Báo cáo khoa học: Probing the active site of Corynebacterium callunae starch phosphorylase through the characterization of wild-type and His334fiGly mutant enzymes pot

... that of the wild-type and with an optimum pH of 6.5. A shift of the pH profile of about + 0.5–1.0 pH units and an optimum pH similar to that of the wild-type was observed for the H334G mutant in the ... Characterization of PLP phosphate in wild-type CcStP and the H334G mutant using 31 P-NMR. (A) Spectra of wild-type CcStP and the H334G mutant...

Ngày tải lên: 23/03/2014, 07:20

... F 0 , F c and DF max represent the fluorescence intensity of tubulin in the absence of rotenone, the fluorescence intensity of tubulin in the presence of different concentrations of ligand and the maximum ... a variety of cell types and several mechanisms, such as activation of the Jun N-terminal kinase pathway, involvement of the caspase activated DNAase, t...

Ngày tải lên: 07/03/2014, 05:20

... special function of E393 can be proposed on the basis of the results presented here. Two of the mutants (D217E and E221Q) showed no activity in any of the assays. These mutants (and wild- type) ... reactions at a single active site [1]. One of the main purposes of this study was to try to dissect the two reactions by mutagenesis of active site residues, ex...

Ngày tải lên: 16/03/2014, 18:20

... determined at pH 5 and 0.05% SDS, and DO activity at pH 7 and 0.02% SDS. Fig. 3. Dependence of the TH activity of extracts of R3 and the two mutant strains on the presence of cofactor, L-dopa. ... but the differentiation between these two types of enzyme is not clear [4]. Looking at the sequences of the two enzymes, both show absolute conservation of the...

Ngày tải lên: 19/02/2014, 07:20

... trunca- tions, the availability of the Tyr190 mutants makes it feasible to conduct cell-based studies of the effects of the stability of the acetylated enzyme intermediate on the N-acetylation of aromatic ... hypothesized that modulation of the cata- lytic potential of the catalytic triad might be accessible through point site mutations of the proximal re...

Ngày tải lên: 16/03/2014, 00:20

... L35S/F46Y, and K31A/ R33S/F46Y) and studied the effect of the mutations on the thermodynamic and kinetic stability. The similarity of the impact on both the thermodynamic and kinetic stabilities suggests ... stabilized by a network of hydrogen bonds between the side chain of Arg33 and the backbone of Met13 and Arg10 (three hydrogen bonds) and between t...

Ngày tải lên: 16/03/2014, 18:20

... reduction of the Cys residue at position 430 of AT, it is unlikely that the epitope is at the very C-terminus of the peptide. The results therefore suggested that residues 411 and 416 are part of the ... to form the critical hydrogen bond of the catalytic triad – a bond that is a prerequisite for cleavage of the acyl intermediate that links the protease to t...

Ngày tải lên: 17/03/2014, 10:20

... requires further structural insight is, on the one hand, the widespread use of Asp at the P7 position in many naturally occurring NADPH-dependent GDHs and, on the other hand, the failure of the E243D ... attained for the wild-type enzyme, nor any of the mutants. Accordingly, the measurements in the presence of oxo- glutarate were the most useful for overall co...

Ngày tải lên: 30/03/2014, 03:20

... position of the substrate was determined on the basis of the possible binding sites identified by the Site Finder function in moe, as well as the relative position of the indole substrate in the crystal ... nmolÆmin )1 Æmg )1 protein. On the other hand, the 24DMA activity of the V205A mutant was reduced to 0.1 nmolÆmin )1 Æmg )1 protein (Table 2). The 24DMA co...

Ngày tải lên: 19/02/2014, 02:20

... to judge the information content of the translations, the translation quality, and the user-friendliness. The list of criteria for self evaluation consisted of technical, linguistic and ergonomic ... used for comparing the sizes of lexicons since the number of error tokens is given rather than the number of error types. Furthermore it is questionable if a r...

Ngày tải lên: 22/02/2014, 03:20