Báo cáo khoa học: Microarray analyses of hypoxia-regulated genes in an aryl hydrocarbon receptor nuclear translocator (Arnt)-dependent manner ppt

... 2008 FEBS Microarray analyses of hypoxia-regulated genes in an aryl hydrocarbon receptor nuclear translocator (Arnt)-dependent manner Su Mi Choi*, Hookeun Oh* and Hyunsung ParkDepartment of Life ... comparingquantitative PCR analyses of these nine genes in BpRc1 and hepa1c1c7cells, we determined that eight of the nine hypoxia-induced genes are Arntdependent. Additional quantitative PCR analyses ... activation of the PKR-like ER kinase (PERK), suppression of translation andinduction of several transcription factors and chaper-one proteins, including activating transcription factor-4(ATF-4) and...

Báo cáo khoa học: Identiﬁcation of functional domains in the formyl peptide receptor-like 1 for agonist-induced cell chemotaxis doc

... areinvolved in the receptor response to chemotactic agonists with the sixthtransmembrane domain and the third extracellular loop playing a promin-ent role. Interestingly, the N-terminus and ... pep-tide receptors were incubated with a constant concentration of 125I-labeled W pep in the presence of increasing concentrations of unlabeled ligand. After incubation cell pellets were collected andmeasured ... FEBSthe involvement of multiple domains of FPR in recogni-tion of the agonist fMLF, including the ﬁrst, second,and third extracellular loops. The TMs in FPR are alsoimplicated in contributing...

Báo cáo khoa học: Mutational analyses of human eIF5A-1 – identiﬁcation of amino acid residues critical for eIF5A activity and hypusine modiﬁcation doc

... (middle and right panels). (B) Growth curves of HHY212s strains harboring only the heIF5A proteins in YPGal. Wes-tern blot analyses of proteins of HHY212d strains harboring both recombinant plasmids, ... Blue staining of BL21(DE3) lysates expressing human mutant proteins (A, top panel)and ﬂuorogram of the SDS gel of DHS ⁄ DOHH reaction mixturesshowing labeling of several mutant proteins (A, ... deoxyhypusine-containingFig. 2. Growth analysis of S. cerevisiae strains expressing heIF5A wild-type and mutant proteins (A, B) and stability of heIF5A proteins (C,D). (A) The haploid strain HHY13a was transformed...

Báo cáo khóa học: Functional analyses of placental protein 13/galectin-13 pdf

... 6A). An equal amount of protein content for each lanewas veriﬁed by Coomassie staining (Fig. 6B).PP13/galectin-13 binds annexin II and beta/gamma actinBy Coomassie staining after SDS/PAGE, in ... (1992) Human brain lectin: a soluble lectin that bindsactin. J. Neurochem. 58, 200–203.51. Hughes, R.C. (1999) Secretion of the galectin family of mamma-lian carbohydrate-binding proteins. Biochim. ... lysophospholipaseactivity of both the puriﬁed and also the recombinantprotein was veriﬁed. Sugar binding assays revealed thatN-acetyl-lactosamine, mannose and N-acetyl-glucosamineresidues widely expressed in human...

Tài liệu Báo cáo khoa học: PCR detection of nearly any dengue virus strain using a highly sensitive primer ‘cocktail’ ppt

... (2007)Computational analysis and identiﬁcation of amino acidsites in dengue E proteins relevant to developments of diagnostics and vaccines. Virus Genes 35, 175–186.4 Dussart P, Petit L, Labeau B, Bremand ... transcription-PCRassay for detecting and typing dengue viruses with GCtail and induced ﬂuorescence resonance energy transfertechniques for melting temperature and color multiplex-ing. Clin ... reconsidered: the meaning of these terms in analytical and diagnostic settings. Ann Intern Med126, 91–94.20 Yin Z, Chen Y-L, Schul W, Wang Q-Y, Gu F, Durai-swamy J, Kondreddi RR, Niyomrattanakit P,...

Tài liệu Báo cáo khoa học: A role of miR-27 in the regulation of adipogenesis ppt

... post-transfection by maintaining the cells in culture medium containing 2% horse serum. Cells were ﬁxed on day 4 of differentiation and stained with hematoxylin and eosin. One of two independent ... regulator of energy metabolism in the fat body of fruit ﬂies [3]. In vertebrates, miR-375 and miR-376, both of which are abundantlyexpressed in pancreatic b-cells, are involved in the con-trol of insulin ... miR-27 resulted in blockade of expression of PPARc andC ⁄ EBPa, the two master regulators of adipogenesis. Importantly, expression of miR-27 was increased in fat tissue of obese mice and was regulated...

Tài liệu Báo cáo khoa học: The effect of small molecules in modulating the chaperone activity of aB-crystallin against ordered and disordered protein aggregation pdf

... interact with a number of functionalgroups, including the aromatic side chains of someamino acids, through a stacking mechanism [42]. Theinteraction of arginine with aromatic amino acids of aB-crystallin ... of aB-crystallin.Reduction-induced aggregation of the insulin B-chainLight scattering due to DTT-induced amorphousaggregation and precipitation of the insulin B-chaincommenced after 10 min ... activity of aB-crystal-lin against the DTT-induced aggregation and precipi-tation of insulin.ACBFig. 2. aB-crystallin protects against theDTT-induced aggregation of insulin, and thisactivity...

Tài liệu Báo cáo khoa học: Thermodynamic characterization of interleukin-8 monomer binding to CXCR1 receptor N-terminal domain ppt

... November2006)doi:10.1111/j.1742-4658.2006.05579.xChemokines elicit their function by binding receptors of the G-protein-cou-pled receptor class, and the N-terminal domain (N-domain) of the receptor is one of the two critical ligand-binding sites. ... show a remarkable range of receptor selec-tion and function, with some binding a single receptor, some binding multiple receptors, and some bindingone receptor with high afﬁnity and others with ... buried predominantly because of intermolecular interactions (binding) and not because of intramolecular interactions (folding). Small chan-ges in the thermodynamic parameters on folding alsosuggest...

Tài liệu Báo cáo khoa học: The role of electrostatic interactions in the antitumor activity of dimeric RNases docx

... (rotationangle ‘r’) and variation of the inclination (inclination angle ‘i’) withrespect to the plane of the membrane (parallel to the xy plane).Cter and Nter are the C-terminal and N-terminal structural ... proteins involved in signal transduction and vesicle trafﬁcking, speciﬁcallyassociate to negatively charged membranes.As all these data are suggestive of a role of mem-branes, in particular of ... eventually permeating membranes,and that intracellular membranes play a decisive role in deﬁning the antitumor action of an RNase.Experimental proceduresAssays with membranesSynthetic DMPG and DMPS...

Tài liệu Báo cáo khoa học: Structural characterization of Ca2+/CaM in complex with the phosphorylase kinase PhK5 peptide pdf

... extension to the protein kinase folds backon the kinase domain and interferes with the substratebinding sites. In the case of the titin and twitchinkinases, the autoinhibitory sequence acts ... consisting of four copies of foursubunits, a, b, c and d. The a and b subunits are regu-latory and are the sites of phosphorylation and meta-bolite binding and are also regulated by the binding of ... phosphorylase kinase throughthe combined action of calmodulin and troponin. AnnNY Acad Sci 356 , 151–161.4 Manning G, Whyte DB, Martinez R, Hunter T &Sudarsanam S (2002) The protein kinase complement...

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