... (196 9) Inhibitorsaround the antimycin-sensitive site in the respiratorychain. In Inhibitors ) Tools in Cell Research (Bucher Th& Sies H, eds), pp. 282–300. Springer, Berlin.39 Gonzalez ... NADHgeneration, due to amino acid biosynthesis. Much of the generation of NADH during amino acid biosyn-thesis takes place in the mitochondria. Because of the block in the respiratory chain caused by the additionof ... 25 lm). In thiscase, uracil (50 mgÆL )1 ) was also added, because in someyeasts, de novo pyrimidine biosynthesis is coupled to the respiratory chain [8]. When specified in the text, lysine andglutamic...