Báo cáo khoa học: Disruption of the gene encoding 3b-hydroxysterol D14-reductase (Tm7sf2) in mice does not impair cholesterol biosynthesis pdf

... reported [12]. Therefore, inactivation of Tm7sf2 gene in mice would provide insights into the role of both genes encoding 3b-hydroxysterol D 14 -reductase, Tm7sf2 and Lbr ,in cholesterol biosynthesis. While ... fragment, containing exons 1–5 of the gene (Fig. 2B). The neomycin phosphotransferase gene (neo) was inserted in a SalI restriction site generated by...

Ngày tải lên: 23/03/2014, 06:20

... subpopulations of enzyme with their ISP in different conformations. The mutation G167E could distort the hinge region of the ISP and hinder the movement of the headgroup Mutations in the hinge region of the ... to the hingeregion mutations, and we suggest that G167E hinders the movement of the ISP by altering the ISP–cytochrome b interaction at the hinge...

Ngày tải lên: 07/03/2014, 15:20

... the main nitrogen compounds in the phloem exudates, amounting to 51% of the total amino acids in the light and 56% in the dark (Fig. 7A). The total amount of alanine, serine and gly- cine was ... 33% in the light to 25% in the dark (Fig. 7A). Glutamine and asparagine were the major amino acids in the xylem sap in both the light (63% of the total ami...

Ngày tải lên: 18/02/2014, 18:20

... suggested a single cut at the C9–C10 double bond of the ring-bearing side of 3-OH-c-carotene in combination with several cleavage options in the linear half of the molecule, namely at the C9¢–C10¢,C7¢–C8¢ ... temperature of 340 °C, which was maintained for an addi- tional 5 min. The He carrier gas flow was maintained at 1mLÆmin )1 using a split flow of 1 : 20. The sp...

Ngày tải lên: 07/03/2014, 03:20

... arrows). Owing to the horizontal smear, however, the presence of the B14 subunit in the lower band cannot be ascer- tained at this point. A cross-reaction of the antibody against the 39 kDa subunit of ... oligomer of the small form. The different in uences of DPI on the two forms could be caused by its failure to access the flavin cofactor in the large form...

Ngày tải lên: 07/03/2014, 09:20

... the Gibbs energy of unfolding at 0 [urea] (DG U0 ). In the mutant series, the overall height of the energy barrier for the rate-determining step of the fold- ing ⁄ unfolding transition is mainly ... near the crossing region of the helices, and the N x atom of K8 is found at a distance of 6.2 A ˚ from the nearest side-chain oxy- gen of D72. Taking the func...

Ngày tải lên: 30/03/2014, 20:20

... component of the cell–cell adhesion complex and required for the for- mation of epithelia in the embryo and the maintenance of the polarized epithelial structure in the adult [2]. As a single-span ... Grb2 (Fig. 5B). Interestingly, Src inhibition also suppressed the disruption of cell–cell adhesion, the scattered phenotype of the cells and the redistributio...

Ngày tải lên: 19/02/2014, 05:20

... binding factors. The introduction of mutation in one of the Ets-binding sequences greatly decreased its promoter activity, suggesting that the transcription of the a3 integrin gene in these cells ... products in the absence of the cDNA library. Fig. 6. Transcription start sites in the integrin a subunit genes. The sequences flanking the transcription start s...

Ngày tải lên: 21/02/2014, 03:20

... detailed mechanisms involved in regulating the activity of individual gene promoters [42]. We are interested in und erstanding the transcriptional regulation of genes encoding essential components of the mitochondrial ... transfection into Schneider SL2 cells. A series of deletions of the 5¢ upstream region of the gene were cloned in the pXp2 vector that co...

Ngày tải lên: 07/03/2014, 16:20

... components of eel egg envelope. The protein names deter- mined after sequence analyses of the cDNAs are indicated in parentheses. The numbers in parentheses at the end of each sequence indicate the ... and the ovary in Otocephala; an excep- tion is the gilthead seabream, where the glycoproteins originate from both the liver and ovary. However, the genes encodi...

Ngày tải lên: 15/03/2014, 23:20