis of the basophils by twofold freezing and thawing of the cells. A histamine release ... participating in an IgE-binding epitope. The N-terminal sequencing of a 52-kDa glyco- protein of tomato extract gave hints for the existence of b-fructofuranosidase as a...

Ngày tải lên: 21/02/2014, 00:20

... cassette, resulting in a Scpep1 ⁄ b-galactosidase ⁄ neo- mycin phosphotransferase fusion protein. The fusion protein is devoid of the C-terminal half of Scpep1, including two amino acids of the assumed catalytic ... 1. Molecular forms of Scpep1. (A) Analysis of molecular forms of Scpep1: 100 lg of cell lysates (C) and 50 lL of medium (M) of HT1080 and HT1080-Scpep1...

Ngày tải lên: 07/03/2014, 03:20

... structures. At 10 min, punctate staining diminished and vacuolar staining in- creased, and finally punctate staining was almost lost with predominant vacuolar staining at 30 min. Com- pared with ... of Medicine, National University of Singapore, Republic of Singapore 2 Institute of Molecular and Cell Biology, A*STAR Biomedical Research Institutes, Singapore, Republic of Sin...

Ngày tải lên: 07/03/2014, 21:20

... the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248–254. 19. Weiss, R. (1970) The solubility of nitrogen, oxygen and neon in seawater. ... Sprinzl, M. (1992) Molecular cloning and nucleotide sequence of the gene encoding a H 2 O 2 -forming NADH oxidase from the extreme thermophilic Thermus thermophilus H...

Ngày tải lên: 08/03/2014, 02:21

... : 26 of the original volume using Centricon-10 (Amicon). Proteins were determined by the modified method of alkaline copper (Lowry) protein assay [23] using BSA as the standard. Transglutaminase ... incorporation of monodansylcadaverine into N,N-dimethylc asein with K m and V max values of 0.35 m M and 13.3 n molÆmin )1 Æmg )1 , respectively, indicating that the predicted protein...

Ngày tải lên: 08/03/2014, 10:20

... cascade involving multiple proteases. In endoparasites, aspartic, cysteine and metalloproteas- es are found in the intestine, and the analysis of Hb proteolysis by those recombinant proteases indicates that ... staining kit (Dai- ichi-Pure Chemical, Tokyo, Japan). The densities of stained bands were measured using image j 1.36b (National Insti- tutes of Health) and the ratio...

Ngày tải lên: 16/03/2014, 10:20

... tivities and s ome properties of 5¢-nucleotidase, adenosine kinase and adenosine deaminase in tissues of vertebrates and invertebrates in relation to the control of the concentration and the physiological ... screen was by n o means exhaustive, and although t he protein kinases tested in this study represent most of the p rincipal classes of protein serine/threonin...

Ngày tải lên: 16/03/2014, 18:20

... unfolding of the proteins is required for dissociation of the complex and membrane binding. In the presence of membrane mimetics, the a-helical contents of Rv3619c and Rv3620 increased by 42% and ... respectively. In mice, the immune response against Rv3619c pro- tein is characterized by increased levels of interferon-c, interleukin-12 and IgG 2a , indicating a domin...

Ngày tải lên: 22/03/2014, 16:21