Báo cáo khoa học: TioS T-TE – a prototypical thioesterase responsible for cyclodimerization of the quinoline- and quinoxaline-type class of chromodepsipeptides potx

... the first in vitro char- acterization of a prototypical thioesterase responsible for the iterative assembly of the quinoline- and quinox- aline-type class of chromodepsipeptides, capable of macrolactonization ... for the biocombinatorial synthesis of the quinoline- and quinoxaline-type class of chromodepsipeptides. By con- trast to linearly ope...

Ngày tải lên: 23/03/2014, 06:20

... cerevisiae DNA was used as a PCR template for PCR. For construction of pUG35-LPX1 (LPX1–GFP), PCR-amplified YOR084w (primers 5¢-GCTCTAGAATG GAACAGAACAGGTTCAAG-3¢ and 5¢-CGGAATTCCA GTTTTTGTTTAGTCGTTTTAAC-3¢) ... primers 5¢-CCCGGGAAT GGAACAGAACAGGTTCAAG-3¢ and 5¢-AGATCTTTA CAGTTTTTGTTTAGTCGTTTT-3¢, and introduced into pGEM-T (Promega, Mannheim, Germany). The ORF was excised using...

Ngày tải lên: 07/03/2014, 05:20

... primer-pairs: rpS7-forward 5¢-CGAAGTTGGTCGG-3¢ and rpS7-reverse 5¢-GGGAATTCAAAATTAACATCC-3¢; b-actin control specific primer pairs: b-actin-forward 5¢-TGTTACCAACT GGGACGACA-3¢ and b-actin-reverse 5¢-AAGGAAGGC TGGAAAAGAGC-3¢. ... splice variants, as well as biochemical characterization of the canonical alfa isoform (mSMOa), have been reported recently [10,11]. The subcellular localiza...

Ngày tải lên: 07/03/2014, 17:20

... mutagenesis kit (Stratagene, La Jolla, CA, USA) and the primers AkpnI: 5¢-GATACAT GGTATCGTCCAAAAC-3¢; AsacI: 5¢-GTG AAGAAATAT GAGCTCTATAATATTCCGG-3¢; and Asa lI: 5¢-CTTGCTAACGAT GTCGACAACTCTAATCC-3¢ ... transglucosylation and hydro- lysis activity ratios. Application of directed evolution strategies, using random and rational mutagenesis approaches, has allowed conversion of CGT...

Ngày tải lên: 16/03/2014, 04:20

... (GE Healthcare, Anapolis, MD, USA) to the ICU but not on the gen- eral wards. The new system was introduced following a pro- gram of staff training and HWP was completely changed on a single day. The ... investigator illness. The ICU medical and nurs- ing staff were unaware that the study was being conducted. Ethical approval was not sought, because at the time audits...

Ngày tải lên: 25/10/2012, 10:39

... Boer T, Hazelaar MJ, Anazawa H, Harms N, Oltmann LF & Stouthamer AH (1991) A method for introduction of unmarked mutations in the genome of Paracoccus denitrificans – construction of strains with ... demonstrated that Asp129 of NirF could not be essential for any function similar to that in Asp141 of Met8P. The idea of NirF being a dehydrogenase is appealing bec...

Ngày tải lên: 15/02/2014, 01:20

... to cathepsin H. To illustrate this, we calculated the aver- age distances between CA atoms of the active site cys- teine and histidine residues in cathepsins B and H and the center of CA atoms of ... structure of the occluding loop residues adapts to each binding ligand in its own way and swings out only as much as is mandatory. Results and Discussion Crystals of...

Ngày tải lên: 18/02/2014, 04:20

... Petersburg, FL, USA) followed by PCR with Taq DNA polymerase (Promega, Madison, WT, USA) using the primers 5¢-CACACTACACTGGGAAGCAGAG ACTCCAGC-3¢ and 5¢-AGCTGGCCCAGCAGCCATACACAGTTAAAG- 3¢. The cDNA was subcloned ... Tomohiro Chiba for preparation of embryonic day 9.5 mouse embryos; Dr Dovie Wylie and Ms Tak- ako Hiraki for expert assistance; and all members of the Departmen...

Ngày tải lên: 18/02/2014, 16:20

... have shown that redefining the task of lemma- tisation as a category tagging task and using an ef- ficient tagger to perform it results in a performance that is at the state -of- the- art level. The ... lemma) pairs: (nedok ´ azal, dok ´ azat), (neexistoval, existovat), (nepamatoval, pamatovat). 1 The set of labels for a specific language is induced from a training set...

Ngày tải lên: 19/02/2014, 19:20

... generalization accuracy. Semi-supervised models such as Ando and Zhang (2005), Suzuki and Isozaki (2008), and Suzuki et al. (2009) achieve state -of- the- art accuracy. However, these approaches ... results Table 2 shows the final chunking results and Ta- ble 3 shows the final NER F1 results. We compare to the state -of- the- art methods of Ando and Zhang (2005), Suzuki and...

Ngày tải lên: 20/02/2014, 04:20