... reported [12].Therefore, inactivation of Tm7sf2 gene in mice wouldprovide insights into the role of both genes encoding 3b-hydroxysterol D14-reductase, Tm7sf2 and Lbr ,in cholesterol biosynthesis. While ... fragment,containing exons 1–5 of the gene (Fig. 2B). The neomycinphosphotransferase gene (neo) was inserted in a SalIrestriction site generated by in vitro mutagenesis in exon 5. The vector was linearized ... Journal compilation ª 2008 FEBS 5047 Disruption of the gene encoding 3b-hydroxysterol D14-reductase (Tm7sf2) in mice does not impair cholesterol biosynthesis Anna M. Bennati1, Gianluca Schiavoni1,...