Báo cáo khoa học: GCN4 enhances the stability of the pore do

Báo cáo khoa học: GCN4 enhances the stability of the pore domain of potassium channel KcsA potx

... GCN4 enhances the stability of the pore domain of potassium channel KcsA Zhiguang Yuchi, Victor P. T. Pau and Daniel S. C. Yang Department of Biochemistry and Biomedical Sciences, Faculty of ... the wild-type, the C-terminal domain deleted KcsA cannot form tetramers in vivo. Nevertheless, whether the lack of expression of the tetramer is caused by...

Ngày tải lên : 23/03/2014, 06:20

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Báo cáo khoa học: Correlation between conformational stability of the ternary enzyme–substrate complex and domain closure of 3-phosphoglycerate kinase potx

... substrate to one of the two domains can also stabilize the structure of the other domain [32–34]. Often the results of studies with the solubilized PGK do not support the suggestion from the X-ray crystallographic ... PGK that lacks the ability of domain closure. Structural origin of the increased stability of the closed conformation In the structures...

Ngày tải lên : 16/03/2014, 18:20

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Báo cáo khoa học: Using directed evolution to improve the solubility of the C-terminal domain of Escherichia coli aminopeptidase P Implications for metal binding and protein stability pptx

... close to the N-terminus of the fragment ) it lies between the start of the fragment and the predicted start of the domain (Fig. 1). From the round 3 mutants, three were selected for further char- acterization: ... ZnCl 2 inhibits the activity of AMPP [1]. Here, the presence of ZnCl 2 in the dialysis buffer led to the precipitation of each of the three...

Ngày tải lên : 23/03/2014, 07:20

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Báo cáo khoa học: Solution structure and stability of the full-length excisionase from bacteriophage HK022 pot

... to other proteins of this class, such as the DNA-binding domain of the Mu repressor [21]. Third, the backbone atoms of the region comprising the ÔwingÕ b-sheet (residues 2–4, 35–36 and 41–44 of ... or another Xis molecule. The amino acid substitution of Cys28 ﬁ Ser did not signiﬁcantly change either the structure or stability of HK022 Xis. The differences obser...

Ngày tải lên : 23/03/2014, 15:21

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Báo cáo khoa học: Tuberous sclerosis-2 (TSC2) regulates the stability of death-associated protein kinase-1 (DAPK) through a lysosome-dependent degradation pathway doc

... significantly increased stability with a half-life of  8 h (Fig. 3B and quantified in Fig. 3C), confirming the importance of this domain in the regulation of TSC2 stability. To investigate further the mechanism ... role of the death domain in the control of DAPK stability. In summary, we have shown that TSC2 can promote the lysosome-dependent degradation of DAPK...

Ngày tải lên : 28/03/2014, 23:20

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BÀI BÁO CÁO KHOA HỌC TẠI BẮC KINH ATCM 2009 THE SCIENTIFIC REPORT IN BEIJING - CHINA

... = 5. Find the fourteenth digit after of the decimal point of We have : the digit 5 can have rounded We try the digit 5 can not have rounded We set : The second power of two sides of equation ... 1 The number contains the end two digits are digit 1 The number contains the end three digits are digit 1 The number contains the end four digits are digit 1 Answer...

Ngày tải lên : 21/10/2013, 16:11

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Tài liệu Báo cáo khoa học: TEC family kinases in health and disease – loss-of-function of BTK and ITK and the gain-of-function fusions ITK–SYK and BTK–SYK pptx

... of all mutations in the BTK gene are missense and some of these reduce the stability of the protein. This is exempliﬁed by mutations in the BTK motif of the Tec homology (TH) domain [18]. This ... Conse- quently, the PH–TH domain doublet of ITK fuses directly with the linker B kinase region of SYK. The PH domain of TFKs usually binds to phosphatidylino- s...

Ngày tải lên : 14/02/2014, 18:20

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Tài liệu Báo cáo khoa học: A systems biology approach for the analysis of carbohydrate dynamics during acclimation to low temperature in Arabidopsis thaliana doc

... (unit: C 6 h )1 Æg )1 FW): r CO 2 !Suc ¼ r NetPhotosynthesis Àr CO 2 !Starch Ratesof net photosynthesis (r NetPhotosynthesis ) were calcu- lated as the average rate of carbon uptake during the ﬁrst half of the light phase (n = ... interconversion rates as a function of time of cold exposure, (b) comparison of the magnitudes of the various interconversion rates, and (c) l...

Ngày tải lên : 14/02/2014, 22:20

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Tài liệu Báo cáo khoa học: Neuropeptide Y and osteoblast differentiation – the balance between the neuro-osteogenic network and local control ppt

... and the decreased leptin levels are absent in female mice, suggesting a gender speciﬁcity of the bone response. Further assessment of the role of NPY in the control of bone homeostasis – the ... hypothalamus, the hypothesis that leptin controls bone formation via a central mechanism was raised. The most convincing evidence supporting this hypothesis was the rescue of...

Ngày tải lên : 18/02/2014, 04:20

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Tài liệu Báo cáo khoa học: Toggle switches, pulses and oscillations are intrinsic properties of the Src activation/deactivation cycle doc

... conformation, the SH2 domain binds to pY i on the C-terminal tail, and the Src homology 3 (SH3) domain binds to the linker between the SH2 and kinase domains at the back of the small lobe, preventing the ... presenting the pulse of Src activity in the plane of the inactive and active fractions, s i and s 1 . If the duration of the stimulus exceeds the c...

Ngày tải lên : 18/02/2014, 11:20

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