Báo cáo khoa học: Structural properties of semenogelin I docx

Báo cáo khóa học: Structural properties of the protein SV-IV potx

... Italy;5Dipartimento di Scienza degli Alimenti, Universita`degli Studidi Napoli ‘Federico II’, Italy;6Istituto Internazionale di Genetica e Bioﬁsica, CNR, Napoli, ItalyWe have investigated the ... University Napoli, Italy;3Centro di Ricerca Interdipartimentale di Scienze Computazionali e Biotecnologiche, Napoli, Italy;4Istituto di Scienze dell’Alimentazione, CNR, Roma, Italy;5Dipartimento ... energy minimization,Fig. 3. Protein digestion. Chromatograms of digestion mixtures of trimeric (A) and monomeric (B) forms of SV-IV after 12 h incubation withtrypsin. Each peak is labelled with...

Tài liệu Báo cáo khoa học: Solution properties of full-length integrin aIIbb3 reﬁned models suggest environment-dependent induction of alternative bent ⁄extended resting states doc

... rota-tion of the lower b3leg already accompanies extension,bringing the activating residues into contact with otherresidues in the aIIbsubunit. In addition, some cautionshould be exerted in ... Mattia Rocco21 Nanobiotecnologie, Istituto Nazionale per la Ricerca sul Cancro (IST), Genova, Italy2 Biopolimeri e Proteomica, IST, Genova, ItalyIntroductionIntegrins are heterodimeric transmembrane ... (1994)CLUSTAL W: improving the sensitivity of progressivemultiple sequence alignment through sequence weight-ing, position-speciﬁc gap penalties and weight matrixchoice. Nucleic Acids Res 22 ,...

Tài liệu Báo cáo khoa học: Structural features of proinsulin C-peptide oligomeric and amyloid states pptx

... C-pep-tide is involved in the disaggregation of insulin, increas-ing insulin bioavailability by monomerization [12,13].C-peptide itself has been shown to adopt unorderedstructures in aqueous ... presenceFig. 1. Oligomer formation of proinsulinC-peptide is affected by metals and insulin.Prosinsulin C-peptide oligomer formation asa function of time (A). C-peptide was incu-bated for the indicated ... zinc-inducedhexamers in vivo with deferred bioactivity. Other stud-ies revealed that insulin also can form amyloid-likestructures in vitro [22], with proinsulin being less sus-ceptible to ﬁbrillation than insulin...

Tài liệu Báo cáo khoa học: Structural effects of a dimer interface mutation on catalytic activity of triosephosphate isomerase The role of conserved residues and complementary mutations pptx

... substi-tutions at these sites do not signiﬁcantly impair enzymeactivity [28]. Interestingly, the bulky Trp residue isfound at this position in the sequence of TIM fromGiardia lamblia (GlTIM) ... labile.DiscussionEffects of the Y74W mutationResidue 74, which lies at the dimer interface of PfTIM,appears to be important in promoting subunit dissocia-tion [27] and also in maintaining ... full-length ampliﬁed product (746 bp)containing the desired mutation was puriﬁed using a genecleaning kit (Qiagen, Qiagen India, Genetix Biotech Asia,New Delhi, India), digested with enzymes NcoI and...

Tài liệu Báo cáo khoa học: Electrical properties of plasma membrane modulate subcellular distribution of K-Ras ppt

... electrostatic interaction, we analyzedin vitro the reversibility of such interaction. CytosolicA i iiiiiBCFig. 3. Membrane binding properties of K-Ras. (A) Membrane fractions of CHO-K1 cells ... membranes. Inhibition of synthesis of polyphosphoinositides (poly PIs) in livecells, by depletion of cellular ATP, resulted in signiﬁ-cant accumulation of K-Ras in a perinuclear region,G. A. ... membranelocalization of K-Ras, and are likely to be of consider-able relevance in K-Ras signaling in physiological andpathological cell conditions.The role of cytoplasmic composition of anionic li-pids in...

