Báo cáo khoa học: Microarray analyses of hypoxia-regulated genes in an aryl hydrocarbon receptor nuclear translocator (Arnt)-dependent manner ppt

... 2008 FEBS Microarray analyses of hypoxia-regulated genes in an aryl hydrocarbon receptor nuclear translocator (Arnt)-dependent manner Su Mi Choi*, Hookeun Oh* and Hyunsung Park Department of Life ... comparing quantitative PCR analyses of these nine genes in BpRc1 and hepa1c1c7 cells, we determined that eight of the nine hypoxia-induced genes are Ar...

Ngày tải lên: 23/03/2014, 06:20

... are involved in the receptor response to chemotactic agonists with the sixth transmembrane domain and the third extracellular loop playing a promin- ent role. Interestingly, the N-terminus and ... pep- tide receptors were incubated with a constant concentration of 125 I-labeled W pep in the presence of increasing concentrations of unlabeled ligand. After incubation cell pellets we...

Ngày tải lên: 16/03/2014, 18:20

... (middle and right panels). (B) Growth curves of HHY212s strains harboring only the heIF5A proteins in YPGal. Wes- tern blot analyses of proteins of HHY212d strains harboring both recombinant plasmids, ... Blue staining of BL21(DE3) lysates expressing human mutant proteins (A, top panel) and fluorogram of the SDS gel of DHS ⁄ DOHH reaction mixtures showing labeling of several...

Ngày tải lên: 16/03/2014, 06:20

... 6A). An equal amount of protein content for each lane was verified by Coomassie staining (Fig. 6B). PP13/galectin-13 binds annexin II and beta/gamma actin By Coomassie staining after SDS/PAGE, in ... (1992) Human brain lectin: a soluble lectin that binds actin. J. Neurochem. 58, 200–203. 51. Hughes, R.C. (1999) Secretion of the galectin family of mamma- lian carbohydrate-binding prot...

Ngày tải lên: 23/03/2014, 12:20

... (2007) Computational analysis and identification of amino acid sites in dengue E proteins relevant to developments of diagnostics and vaccines. Virus Genes 35, 175–186. 4 Dussart P, Petit L, Labeau B, Bremand ... transcription-PCR assay for detecting and typing dengue viruses with GC tail and induced fluorescence resonance energy transfer techniques for melting temperature and color mul...

Ngày tải lên: 14/02/2014, 19:20

... post-transfection by maintaining the cells in culture medium containing 2% horse serum. Cells were fixed on day 4 of differentiation and stained with hematoxylin and eosin. One of two independent ... regulator of energy metabolism in the fat body of fruit flies [3]. In vertebrates, miR- 375 and miR-376, both of which are abundantly expressed in pancreatic b-cells, are involved...

Ngày tải lên: 18/02/2014, 08:20

... interact with a number of functional groups, including the aromatic side chains of some amino acids, through a stacking mechanism [42]. The interaction of arginine with aromatic amino acids of aB-crystallin ... of aB-crystallin. Reduction-induced aggregation of the insulin B-chain Light scattering due to DTT-induced amorphous aggregation and precipitation of the insulin B-chain...

Ngày tải lên: 18/02/2014, 16:20

... November 2006) doi:10.1111/j.1742-4658.2006.05579.x Chemokines elicit their function by binding receptors of the G-protein-cou- pled receptor class, and the N-terminal domain (N-domain) of the receptor is one of the two critical ligand-binding sites. ... show a remarkable range of receptor selec- tion and function, with some binding a single receptor, some binding multiple rec...

Ngày tải lên: 19/02/2014, 05:20

... (rotation angle ‘r’) and variation of the inclination (inclination angle ‘i’) with respect to the plane of the membrane (parallel to the xy plane). Cter and Nter are the C-terminal and N-terminal structural ... proteins involved in signal transduction and vesicle trafficking, specifically associate to negatively charged membranes. As all these data are suggestive of a role of mem- bra...

Ngày tải lên: 19/02/2014, 06:20

... extension to the protein kinase folds back on the kinase domain and interferes with the substrate binding sites. In the case of the titin and twitchin kinases, the autoinhibitory sequence acts ... consisting of four copies of four subunits, a, b, c and d. The a and b subunits are regu- latory and are the sites of phosphorylation and meta- bolite binding and are also regulated by the...

Ngày tải lên: 19/02/2014, 17:20