Báo cáo khoa học: Characterization of Mycobacterium tuberculosis nicotinamidase/pyrazinamidase ppt

... clinical Mycobacterium tuberculosis isolates from Korea. BMC Infect Dis 1,4. 13 Lee KW, Lee JM & Jung KS (2001) Characterization of pncA mutations of pyrazinamide-resistant Mycobac- terium tuberculosis ... Mycobacterium tuberculosis PncA H. Zhang et al. 762 FEBS Journal 275 (2008) 753–762 ª 2008 The Authors Journal compilation ª 2008 FEBS Characterization of Mycobacte...

Ngày tải lên: 23/03/2014, 07:20

... functional characterization to date of a member of a topological subclass of AroQ CMs, which may be involved in the etiology of diseases such as tuberculosis [25] which claim many millions of lives every ... chorismate mutase from M. tuberculosis FEBS Journal 272 (2005) 375–389 ª 2004 FEBS 389 Characterization of the secreted chorismate mutase from the pathogen Mycobacter...

Ngày tải lên: 30/03/2014, 15:20

... 10 6 mL )1 . Next, 0.5 mL of propidium iodide staining solution (10 mg of propidium iodide, 0.1 mL of Triton-X, 3.7 mg of EDTA in 100 mL of NaCl ⁄ P i ) was added to 0.5 mL of cell suspension. The ... were analysed in terms of production ⁄ consumption of TG. In mammalian cells, TG may be deleted to meet the needs of cell metabolism, either as a source of energy or as a res...

Ngày tải lên: 18/02/2014, 13:20

... C-terminal sequence of about 90 amino acids of unknown function. The high degree of identity of the Cg-Clp1 and Cg-Clp2 Glyco_18 domains (84% iden- tity) argues for a conservation of the tertiary ... multiplicity of CLPs in humans and insects [14], we hypothesized that homologues of the previously characterized Cg-Clp1 remain to be found in C. gigas. In this article, we report t...

Ngày tải lên: 19/02/2014, 00:20

... followed by mixture with 6 lL of Lipofectamine Plus reagent at room temperature for 10 min. Four microliters of Lipofectamine Plus reagent were diluted in 100 lL of Opti-MEM I-reduced serum med- ium. ... Department of Legal Medicine and Medical Genetics, Gunma University, Japan 2 Division of Medical Genetics and Biochemistry, University of Fukui, Japan 3 Division of Legal Medici...

Ngày tải lên: 19/02/2014, 06:20

... the mechanism of action of PPI1. The analysis of the pH dependence of PPI1- induced activation of the H + -ATPase showed that stimulation decreases dramatically with the increase of pH above pH ... with PPI1 588 His 6 . The analysis of the effect of PPI1 588 His 6 on the dependence of PM H + -ATPase activity on the concen- tration of MgATP (Fig. 3) showed that stimulation...

Ngày tải lên: 19/02/2014, 07:20

... from Tritirachium album Limber. Characterization of the chromosomal gene and expression of the cDNA in Escherichia coli. Eur J Biochem 179, 185–194. Characterization of a Serratia proteinase K-like ... Willassen 1,2 1 Department of Molecular Biotechnology, Institute of Medical Biology, Faculty of Medicine, University of Tromsø, Norway 2 The Norwegian Structural Biology Centr...

Ngày tải lên: 19/02/2014, 07:20

... of all of these genes remains to be clarified. Expression and purification of Sno1p and Snz1p In light of the similarity in the amino acid sequence of Sno1p to that of the glutaminase subunit of ... absorbance of the supernatant was read at 363 nm. The absorbance of the reduced form of APAD (APADH) was linear over the 2–100 l M range of glutamate with a molar extinction co...

Ngày tải lên: 19/02/2014, 12:20

... differences between the TP isoforms in terms of their mechanisms of signaling [18,19], modes of regulation⁄ desensitiza- tion [20–23] and patterns of expression [24], this repre- sents a question of potentially ... (2002) Characteri- zation of the 5¢ untranslated region of alpha and beta isoforms of the human thromboxane A2 receptor (TP). Differential promoter utilization by t...

Ngày tải lên: 19/02/2014, 16:20

... data. Data analyses Data were analysed by means of sigmaplot 2001 (SPSS Science Software Gmbh, Erkrath, Germany). Data for the characterization of the kinetics of electrogenic BSP uptake fitted the Michaelis–Menten ... process. litre/osmol 01234 nmoles of BSP disappeared 0,0 0,4 0,8 1,2 1,6 Fig. 2. The dependence of valinomycin-induced disappearance of BSP on the osmolarity of...

Ngày tải lên: 20/02/2014, 01:20