Báo cáo khoa học: Cytokinin-induced structural adaptability of a Lupinus luteus PR-10 protein potx
Tài liệu Báo cáo khoa học: Novel aggregate formation of a frame-shift mutant protein of tissue-nonspecific alkaline phosphatase is ascribed to three cysteine residues in the C-terminal extension pdf
... Michigami T, Yamamoto T, Yasui N, Satomura K, Yamagata M, Shima M, Nakajima S, Mushiake S, Okada S & Ozono K (1998) Analysis of localization of mutated tissue-nonspecific alkaline phosphatase proteins associated ... fraction). BSA (b, 68 kDa), alcohol dehydrogenase (a, 141 kDa) and catalase (250 kDa) were loaded on to a separate gradient as mole- cular mass markers. K. Komaru et al....
Ngày tải lên: 19/02/2014, 17:20
Báo cáo khoa học: Kinetically controlled refolding of a heat-denatured hyperthermostable protein pot
... process of LamA was moni- tored using calorimetry. Analysis of the thermograms Fig. 4. Enzymatic activity of native and heat-treated LamA at differ- ent storage times. The activity was measured at ... the maintenance of protein structural integ- rity and function at elevated temperatures [2,3]. For the stabilization of proteins at high temperatures, a concerted optimization o...
Ngày tải lên: 07/03/2014, 05:20
Báo cáo khoa học: Isolation and characterization of a D-cysteine desulfhydrase protein from Arabidopsis thaliana pptx
... l-amino acids such as amino acid oxidases, transaminases, and racemases (epimerases). For example, in pea seedlings the occurrence of d-amino acid aminotransferase was demonstrated [36]. For a ... Ohkishi H, Kawakami B, Yamano H, Hosono H, Tani Y & Yamada H (1982) 3-chloro-d-ala- nine chloride-lyase (deaminating) of Pseudomonas putida CR 1–1. Purification and characterization of a...
Ngày tải lên: 16/03/2014, 18:20
Báo cáo khoa học: Efficient synthesis of a disulfide-containing protein through a batch cell-free system from wheat germ pdf
... region was constructed by ampli- fying the whole plasmid pEU-scFvLH by inverse PCR with the primers s2: 5¢- CAAAAAATTGAATGGCATG AACCGCCGAGCTCCAAC-3¢ and a2 : 5¢-AGCTTCAA AAATATCATTTAAACCCGACGGGCTGCTTTT-3¢ (the ... synthesis of a disulfide-containing protein through a batch cell-free system from wheat germ Takayasu Kawasaki 1 , Mudeppa D. Gouda 1 , Tatsuya Sawasaki 1,2 , Kazuyuki Takai...
Ngày tải lên: 16/03/2014, 23:20
Báo cáo khoa học: Cytokinin-induced structural adaptability of a Lupinus luteus PR-10 protein potx
... model. Structural comparisons of PR-10 proteins All the known models of PR-10 proteins have a com- mon canonical fold, consisting of a seven-stranded antiparallel b-sheet wrapped around a long ... conformational changes accompanying ligand binding. The structural adaptability of LlPR-10.2B and its ability to bind different cytokinins suggest that this protein, and per...
Ngày tải lên: 23/03/2014, 06:20
Tài liệu Báo cáo khoa học: NMR structural characterization of HIV-1 virus protein U cytoplasmic domain in the presence of dodecylphosphatidylcholine micelles doc
... structure of micelle-associated VpUcyt was cal- culated on the basis of the final set of proton–proton dis- tance constraints using cyana, a software program that combines simulated annealing with ... introduction of paramagnetic probes into the interior of the micelle or the aqueous buffer. Qualitative analysis of secondary chemical shift and paramagnetic relaxation enhancement...
Ngày tải lên: 18/02/2014, 06:20
Báo cáo khoa học: The structural basis of calpain behavior pptx
... (l-cal- pain or calpain 1) and milli-calpain (m-calpain or calpain 2), have been the focus of three decades of intensive characterization. In vitro analysis has shown that the Ca 2+ concentration ... stars). This Ca 2+ -free configuration corresponds to a structurally inactive conformation of calpain 2. DIII is related to the spatial organization of the C2 domain and binds Ca 2+ ions...
Ngày tải lên: 23/03/2014, 10:21
Báo cáo khoa học: The structural comparison of the bacterial PepX and human DPP-IV reveals sites for the design of inhibitors of PepX activity pot
... against the X-PDAP of pathogens would probably affect the activity of the mammalian enzymes, leading to harmful consequences for human health. Taking advantage of the structural differences and ... 1.70 a Tests carried out on DPP-IV to validate the computational protocol of AUTODOCK. b Mean of values calculated by AUTODOCK 3.0. c Mean of the rmsd calculated between the solutio...
Ngày tải lên: 23/03/2014, 13:20
Tài liệu Báo cáo khoa học: C-Terminal extension of a plant cysteine protease modulates proteolytic activity through a partial inhibitory mechanism doc
... CT ex 114 aa 208 aa 24 aa BamH 34 aa Start Stop Xho1 N -Pro Protease domain Protease domain CT - ex 380 aa 1 II NP 114 aa 208 aa BamH1 34 aa Start Stop Xho1 SS SS G SSS S N-Pro 356 aa III MSTLFII S ILLFLAS F SYAMDI S TIEYKYDKSS AWRTDEEVKEIYELWLAKHDKVY SG LVEYEKRFEIFKDNLKFIDEH NSENHTYKMGLTPYTDLTNEEFQAIYLGTRSDTIHRLKRTINISERYAYEAGDNLPEQIDWRKKGAVTPVKNQGKCG SCWAFSTVSTVESINQIRTGNLISLSEQQLVDCNKKNHG...
Ngày tải lên: 14/02/2014, 14:20
Tài liệu Báo cáo khoa học: Intrinsic GTPase activity of a bacterial twin-arginine translocation proofreading chaperone induced by domain swapping ppt
... TorD GTPase activity (Fig. 1B). Titration of increasing amounts of MgCl 2 gradually enhances GTPase activity, with a peak at approximately 1 mm (Fig. 1B). The malachite green assay was also performed ... TorD GTPase activity can be isolated by Cibacron Blue affin- ity chromatography. (A) Unusual behaviour of TorD on CibacronÔ Blue affinity media. A sample of 0.5 m M metal affinity ch...
Ngày tải lên: 16/02/2014, 09:20