Báo cáo khoa học: Weak toxin WTX from Naja kaouthia cobra venom interacts with both nicotinic and muscarinic acetylcholine receptors potx

... Authors Journal compilation ª 2009 FEBS 5075 Weak toxin WTX from Naja kaouthia cobra venom interacts with both nicotinic and muscarinic acetylcholine receptors Dmitry Yu. Mordvintsev 1 , Yakov ... CTX, a-cobratoxin; mAChR, muscarinic acetylcholine receptor; MT1, muscarinic toxin 1; MT2, muscarinic toxin 2; MT3, muscarinic toxin 3; MT7, muscarin...

Ngày tải lên: 23/03/2014, 05:22

... mechanistic and crystallographic studies have been carried out with the E. coli and human protein [1]. Epimerase has also been purified and analyzed from the yeast Kluyveromyces fragilis [4–6] and Saccharomyces ... onepimerase andmutarotase activity. Epimerase (10 lg) was incubated with 2 m M 5¢-UMP and 10 m M L -arabinose at room temperature. Aliquots were taken at intervals...

Ngày tải lên: 19/02/2014, 12:20

... phosphorylases from hyperthermophilic Archaea require a CXC motif for stability and folding Giovanna Cacciapuoti, Iolanda Peluso, Francesca Fuccio and Marina Porcelli Department of Biochemistry and Biophysics ... pho- sphorylase II from Sulfolobus solfataricus (SsMTAPII) [10,11] and purine nucleoside phosphorylase from Pyrococccus furiosus (PfPNP) [12], have been isolated and...

Ngày tải lên: 07/03/2014, 00:20

... competition assay was performed with the F-1 fragment and 3 lM of CmtR with no competitor (lane 2), with 50 · non-specific competitor [poly(dI-dC), lane 3], or with 10· (lane 4) and 50· (lane 5) self-competitor; ... to multiple sites in M. tuberculosis DNA and protects an unusually long 90 bp AT-rich sequence from )80 bp to +10 with respect to its own initiation codon. CmtR...

Ngày tải lên: 23/03/2014, 04:21

... min at 4 °C. Resin incubated with RNE-d and fragmented calf thymus DNA was packed in a 3-mL syringe column followed by washing with binding buffer and was eluted with binding buffer containing ... observed with increasing concentrations of alkaline phosphatase from 4 U upwards and the treat- ment of RNE-d with 20 U of enzyme completely abolished DNA binding (lane 2) suggesti...

Ngày tải lên: 23/03/2014, 20:22

... of [eEF1AÆGDPÆtRNA] with PheRS was shown by the gel- shift assay and surface plasmon resonance technique. High stability of both novel ternary and quaternary complexes of eEF1AÆGDP, [eEF1AÆGDPÆtRNA] and [eEF1AÆGDPÆ tRNA Phe ÆPheRS], ... ARS and GDP/GTP exchanging subunits of eEF1. MATERIALS AND METHODS Materials Q-Sepharose, SP-Sepharose and Sephacryl S-400 were purchased fro...

Ngày tải lên: 21/02/2014, 15:20

... W49, N44 ⁄ R53 and F120 ⁄ Y131 of YwlE and BPTP are conserved in PtpB, a lmwPTP from the Gram-negative bacterium Salmonella aureus (F36 and N40) [51], and Etp from E. coli (H42 and K46). Escherichia ... protocol with Monte-Carlo error estimation with respect to the relaxation rates of the tetra- mer (R 1T and R 2T ). Thus, 100 new sets of R 1T and R 2T were generated wi...

Ngày tải lên: 16/03/2014, 02:20

... is semiburied in the binding pocket and 7.75 A ˚ from the T1 copper atom (Fig. 5D). Fig. 1. Sequence alignment of McoP with CotA laccase from Bacillus subtilis (1GSK) and CueO from Escherichia coli (1KV7). ... [35], and McoA from A. aeolicus [4], and they apparently cor- relate with a structural organization of three cupre- doxin-like domains for the ascorbate oxidase, Cot...

Ngày tải lên: 18/02/2014, 04:20

... Data submitted from the kineticswizard populates both databases, and from this, each resource can sub- sequently cross-reference the other, providing a link from kinetic parameters to raw data and vice ... the parameter from the enzyme concentration and increases the usability of the value. To facilitate this further, standard units are specified for all parameters, with subs...

Ngày tải lên: 18/02/2014, 04:20

... sequential phos- phorylation and dephosphorylation kinetics with posi- tive cooperativity favour threshold responses, whereas more processive and or random kinetics without cooperativity result ... essentially random phosphorylation and dephosphorylation. Combinations of random and sequential mechanisms are possible. For example, it is conceivable that phos- phorylation of a protein...

Ngày tải lên: 18/02/2014, 08:20