Báo cáo khoa học: Directed evolution of a histone acetyltransferase – enhancing thermostability, whilst maintaining catalytic activity and substrate specificity doc

... evolution of a histone acetyltransferase – enhancing thermostability, whilst maintaining catalytic activity and substrate specificity Hans Leemhuis 1 , Karl P. Nightingale 1,2 and Florian Hollfelder 1 1 ... that allows the screening of hundreds of histone acetyltransferase mutants for histone acetylating activity, and used this to enhance the ther...

Ngày tải lên: 23/03/2014, 06:20

... succinyl [3] and glutaryl acylases [3,1 1–1 6]. The activity of the glutaryl acylases on substrates with adipyl and a- aminoadipyl side chains varies greatly. As the glutaryl, adipyl and a- aminoadipyl ... The enzyme deacylated glutaryl-7-ACA with a catalytic con- stant k cat of 8.1 s )1 and with a Michaelis constant K m of 0.08 m M . Adipyl-7-ADCA is deacylated at...

Ngày tải lên: 31/03/2014, 09:20

... then assay- ing for activity as described above at concentrations of 0.8 nm DHF and 0.8 nm NADPH. Residual activity was measured by calculating the proportion of initial velocity remaining after ... compounds [12,13], and numerous studies of DHFR from many organisms [11]. The breadth of information available on DHFR and the significance of it in the treatment of a range...

Ngày tải lên: 19/02/2014, 02:20

... 4.1 aaa fi aga Variants of second generation E53V ⁄ K56R ⁄ C23S gaa fi gta 120% a 1600 42 aaa fi aga tgt fi agt a Residual activity was measured in 15% AA ⁄ 0.1% TEMED. Stabilisation of FDH for entrapment ... additional combinations of PAA forming compounds, such as acrylamide ⁄ APS or TEMED ⁄ APS, and higher concentrations of both APS and TEMED were employed. Also, combinations...

Ngày tải lên: 16/03/2014, 13:20

... interface mutation on catalytic activity of triosephosphate isomerase The role of conserved residues and complementary mutations Mousumi Banerjee 1 , Hemalatha Balaram 2 and Padmanabhan Balaram 1 1 ... Structure and function of a regulated archaeal triosephosphate isomerase adapted to high temperature. J Mol Biol 342, 86 1–8 75. 12 Gayathri P, Banerjee M, Vijayalakshmi A,...

Ngày tải lên: 18/02/2014, 11:20

... by invertebrates, and has three crystal phases: calcite, ara- gonite and vaterite. Although calcite is the most stable crystal thermodynamically, many organisms can form metastable aragonite crystals ... region was decreased and a new band was detected at 64 kDa, but only after treatment with heparitinase II (Fig. 5A) . Although the same band was obtained after digestion Fig. 3. 45 Ca...

Ngày tải lên: 18/02/2014, 17:20

... the same time, distinct absorption bands of oxyheme appeared at 540 and 579 nm. Then, a broad band appeared at around 660 nm, and was maximal 9–1 2 min after initiation of the reaction. The spectral ... absorption bands. Heme catabolism by HOs of mammals, pathogenic bacteria, cyanobacteria and probably insects is considered to have a similar mechanism, because the characteri...

Ngày tải lên: 19/02/2014, 05:20

... helices (a9 – a1 0 a1 6 a1 7). Helices a1 2 a1 3 a1 4 a2 0 are placed at the dimer interface. Domain III, residues 43 4–4 69, is assigned as the tail domain. A single helix a2 1, and two short b strands, b9–b10, ... smaller compound PEA (Fig. 1B) and a water molecule are present and PEX and PEA refined satisfactorily with occupancies of 0.7 Table 1. Data and refinement...

Ngày tải lên: 19/02/2014, 05:20

... activities of a catalytic antibody with a peroxidase activity Formation of Fe(II)–RNO complexes and stereoselective oxidation of sulfides Re ´ my Ricoux, Edyta Lukowska, Fabio Pezzotti and Jean-Pierre ... hydroxyl- amines was examined. This constituted a new reaction for hemoabzymes and is the first example of fully characterized Fe(II)–metabolite complexes of antibody...

Ngày tải lên: 19/02/2014, 12:20

... MetLysSerLeuLeuLeuValThrLeuAlaAlaCysPheLeuHisAlaGlySerAla 0 Human MetArg GlyMetLysLeuLeuGlyAlaleuLeuAlaLeuAlaAlaLeuLeuGlnGlyAlaValSer 70 Ó FEBS 2004 Molecular evolution of vertebrate DNase I family (Eur. ... Molecular evolution of shark and other vertebrate DNases I Toshihiro Yasuda 1 , Reiko Iida 2 , Misuzu Ueki 1 , Yoshihiko Kominato 3 , Tamiko Nakajima 3 , Haruo Takeshita 3 , Tak...

Ngày tải lên: 19/02/2014, 16:20