Báo cáo khoa học: The first phospholipase inhibitor from the serum of Vipera ammodytes ammodytes docx

... understanding of the patho- physiology of this group of enzymes. In this work we present the characterization of the first PLI from the serum of the most dangerous Euro- pean snake, Vipera ammodytes ammodytes. ... to the 25 kDa subunit of the c-type PLI from the serum of A. b. siniticus [4], to the CNF from Crotalus durissus terrificus and 23 ⁄...

Ngày tải lên: 23/03/2014, 07:20

... alignment of langue maternelle (mother tongue) with 母国•語 bokoku • go (literally [[mother- country]-language]). The usage of thesauri en- abled the system to include the concept of coun- try in the ... comparison of all the methods is illustrated in the precision – valid alignments curves of Figure 2. The points on the four curves are taken from Tables 2 to 5...

Ngày tải lên: 17/03/2014, 22:20

... the product of the weights of all the tokens in the sequence. The aim of this new weighting scheme, as detailed in the next section, is to eliminate the bias caused by the special structure of ... X.a 2 be the two arguments associated with the bag. • Let C(X) be the size of the bag (i.e. the num- ber of sentences in the bag), and C(X, w) the number o...

Ngày tải lên: 23/03/2014, 18:20

... in the cDNA sequence. Numbering of the cDNA sequence begins at the first codon position of the first in-frame methionine. The exact intron size is given where known; estimates were made from the ... found that the 145 kDa isoform disappeared (Fig. 4B). These two observations, the presence of the 70 kDa isoform in gel purified 145 kDa extract, and the disappear...

Ngày tải lên: 30/03/2014, 20:20

... simply changing the sequence of a pre-existing turn. AroQ CMs have composite active sites, consisting of residues from helix 1 of one monomer and residues from helices 2 and 3 of the other monomer. ... CMs catalyze the Claisen rearrangement of chorismate to prephenate, which is the first committed step in the biosynthesis of phenylalanine and tyrosine [11]. In thi...

Ngày tải lên: 19/02/2014, 12:20

... possibilities for the design of selective inhibitors, although their presence in the second shell of the catalytic site, rather than in the first (Fig. 4) raises doubts as to their accessibility ... shared with another pathogenic trypano- somatid, L. major. The presence of these unique residues offers interesting opportunities for the design of inhibitors selective for...

Ngày tải lên: 23/03/2014, 21:20

... decreasing the gap to 5.7%. Now, let us turn to the question of the utility of the constraints. First, observe that the non-regularized version of the model (NoReg) often fails to outper- form the baseline ... differences in the marginal distributions between the domains. In fact, we do not need to consider the behavior of the classifier to understand the ration...

Ngày tải lên: 30/03/2014, 21:20

... [6] and inhibits the tyrosine kinase activity of the IR [7]. On the basis of the crystal structure of the tyrosine kinase domain of the IR in complex with the PIR ⁄ BPS domain of Grb14, Depetris ... we cannot assess whether the kinase activity of the IRs before KCl treatment repre- sents residual activity of receptors with Grb14 bound to them, the activity o...

Ngày tải lên: 18/02/2014, 18:20

... refolding of the recombinant protein, we subjected the protein to amino acid sequencing. At the N-terminus of the recombinant UcPLA 2 , we discovered a methionine pre- ceding the first amino acid of the ... enzyme. In the presence of EGTA, it showed no enzymatic activity on either of the substrates (Fig. 5). In the presence of NaCl, its enzymatic activity foll...

Ngày tải lên: 06/03/2014, 11:20

... the absence of cinnamaldehyde the growth of the strains A B Fig. 4. Analysis of the pH optimum and temperature stability of YagTSR. (A) The pH optimum of YagTSR was determined by analysis of the specific ... an activity of 27.9 ± 0.2 UÆmg )1 (Table 1). Cofactor analysis of the purified YagTSR proteins To analyze the cofactor content of YagTSR expressed in the...

Ngày tải lên: 23/03/2014, 04:21