Báo cáo khoa học: GCN4 enhances the stability of the pore domain of potassium channel KcsA potx

... GCN4 enhances the stability of the pore domain of potassium channel KcsA Zhiguang Yuchi, Victor P. T. Pau and Daniel S. C. Yang Department of Biochemistry and Biomedical Sciences, Faculty of ... the wild-type, the C-terminal domain deleted KcsA cannot form tetramers in vivo. Nevertheless, whether the lack of expression of the tetramer is caused by...

Ngày tải lên: 23/03/2014, 06:20

... substrate to one of the two domains can also stabilize the structure of the other domain [32–34]. Often the results of studies with the solubilized PGK do not support the suggestion from the X-ray crystallographic ... PGK that lacks the ability of domain closure. Structural origin of the increased stability of the closed conformation In the structures...

Ngày tải lên: 16/03/2014, 18:20

... close to the N-terminus of the frag- ment ) it lies between the start of the fragment and the predicted start of the domain (Fig. 1). From the round 3 mutants, three were selected for further char- acterization: ... ZnCl 2 inhibits the activity of AMPP [1]. Here, the presence of ZnCl 2 in the dialysis buffer led to the precipitation of each of the three...

Ngày tải lên: 23/03/2014, 07:20

... to other proteins of this class, such as the DNA-binding domain of the Mu repressor [21]. Third, the backbone atoms of the region comprising the ÔwingÕ b-sheet (residues 2–4, 35–36 and 41–44 of ... or another Xis molecule. The amino acid substitution of Cys28 fi Ser did not significantly change either the structure or stability of HK022 Xis. The differences obser...

Ngày tải lên: 23/03/2014, 15:21

... significantly increased stability with a half-life of  8 h (Fig. 3B and quantified in Fig. 3C), confirming the importance of this domain in the regulation of TSC2 stability. To investigate further the mechanism ... role of the death domain in the control of DAPK stability. In summary, we have shown that TSC2 can promote the lysosome-dependent degradation of DAPK...

Ngày tải lên: 28/03/2014, 23:20

... = 5. Find the fourteenth digit after of the decimal point of We have : the digit 5 can have rounded We try the digit 5 can not have rounded We set : The second power of two sides of equation ... 1 The number contains the end two digits are digit 1 The number contains the end three digits are digit 1 The number contains the end four digits are digit 1 Answer...

Ngày tải lên: 21/10/2013, 16:11

... of all mutations in the BTK gene are missense and some of these reduce the stability of the protein. This is exemplified by mutations in the BTK motif of the Tec homology (TH) domain [18]. This ... Conse- quently, the PH–TH domain doublet of ITK fuses directly with the linker B kinase region of SYK. The PH domain of TFKs usually binds to phosphatidylino- s...

Ngày tải lên: 14/02/2014, 18:20

... (unit: C 6 h )1 Æg )1 FW): r CO 2 !Suc ¼ r NetPhotosynthesis Àr CO 2 !Starch Ratesof net photosynthesis (r NetPhotosynthesis ) were calcu- lated as the average rate of carbon uptake during the first half of the light phase (n = ... interconversion rates as a function of time of cold exposure, (b) comparison of the magnitudes of the various interconversion rates, and (c) l...

Ngày tải lên: 14/02/2014, 22:20

... and the decreased leptin levels are absent in female mice, suggesting a gender specificity of the bone response. Further assessment of the role of NPY in the control of bone homeostasis – the ... hypothalamus, the hypothesis that leptin controls bone formation via a central mechanism was raised. The most convincing evidence supporting this hypothesis was the rescue of...

Ngày tải lên: 18/02/2014, 04:20

... conformation, the SH2 domain binds to pY i on the C-terminal tail, and the Src homology 3 (SH3) domain binds to the linker between the SH2 and kinase domains at the back of the small lobe, preventing the ... presenting the pulse of Src activity in the plane of the inactive and active fractions, s i and s 1 . If the duration of the stimulus exceeds the c...

Ngày tải lên: 18/02/2014, 11:20