Báo cáo khoa học: Something from nothing ) bridging the gap between constraint-based and kinetic modelling pot

... 1 ETOH ) 2.95 ) 1 NAD ) 3.04 ) 1 NADH 3.20 1 ALD F16P 1.89 1 TRIO ) 3.08 ) 2 ATP P 1.80 1 ENO P2G 0.826 1 PEP ) 0.384 ) 1 GAPDH BPG ) 8.00 · 10 )2 ) 1 NAD 0.144 1 NADH ) 9.14 · 10 )2 ) 1 TRIO ... Michaelis–Menten kinetics v(x) ¼ Vx⁄ (x + K m ) (o) to its linlog approximation u(x) ¼ v*(1+e log(x ⁄ x *)) (solid line) and its power l...

Ngày tải lên: 23/03/2014, 07:20

... splicing (Fig. 6B) and therefore it seems reasonable to make the working hypothesis that the RNA and DNA binding sites are not the same, rather that they are separate. This does not exclude the possibility ... to result from slow release of the cleavage products from the protein. The amplitudes of the initial burst (2.78 and 2.83 n M ) gave a ratio of DNA/protein...

Ngày tải lên: 17/03/2014, 10:20

... plasma) were then added to wells coated overnight with LM (10 lgÆmL )1 ), VN (5 lgÆmL )1 ) and FN (10 lgÆmL )1 ), respectively, and blocked with Locke’s buffer contain- ing 1% (w ⁄ v) BSA. (B) Alternatively, ... Purified HK and HKa Ligand coated on the microtiter plate Fig. 1. Extraction of HK from plasma (A) and of HK and HKa (B) from solutions of purified...

Ngày tải lên: 30/03/2014, 01:20

... Oligonucleotides were synthesized by Eurogentec (Liege, Belgium). The pET23a( +) expression vector and E. coli strain BL21(DE 3) were from Novagen (San Diego, CA, USA). All other chemicals were from commercial ... was measured in the presence of the mediator 2,6-dichlorophenolindophenol at 600 nm, the activity with potassium ferricyanide was measured at 420 nm (e 420 =1mm...

Ngày tải lên: 23/03/2014, 07:20

... d j ). N T (d j ) D T (d i ) ( 3) where N T (d j ) is the number of times the walk has visited d j up to time T and D T (d i ) =  d j ∈V p 0 (d i , d j )N T (d j ) ( 4) Here, p ∗ (d j ) is the ... (Erkan and Radev, 200 4)) . Once the network is built, the system finds the most central sentences by performing a random walk on the graph. p(d...

Ngày tải lên: 30/03/2014, 21:20

... Mcl-1 and A 1), pro-apoptotic proteins (Bax, Bak and Bok) and BH3-only proteins (Bad, Bid, Bik, Blk, Hrk, BNIP3 and BimL). Bcl-2 family proteins mostly mediate the activity of other proteins in the ... phosphorylation of the MAPK family and mitochondrial proteins. Phosphorylated ERK prevents the activation of caspases and the Bcl-2 family, whereas these are activated b...

Ngày tải lên: 14/02/2014, 22:20

... manufacturer. The reactions contained 5 lL of SYBR Green PCR Core Kit (Bioke, Leiden, The Netherlands), 3 pmol of each primer (Isogen, De Meern, The Netherlands or Biolegio, Nijmegen, The Netherlands) and ... understand the patterns of enzyme capacities over time, we should rather consider them as the result of a balance between protein synthe- sis and breakdown from the...

Ngày tải lên: 18/02/2014, 06:20

... one (or more) of them in the segment between positions 402 and 408. In the complementary experiment, the C-terminal fragment of Rio1p (335–484p) was heavily phosphorylated (Fig. 3C). These results ... a (encoded by CKA 1) and a¢ (encoded by CKA 2), and two regulatory b subunits, b and b¢ in Saccharomyces cerevisiae (CKB1 and CKB 2). The enzyme occurs in all possible...

Ngày tải lên: 18/02/2014, 16:20

... activation actions: {l) whether the speaker intends that the hearer identify the denotation of the de- scription, and { 2) how much mutual knowledge the speaker and hearer share about the description's ... axiomatize the relationship that holds between the terms and the individuals they denote. The terms can consist of predicates combined with an iota o...

Ngày tải lên: 21/02/2014, 20:20

... Comparison of the stability of ChOxs, from different sources, in the absence (A) and in the presence of 0.1% (B) and 0.5% (C) Triton X-100 (black bars) or sodium cholate (white bars). The enzyme ... (a) total and esterified serum; (b) from low-density lipoproteins to high-density lipo- proteins; (c) on the cell membrane of erythrocytes (and of other cells and cellular co...

Ngày tải lên: 07/03/2014, 00:20