Báo cáo khoa học: The multi-replication protein A (RPA) system – a new perspective ppt

... 5¢-AAATATAAAACGCTAGCGTCGACATGGC GC-3¢ and 5¢-AGCGTAAAGGATGGGGAAAG-3 ¢. The final ratio of target cells was determined by the number of colonies retaining the target gene divided by that of total ... recombination at the HOP2 promoter region was amplified from MC-F1 genomic DNA using primers 5¢-AAAAGCGGCCGCTTAAAGCAAGGGTAA ATT-3¢ and 5¢-TTTTGAGCTCATCTTTCAAATAGAGC CTGG -3¢, and inserted in...

Ngày tải lên: 22/03/2014, 21:20

... mutants simulating disease resistance response. Cell 77, 56 5–5 77. 26 Takahashi A, Agrawal GK, Yamazaki M, Onosato K, Miyao A, Kawasaki T, Shimamoto K & Hirochika H (2007) Rice Pti 1a negatively ... buffer and [c- 32 P]-ATP. MBP was used as an artifi- cial substrate to assess the kinase activity and GST alone was used as a negative control. The top panel shows the kinase assa...

Ngày tải lên: 14/02/2014, 19:20

... as a part of Gag, as an unassembled protein, and as an independent protein forming the mature capsid. Remarkably, the CA poly- peptide appears to have evolved an extraordinary con- formational ... exten- sive thermodynamic characterization of the dimerization domain of the HIV-1 capsid protein. Protein Sci 14, 238 7–2 404. 52 Alcaraz LA, del Alamo M, Barrera FN, Mateu MG &am...

Ngày tải lên: 18/02/2014, 06:20

... del Carmen Lidon-Moya and Estefanı ´ a Hurtado- Go ´ mez are acknowledged for their careful experimental work. May Garcı ´ a, Marı ´ a del Carmen Fuster, Javier Casanova and Olga Ruiz de los Pan ˜ os ... private Foundation (Exp: 36557⁄ 06) and Generalitat Valenci- ana (ACOMP ⁄ 2009⁄ 185). Drs Mauricio G. Mateu and Francisco N. Barrera are thanked for critically reading the manuscript a...

Ngày tải lên: 18/02/2014, 06:20

... which carry an RNase domain for dsRNA cleavage and pro- cessing [1 2–1 4]. A second group of dsRBM-containing proteins do not carry an identifiable catalytic domain. Examples are the staufen and Xlrbpa ... categories depending on whether the protein also harbours a catalytic domain [6]. A part- nership with a catalytic domain is observed in the RNA-dependent protein kinase...

Ngày tải lên: 19/02/2014, 17:20

... receptor subunit mutations are both sensitive and resistant to volatile anesthetics. Anesth Analges 95, 57 8–5 82. 38 Takasaki M, Tatara T, Suezaki Y, Shirahama K, Kamaya H, Ueda I & Totoki T (1991) ... alcohols. Statistical quality Although the focus of the mathematical approach is on the ‘leftward shift’, illustrated in Figs 1 and 2, a statisti- cal analysis was performed for...

Ngày tải lên: 19/02/2014, 18:20

... KdpD inserted into the membrane as judged by the appearance of the C-terminal fragment (lanes 2 and 5). As a control, the accumulation of M13 procoat protein was analysed in a parallel culture (Fig. ... immuno- precipitated with antibodies to the respective tags (anti-HA or anti-T7) and analysed by SDS/PAGE, and the bands were visualized on a phosphorimager. The transloc...

Ngày tải lên: 20/02/2014, 11:20

... If the element in the model that was the denotation of the h-expression was not expanded in the same way as the image in this table, a new element corresponding to the expanded image is added ... information that is needed to determine the meanings of the sentences actually presented to the system. Dynamic model-theoretic semantics allows the evaluation of a...

Ngày tải lên: 21/02/2014, 20:20

... pocket on the protein sur- face, is the binding site of CsA and its analogs. Muta- tional analyses and co-crystallization data have isolated residues 87 HAGPIA 92 in CA, nicknamed the cyclophilin ... incorpo- rated into virions and their ability to undergo aminoa- cylation [11]. Because the aminoacylation defect of these tRNA variants was primarily in the K m parame- ter, it was s...

Ngày tải lên: 07/03/2014, 00:20

... TGGACGGATATTGAAGCTGATCTCACCATAAAGGAT Vps4–DEL R ATCCTTTATGGTGAGATCAGCTTCAATATCCGTCCA Vps4–TRP F TTAAAGGCTATCAAATCGCAAGAACAGTTCACTAGA Vps4–TRP R TCTAGTGAACTGTTCTTGCGATTTGATAGCCTTTAA Vps4–RDF F GAAGCAAGAACAGTTCACTTAGTCAATTGATTAACGTG Vps4–RDF ... 11, 22 7– 239. 16 Fujita H, Yamanaka M, Imamura K, Tanaka Y, Nara A, Yoshimori T, Yokota S & Himeno M (2003) A dominant negative form of the...

Ngày tải lên: 07/03/2014, 05:20