... and a mature protein starting with Ile59, in accord with the result of N-terminal protein sequence analysis. It is likely that the mature protein is composed of 496 amino acids with a calculated molecular ... 2006) doi:10.1111/j.1742-4658.2006.05570.x Fish skin mucus contains a variety of antimicrobial proteins and peptides that seem to play a role in self defense. We pr...
Ngày tải lên: 30/03/2014, 10:20
Báo cáo khoa học: Identification of an osteopontin-like protein in fish associated with mineral formation pot
... Osteopontin and osteopontin-like gene and protein struc- ture. (A) Structural organization of osteopontin-like (tetraodon and zebrafish) and osteopontin (chicken, bovine, mouse and human) coding sequences ... OP-L protein is indeed the fish equivalent of mammalian osteopontin. OP-L protein plays a role in the process of mineralization Osteopontin is a multifaceted protein...
Ngày tải lên: 23/03/2014, 07:20
... Aeschlimann P, Langley M, Blain E, Errington R, Kipling D & Aeschlimann D (2004) Cross-linking and G -protein functions of trans- glutaminase 2 contribute differentially to fibroblast wound healing ... by CBB staining (right) and immunoblotting analysis using each polyclonal antibody (left): (A) anti-CBP40; (B) anti-Physarum actin; (C) anti-PpANT. The closed arrows indicate each protei...
Ngày tải lên: 23/03/2014, 09:20
Báo cáo khoa học: Identification of an atypical insect olfactory receptor subtype highly conserved within noctuids potx
... E, Bohbot J, Francois MC, Cain AH & Nagnan-Le Meillour P (2000) Characterization of the general odorant-binding protein 2 in the molecular coding of odorants in Mamestra brassicae . Eur J Biochem ... found in beneficial insects such as the honeybee [17], thus precluding the use of molecules interfering with OR83b receptors. Another subfamily of insect ORs, restricted to m...
Ngày tải lên: 29/03/2014, 22:21
Báo cáo khoa học: Identification of tyrosine-phosphorylation sites in the nuclear membrane protein emerin pot
... membrane proteins of the INM. The INM proteins form complexes, transiently or stably, with lamins, chromatin proteins and a vari- ety of regulatory proteins, including transcriptional regulators and ... with the actin-binding spectrin-repeat proteins nesprin 1a and nesprin 2 [28,29]. Since the different binding regions overlap at least partially, a simultaneous binding of some bindi...
Ngày tải lên: 07/03/2014, 12:20
Báo cáo khoa học: Identification of mitogen-activated protein⁄extracellular signal-responsive kinase kinase 2 as a novel partner of the scaffolding protein human homolog of disc-large docx
... of interacting partners and the coordination of their activities. MAGUK contain a number of protein protein interaction domains, such as PSD-95, disc-large, ZO-1 (PDZ), Src-homology 3 and guanylate ... were identified as containing the C-terminal region of the human MEK2 cDNA. To analyze in more detail the interacting sites within hDlg and MEK2, mutant deriv- atives of PGKBT7-PDZ...
Ngày tải lên: 22/03/2014, 16:20
Báo cáo khoa học: Identification of versican as an isolectin B4-binding glycoprotein from mammalian spinal cord tissue pptx
... lane 5, 5 lg of nonprecipitated proteins; lane 6, 5 lg of protein from washing step 1; lane 7, 5 lg of protein from washing step 2; lane 8, half volume of the eluted proteins; lane 9, 30 lg of ... arrow indicates IB4-binding activity): lane 1, native light membranes; lane 2, light membranes after a 2 min incubation with proteinase K; lane 3, light membranes after a 5 min incub...
Ngày tải lên: 07/03/2014, 16:20
Báo cáo khoa học: Identification of sodium salicylate as an hsp inducer using a simple screening system for stress response modulators in mammalian cells pptx
... blot of Luc protein in the supernatant of cells treated with or without 30, 45 and 60 m M SA at 37 °C. Table 1. Effect of geldanamycin, curcumin, or geranylgeranylacetone on Hsp105 promoter in pGL105/C3H ... showed that SA induced the activation of HSF, the transcription of hsp genes and the accumu- lation of Hsps in various mammalian cells and a concomi- tant increase o...
Ngày tải lên: 17/03/2014, 10:20
Tài liệu Báo cáo khoa học: Identification of Ewing’s sarcoma protein as a G-quadruplex DNA- and RNA-binding protein ppt
... DNA-binding motif in nucleolin [21– 27]. The RGG domain of FMRP, which is an RNA- binding protein involved in nerve cell differentiation, interacts with the G-quartet forming RNA [22–25]. In addition, ... 4 and 6) was incubated with (lanes 1, 2, 5 and 6) or without (lanes 3 and 4) PRMT3 in a potassium buffer with (lanes 3–6) or without (lanes 1 and 2) AdoMet. (B) Schematic...
Ngày tải lên: 15/02/2014, 01:20
Tài liệu Báo cáo khoa học: Identification of two late acyltransferase genes responsible for lipid A biosynthesis in Moraxella catarrhalis doc
... Zeocin resistance cassette Invitrogen pSlpxX-zeo Zeocin resistance gene inserted into pSlpxX This study pUC4K Kanamycin resistance cassette Amersham pSlpxL-kan Kanamycin resistance gene inserted ... pWW115, Shengqing Yu for advice on the pulmonary clearance assay, R. Morell for help with DNA sequencing, Yandan Yang for help with Southern blotting, and Lina Zhu and Yili Chen for help in...
Ngày tải lên: 18/02/2014, 14:20