Báo cáo khoa học: Genomic structure and expression analysis of the RNase j family ortholog gene in the insect Ceratitis capitata pptx

... regulates RNase j expression in C. capitata. Another focus of this work was the study of the exon ⁄ intron structural evolution of the RNase j gene, by means of integration of blast-based analysis ... functional gene was detected in each genome. The intron ⁄ exon organization of the ORF region of the RNase j family genes is shown in Fig....

Ngày tải lên: 23/03/2014, 06:20

... is known about the distribution and regulation of these genes and their responses to hypoxia in mammals, the corres- ponding information in fish is not known. Although a number of GLUT genes have ... Comparative analysis of GLUT genomic structures showed that the arrangement of exons and position of split codons are highly conserved amongst members of the clas...

Ngày tải lên: 17/03/2014, 03:20

... b-Actinwasusedasacontrol of the amount and quality of cDNA. Fig. 4. An unrooted phylogenetic tree constructed by the neighbor join- ing method (using PAUP software) from the amino acid sequences of ... Cloning and analysis of IL-10 in fish (Eur. J. Biochem. 270) 4651 The carp IL-10 harbored two instability motifs, in comparison to five in human IL-10, in the 3¢-...

Ngày tải lên: 20/02/2014, 02:21

... occurred in the discovery of fish cytokine genes. This has been attributed mainly to the enormous progress made in genome projects for the Fugu and zebrafish genome, and the large increase of fish ... c.secombes@abdn.ac.uk Abbreviations: IL, interleukin; IL-1Ra, interleukin 1 receptor antagonist; ICE: interleukin 1b converting enzyme; IL)18 BP, interleukin 18 binding protein;...

Ngày tải lên: 16/03/2014, 16:20

... protein. CD2 peptide mapping and mutagenesis indicated that the binding surface of CD2 consists of b-sheet formed by strands GFCC¢C¢¢.Thecrystal structure of CD2 (Fig. 2B) indicated that the rather ... b-turn structure in solution and clo- sely mimic the b-turn s tructure of the surface epitopes of the CD2 protein. Docking studies of CD2 peptides and CD58 pro...

Ngày tải lên: 19/02/2014, 13:20

... N-terminal chitin-binding domain. ChiG, on the other hand, lacks this chitin-binding domain, and consists only of a catalytic domain [22]. The chiG gene encodes a 244 amino acid chitinase, including ... of chitin, a carbohydrate poly- mer of 1,4-b-linked GlcNAc. Chitin is found in the cuticle of insect shells, in shells of crustaceans, and in the cell walls of...

Ngày tải lên: 07/03/2014, 11:20

... domain, the XPC-binding domain, and two ubiquitin-associated domains (UBA1 and UBA2) [9,10]. The UbL domain has a high bind- ing affinity for polyubiquitin binding site 2 of the human S5a protein, ... amino acids 566–608 and 613–661 of the hHR23B-binding domain of XPC). However, we not able to detect binding of either XPCB–hHR23B or the complete hHR23B protein in GST...

Ngày tải lên: 07/03/2014, 17:20

... pursuing). The structure of the activation domain reported here has allowed us to gain insight into the likely mode of AdoMet binding, and provide atomic level insight into the effect of clinical ... within 4 A ˚ of helix a7, and within 7 A ˚ of Ile1126 in the AdoMet-binding region. In the human structure, the longer a3 helix ends at the beginning of th...

Ngày tải lên: 23/03/2014, 09:21

... helix. Comparison of the tertiary structures including these areas (Fig. 6B) shows that removing the metal ion from the first site determines an opening of the binding loop, and induces important ... of the 17 final structures superimposed based on the main-chain heavy atoms in regular secondary -structure elements. The N-terminal EF-hand domain is shown in red, the...

Ngày tải lên: 23/03/2014, 13:20

... dashed line and the dotted line indicate the elution profile of a further chromatography of the material included in peak A and peak B, respectively. The arrow identifies the broadening of the profile ... reported in Fig. 6B, demonstrate that the protein is still in the oligomeric form, indistinguishable from the protein in the absence of perturbant (Fig....

Ngày tải lên: 23/03/2014, 13:20