Báo cáo khoa học: Roles of adenine anchoring and ion pairing at the coenzyme B12-binding site in diol dehydratase catalysis pptx

... that of the wild-type enzyme at saturating concentrations of KK PDO PDO S224 S224 K135 K135 A BC Fig. 1. The structure of the coenzyme- binding site in diol dehydratase. (A) Stereo drawing of the ... that the oxidation of cob(II)alamin accompanies the inactiva- tion of the Kb135A holoenzyme during catalysis. Upon denaturation of the complex after 30 m...

Ngày tải lên: 23/03/2014, 06:20

... Ets-1 and Egr-1 are substrates of MAPK [12,43,44], the inhibition of MAPK by the incorporation of n-3 PUFAs could alter the phosphorylation of these transcription factors, affecting their binding ... for the regulation of egr-1 gene expression in the chromatin context. The drastic down-regulation of Egr-1 mRNA levels, which is observed in n-3 treated cells, also...

Ngày tải lên: 19/02/2014, 16:20

... the binding, insertion and aggregation of molecules, either at the lipid bilayer surface or upon incorporation into the bilayer. Formation of lipid membranes and peptide incorporation The principles of ... We attribute the aggregation in the DOPC-enriched domain to be a consequence of lipid mixing within these microdomains, resulting in the presence of small...

Ngày tải lên: 30/03/2014, 11:20

... also contains a schematic illustration of the AP-2 structure. Expression patterns of AP-2 molecules and functional implications The expression and function of AP-2 isoforms have been systematically ... helix–span–helix DNA-binding and dimerization domain at their C-terminus, and a less conserved proline-rich and glutamine-rich transactiva- tion domain at their N-termin...

Ngày tải lên: 16/02/2014, 09:20

... effect of HSF4 on chromatin. In the absence of HSF4, histone H3K9 methylation is induced and HSF1 binding is reduced, indicating that HSF4 facilitates HSF1 binding via chromatin remodelling. Heat ... posi- tioning of a coronal section and scheme of the coronal section at E14.5 in (F). (A) At E11.5, HSF2 is expressed in forebrain, midbrain and in hindbrain except...

Ngày tải lên: 18/02/2014, 04:20

... DNA-binding sites, as in RecA [16], and the identification of these sites is important for under- standing the reaction mechanism and the regulation of homologous recombination. So far, the crystal ... should be located, and are not found at the ATP-binding site or the subunit–subunit interface of the Rad51 filament. Therefore, the Rad51 L1 and L2 loops may a...

Ngày tải lên: 19/02/2014, 06:20

... the WalkerA mutation in the other AAA+ module, we tested the roles of the arginines in ATP binding and hydrolysis in each module. The arginine mutations did not affect nucleotide binding to the ... not elucidated. The role of the other conserved arginine (P2) in AAA-1 was also unclear. Here, we investigated in detail the roles of these three arginines...

Ngày tải lên: 06/03/2014, 00:20

... auto-phosphor- ylation by modulating the kinase activity of the enzyme. To determine the roles of the SH3 and ⁄ or SH2 domains in Src regulation of NMDAR phosphoryla- tion, the protein fragment corresponding ... recombinant Src and Fyn may be significantly reduced by application of SH2 domain binding peptides, which results in blocking of the binding of the...

Ngày tải lên: 06/03/2014, 01:20

... the exception of the PCR primers. The primers were 5¢-GCGA ATTC ATGGCATCATATGTAGGA-3¢ and 5¢-CGGA ATTC TTACAACTTTGATAAATATT-3¢ (EcoRI sites in italics; initiation and termination codons underlined). Degradation ... a trace of the pro- tein was left in the unbound fraction. Pre-incubation of the recombinant protein with FP abolished most of this binding, suggesting...

Ngày tải lên: 07/03/2014, 17:20

... MMP cleavage site, functioning as competitive inhibitors, and chelating the zinc ion present at the activation site [58]. On the basis of the group that binds and chelates the zinc ion, peptidomimetis ... to regulate their mobilization, affecting leukocyte in ltration and migration [52,53]. Pharmacological targeting of matrix metalloproteinases On the basis of...

Ngày tải lên: 15/03/2014, 00:20