Báo cáo khoa học: A transcription factor of lipid synthesis, sterol regulatory element-binding protein (SREBP)-1a causes G1 cell-cycle arrest after accumulation of cyclin-dependent kinase (cdk) inhibitors pdf

... primer, 5¢-ACACTGGCGGCCTGACAA AG-3¢,5¢ primer, 5¢-AGCTCGTAGTAAGGGCCAATGA ATGTTCT-3¢; p27: 3¢ primer, 5¢-CAAAACCGAACAAA AGCGAAACGCCA-3¢,5¢ primer, 5¢-CAACCCATCCAA ATCCAGACAAAAT-3¢. All constructs ... Shimano H, Nakakuki M, Matsuzaka T, Nakagawa Y, Yamamoto T, Sato R, Takahashi A, Sone H, Yahagi N et al. (2005) Lipid synthetic tran- scription factor SREBP- 1a activates p21WAF1 ⁄ CIP1, a...

Ngày tải lên: 23/03/2014, 07:20

... reverse, 5¢-AGGAGCCTTTGGTGAGTTCAATT- AT-3¢; OGG1 forward, 5¢-CGCTATGTATGTGCCA GTGCTAAA-3¢; OGG1 reverse, 5¢-CCTTAGTCTGCGAT GTCTTAGGCT-3v; GAPDH forward, 5¢-GACAACTTTG GTATCGTGGAAGG-3¢; and GAPDH reverse, ... lg of total RNA, with a ReverTra Ace reverse trans- criptase kit (Toyobo Co. Ltd. Osaka, Japan). The RNA and cDNA of each sample were assayed with the gene quant pro dna ⁄ rna calcu...

Ngày tải lên: 14/03/2014, 23:20

... 5¢-AGTTTCGCTCTTGTCT CCCAG-3¢; P1 antisense, 5¢-ATGGCGAAACCCTGTCTC TAC-3¢; P2 sense, 5¢-AGACAGCCGTTTTACACGCAG-3¢; P2 antisense, 5¢-CACCGAGAAATCGAAATCACC-3¢;P3 sense, 5 ¢-TAGGAAGGTTGTATCGCGGAGG-3¢; and P3 antisense, ... Quantitative estimation of p16 mRNA level. Cells were transfected with HDAC3 or HDAC4 vector. Total RNA was isolated and reverse transcribed, and p16 mRNA was measured by real...

Ngày tải lên: 07/03/2014, 02:20

... Haematolog- ica 89, 1332–1340. 42 Naito K & Fujikawa K (1991) Activation of human blood coagulation factor XI independent of factor XII. Factor XI is activated by thrombin and factor XIa ... FXI-deficient plasma as sub- strate (Hemoliance, Salt Lake City, UT). FXI antigen was measured by an ELISA based on a goat anti-human FXI affinity purified IgG as capture antibody and a g...

Ngày tải lên: 23/03/2014, 07:20

... for amplification of the NOX4 promoter were as follows: 5¢-AACAATCAGTCTAAAAGAGCTGTGTCTT CT-3¢ (forward for GC-box1), 5¢-CTCCAAAATACTGG CAAACATGTGAACAAT-3¢ (reverse for GC-box1), 5¢- TGAGTGGGCAGAGCTGACCCGGTGCGGGT-3¢ ... Sp3 transcription factor is crucial for transcriptional activation of the human NOX4 gene Masato Katsuyama 1,2 , Hideyo Hirai 3 , Kazumi Iwata 2 , Masakazu Ibi 2 , Kunih...

Ngày tải lên: 29/03/2014, 00:20

... contain a carbohydrate chain; ApoC3-1, ApoC3 with a GalNAc-Gal-sialic acid carbohydrate chain; ApoC3-2, ApoC3 containing the carbohydrate of ApoC3-1 plus an additional sialic acid residue; DPPase, ... background of at least 1000 subjects was typical of the American Midwest with  90% of European ancestry and  5% each of African and Asian ancestry. An exception was the targeted...

Ngày tải lên: 19/02/2014, 05:20

... & Brade, H. (1994) Preparation and structural analysis of oligosaccharide monophosphates obtained from the lipopolysaccharide of recombinant strains of Salmonella minnesota and Escherichia coli ... Gram-negative bacteria, and their adaptability to many different pollutants [1]. Pseudomonas stutzeri OX1 is a Gram-negative bacterium isolated from the activated sludge of a wastew...

Ngày tải lên: 19/02/2014, 13:20

... mRNA accumulation in transgenic Arabi- dopsis thaliana. Plant Cell Physiol. 42, 1174–1180. 46. Hacham, Y., Avraham, T. & Amir, R. (2002) The N-terminal region of Arabidopsis cystathionine gamma-synthase ... can be calculated as follows: ELISA assays were carried out using rabbit antibodies raised against the recombinant proteins [12,14] and purified proteins as standards. We measured t...

Ngày tải lên: 20/02/2014, 02:21

... (A) Fractionation PCR. A partial fragment of the mitochond- rial large subunit ribosomal RNA (23S rRNA) was amplified by PCR, using fraction samples that contained equal amounts of protein. Lane, ... kb. To date, there has been no evidence of an association between mitochondria and Tetrahym- ena cell death, and mitochondrial homologues of mam- malian apoptosis factors, such as AIF,...

Ngày tải lên: 20/02/2014, 03:20

... src="data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAABKYAAAYwCAIAAADH3NDGAAAACXBIWXMAABYlAAAWJQFJUiTwAAAgAElEQVR42uzdf2zV9b348c85LeXQlhbhSCc17YFVqAJBma3bWsicyiVwvXpFut3e 9A/ ZLvOi3Ft3k1VJ0+QyIvQumd1gxmsydnOr 9a4 4wItwGZfNrdS7rOXC7YQruav2xyRbba1QaltKe873jyZk2Xd3t8fLpYKPx1+mhJfH97sJPvm8+/6EEolEAAAAwPUobAkAAAAkHwAAAJIPAAAAyQcAAIDkAwAAQPIBAAAg+QAAACQfAAAAkg8AAADJBwAAgOQDAABA8gEAACD5AAAAJB8AAACSDwAA...

Ngày tải lên: 20/02/2014, 04:20