Tài liệu Báo cáo khoa học: Structural characterization of Ca2+/CaM in complex with the phosphorylase kinase PhK5 peptide pdf

... Castiglione Morelli MA, Strazzullo G,Fucile P, Gautel M & Motta A (2001) Kinase recogni-tion by calmodulin: modeling the interaction with theautoinhibitory region of human cardiac titin kinase.J ... (1991) Triple-resonance multidimensional NMR study of calmodulincomplexed with the binding domain of skeletal musclemyosin light-chain kinase: indication of a conforma-tional change in the ... troponin C [3].PhK is related to other Ca2+⁄ CaM-dependent proteinkinases including myosin light chain kinase (MLCK),CaM kinases I, II and IV (CaMKI, CaMKII andCaMKIV, respectively), CaM kinase...

Tài liệu Báo cáo khoa học: Inhibitory properties of cystatin F and its localization in U937 promonocyte cells ppt

... modulatoryinhibitors are often colocalized with their targets. Cyst-atin F could be a candidate for modulating the activity of cathepsin H; in vitro inhibition is tight enough toimpair its activity ... prolonged incubation at dithiotrei-tol concentrations as high as 100 mm, and the K i valuefor the inhibition of papain (K i ¼ 1.4 nm) was similarto that reported for inhibition at low dithiotreitol ... of cystatin F is linked to disulﬁde bond formation, which isresponsible for the loss of inhibitory activity of the pro-tein. We also noticed that monomerization of cystatin Fwas enhanced in acidic...

Tài liệu Báo cáo khoa học: "Structural Definition of Affixes from Multisyllable Words" docx

... sequence tic is frequently encountered followed by al, ize, or ide to form tical, ticism, ticize, or ticide, as in elliptical, asepticism, didacticism, ascepticize, romanti- cize, and infanticide. ... utilitarian evaluation of their validity can be made after the syntactic and phonetic implica- tions of the defined affixes have been investigated. The definitions for affixes given in this ... V3C3V2C2V1C1, if either C2 or C3 is an admissible final string but not an admissible initial string, everything following that consonant string is a suffix possibility. The criterion by which an...

Tài liệu Báo cáo khoa học: Enzymatic properties of wild-type and active site mutants of chitinase A from Vibrio carchariae, as revealed by HPLC-MS pptx

... Rapid and sensitive method forthe quantitation of microgram quantities of protein util-izing the principle of protein-dye binding. Anal Biochem72, 248–254.33 Laemmli UK (1970) Cleavage of structural ... of a chitinasefrom the pathogenic fungus Coccidioides immitis.Protein Sci 29, 544–551.29 Watanabe T, Kobori K, Miyashita K, Fujii T, Sakai H,Uchida M & Tanaka H (1993) Identiﬁcation of ... &Fothergill-Gilmore LA (2000) Chitinases from Vibrio:activity screening and puriﬁcation of Chi A from Vibriocarchariae. J Appl Microbiol 89, 76–84.25 Suginta W, Vongsuwan A, Songsiriritthigul...

Tài liệu Báo cáo khoa học: Transactivation properties of c-Myb are critically dependent on two SUMO-1 acceptor sites that are conjugated in a PIASy enhanced manner pptx

... Italy;3Dipartimento di Biochimica, Bioﬁsica e Chimica delle Macromolecole, Universita`degli Studi di Trieste, ItalyThe transcription factor v-Myb is a potent inducer of myeloid leukemias, and its cellular ... Norway.Fax:+4722854443,Tel.:+4722857346,E-mail: o.s.gabrielsen@biokjemi.uio.noAbbreviations: DBD, DNA-binding domain; MRE, Myb recognitionelement; NRD, negative regulatory domain; PIAS, protein inhibitior of activated STATs; ... conjugation, it is rather unlikely that a singleexplanation for the biological role of sumoylation will befound. A more general proposition is that sumoylation playsa role in the stabilization of...

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