Báo cáo khoa học: Cholinoceptive and cholinergic properties of cardiomyocytes involving an amplification mechanism for vagal efferent effects in sparsely innervated ventricular myocardium ppt

... can also exert an important in uence in determining the electronic structure of the haem pocket. To extend our understanding of the significance of proximal characteristics in regulating haem properties ... characterization of mutants [2–4] designed to probe various aspects of its catalytic mechanism and spectroscopic properties. Detailed structural informa- tion for...

Ngày tải lên: 07/03/2014, 21:20

... each band of interest, and an identical rectangle was used in each lane for background subtraction. Results Recombinant expression of human anionic trypsinogen The gene for human anionic trypsinogen ... 15-kDa band, containing both the N- and C-terminal chains that are identical in size (band A). Activation of double-chain trypsinogen to double-chain trypsin results in t...

Ngày tải lên: 17/03/2014, 03:20

... humans, angiogenin (RNase 5) is an inducer of neovascularization, and plays an important role in tumor growth [5]. Eosinophil-derived neurotoxin (RNase 2) and eosinophil cationic protein (RNase ... resonance studies of the structure and binding sites of enzymes. 8. Inhibitor binding to ribonucleases. J Mol Biol 45, 491–511. 33 Ui N (1971) Isoelectric points and conformatio...

Ngày tải lên: 14/02/2014, 22:20

... [1]. The transfer of phosphate can lead to phosphorylation of inosine, guanosine and a number of their analogues [2]. There- fore, in addition to being involved in regulation of purine intracellular ... 328, 483–487. 20 Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein uti- lizing the principle of protein–dye bindin...

Ngày tải lên: 15/02/2014, 01:20

... PRTFDC1 and hypoxanthine. (B) HPRT and hypoxanthine. (C) PRTFDC1 and guanine. (D) HPRT and guanine. Table 2. K m and V max values for Hx and G in the presence of 1 mM PRPP, determined using the DE-81 ... activity of PRTFDC1 towards hypoxanthine and guanine is only 0.26% and 0.09%, respectively, of the activity of HPRT, the role of the PRTFDC1 in purine metaboli...

Ngày tải lên: 15/02/2014, 01:20

... d,l-p-hydroxyphenylhydantoin than HYD Bp does. Homology modeling and substrate docking analyses of HYD Js and HYD Bp were performed, and the results revealed an enlarged substrate binding pocket in HYD Js , ... them in the past. The initial approach to accessing hydantoinases involved screening and iso- lating naturally occurring enzymes possessing hydan- toin-hydrolyzing acti...

Ngày tải lên: 18/02/2014, 08:20

... sub- domain hinge (H1 in Fig. 5) is one of the least con- served motifs and is shorter in eNOS than in nNOS. Swapping the H1 hinge of nNOS into eNOS increased its heme reduction rate and increased ... electron for oxygen activation in place of the flavoprotein, and then redox cycles within NOS; and (d) heme–NO binding is an intrinsic feature of cataly- sis that cons...

Ngày tải lên: 18/02/2014, 11:20

... Comparison of rates and kinetic isotope effects using PEG-modified variants and glycoforms of glucose oxidase: the relationship of modification of the protein envelope to C–H activation and tunneling. ... p-p charge transfer (CT) absorbance and NAD(P)H binding and dissocia- tion can be measured by following the formation of this CT absorbance at, for example, 555 nm whil...

Ngày tải lên: 18/02/2014, 11:20

... protein– flavin complexes and tailor the midpoint potentials in these proteins, as well as many details of the binding and electron transfer to protein and ligand partners, have been revealed. In ... case for R16, K72, and particularly K75 [65,89,100,107]. In addition, K138 and R264 in the NADP + -binding domain of AnFNR are more impor- tant in establishing interactio...

Ngày tải lên: 18/02/2014, 11:20

... FEBS Understanding the binding properties of an unusual metal-binding protein ) a study of bacterial frataxin Chiara Pastore 1 , Marisa Franzese 2 , Filomena Sica 2,3 , Pierandrea Temussi 1,2 and Annalisa ... perturbation of the hydrophobic core of the molecule, which is induced by ion binding at the M4 site. Rearrangement of the Glu19 and Asp23 side chains in uences the ma...

Ngày tải lên: 18/02/2014, 16:20