Báo cáo khoa học: Functional role of fumarate site Glu59 involved in allosteric regulation and subunit–subunit interaction of human mitochondrial NAD(P)+-dependent malic enzyme pptx

... compilation ª 2009 FEBS Functional role of fumarate site Glu59 involved in allosteric regulation and subunit–subunit interaction of human mitochondrial NAD(P) + -dependent malic enzyme Ju-Yi Hsieh 1, *, ... site for human m-NAD-ME. (A) Homotetramer of human m-NAD-ME (Proein Data Bank code: 1PJ3). (B) Fumarate- binding site of m-NAD-ME. The cor...

Ngày tải lên: 23/03/2014, 06:20

... ‘linker region’ of $ 75 amino acids that connects two homologous halves, each of which contain a transmembrane domain followed by a nucleotide-binding domain. To investigate the role of the linker ... activity in mutants of the human multidrug resistance protein. J Biol Chem 271, 1877–1883. 31 Loo TW & Clarke DM (1999) Identification of residues in the drug-binding domain...

Ngày tải lên: 29/03/2014, 23:20

... polymerization sites of fibrin monomers. Site ‘A’ (Aa17–19) is exposed in the N-terminal region of the Aa-chain by the splitting off of fibrinopeptide A [6]. Site ‘a’ is formed by amino acid residues ... polymerization of fibrin obtained from recombinant fibrinogen with histidine substituted for arginine at Bb14. Turbidity analysis showed an increase in lag-time and a decrease...

Ngày tải lên: 30/03/2014, 03:20

... activating enzyme (E1), a conjugating enzyme (E2) and a ligase (E3) (reviewed in [14]). Of the three enzymes, E3 enzymes are the key determining factors in substrate protein selection. Synoviolin, ... Synoviolin is involved in the degradation of PS cofactors using syno- violin-null cells, as PS cofactors undergo the ubiqui- tin ⁄ proteasome pathway. We report that Synoviolin...

Ngày tải lên: 23/03/2014, 04:20

... b-ketothiolase; PaaZ, unknown, putative ring-cleavage enzyme, aldehyde dehydrogenase domain (N-terminus), MaoC-like protein domain (C-terminus). PaaX and PaaY may be involved in regulation. 3048 W. Ismail ... several proteobacteria, including E. coli, Pseudomonas putida and Azoarcus evansii, contain clusters of 11–16 genes believed to be involved in the catabolism of phenyl...

Ngày tải lên: 23/03/2014, 18:20

... moderate staining in the interstitium (purple arrows). (C–E) Staining of sPLA 2 -V in three active RA tissues. In all cases, intense staining of the granulation tissue in the sublining interstitium ... staining of sPLA 2 -IIE was observed in two inactive RA sections (Fig. 4A), it was intense in VSMC in three distinct active RA sections (Fig. 4Ba– c). In contrast, stainin...

Ngày tải lên: 19/02/2014, 16:20

... (2003) Regulation of the phosphatidylinositol 3- kinase, Akt ⁄ protein kinase B, FRAP ⁄ mammalian target of rapamycin, and ribosomal S6 kinase 1 signaling path- ways by thyroid-stimulating hormone ... The luteinizing hormone-releasing hor- mone inhibits the anti-apoptotic activity of insulin-like growth factor-1 in pituitary aT3 cells by protein kinase Ca-mediated negative regula...

Ngày tải lên: 16/03/2014, 05:20

... V max , K m and K i values of enzymes involved in the adenine and hypoxanthine metabolism in yeast cytosol. V max values represent the average of a minimum of three determinations obtained from ... from Fig. 1 and Table 1, suggested that (a) the main if not unique source of inosine is the intracellular pool of adenine nucleotides, (b) the decrease in ATP and the incr...

Ngày tải lên: 17/03/2014, 10:20

... GALDH in plants lacks the histidine residue involved in covalent flavinylation in GUO, ALO and GLO, but contains a leucine resi- due instead (Leu56 in mature AtGALDH), indicating that the flavin cofactor ... cleavage site of the mitochondrial targeting sequence in plant GALDH (FR ⁄ YA). The asterisk (*) marks the histidine residue involved in covalent binding of the FA...

Ngày tải lên: 23/03/2014, 07:20

... the amounts of pro-b-conglycinin and proglycinin are considered to be nearly equivalent to the synthesis levels of both b-conglycinin and glycinin. The synthesis of proglycinin was initiated when ... with His-Bind resin (EMD Biosciences, Inc.). After washing of the column with binding buffer containing 60 mm imidazole, recombinant proteins were eluted with binding buffer containin...

Ngày tải lên: 23/03/2014, 09:21