Báo cáo khoa học: Atomic-resolution structure of reduced cyanobacterial cytochrome c6 with an unusual sequence insertion pot

... FEBS Atomic-resolution structure of reduced cyanobacterial cytochrome c 6 with an unusual sequence insertion Wojciech Bialek 1 , Szymon Krzywda 2 , Mariusz Jaskolski 2,3 and Andrzej Szczepaniak 1 1 ... determination of the crystal structure of cytochrome c6 and comparison with plastocyanin. Structure 3, 1159–1169. 15 Kerfeld CA, Anwar HP, Interrante R, Merc...

Ngày tải lên: 23/03/2014, 05:22

... (dark blue colour) and (B) structure alignment of the fragment 4–7 of the 13 ensemble structures of AII to the fragment 3–6 of the X-ray structure of AII. Fig. 6. Structure of a representative ... small rearrangements of the epitope 4–7 in the solution structure of AII are required by a mean value of 0.76 ± 0.03 A ˚ for structure alignment and  1.27 ± 0.02 A ˚ for...

Ngày tải lên: 20/02/2014, 23:20

... Crystal structure of a staphylokinase variant A model for reduced antigenicity Yuhang Chen 1 , I Gang Song 2 , Fan Jiang 1 , Liang Feng 1 , Xiaoxuan Zhang 1 , Yi Ding 1 , Mark Bartlam 1 , Ao Yang 1 , ... as a cofactor to form a 1 : 1 complex with human plasmin(ogen). The SAK±plasmin (cofactor±enzyme) com- plex, which has proteolytic activity, can form an enzyme± substrate c omplex...

Ngày tải lên: 23/03/2014, 21:21

... solution with four molecules in an asymmetric unit, with a Matthews coefficient probability of 2.61 A 3 ÆDa )1 and a solvent content of 52.9%. Interestingly, molecules A and B (and C and D) formed ... Kruus 2 , Juha Rouvinen 1 and Nina Hakulinen 1 1 Department of Chemistry, University of Eastern Finland, Joensuu, Finland 2 VTT Technical Research Centre of Finland, Espoo, Finlan...

Ngày tải lên: 14/02/2014, 18:20

... was transformed into BL21 (DE3) cells and grown at 37 °C overnight in Luria–Bertani medium containing 30 lgÆmL )1 of kanamycin. Flasks of 400 mL of Luria– Bertani medium (30 lgÆmL )1 kanamycin) ... importin-a and the chloride intracellular chan- nel (CLIC) protein, CLIC4 [2,3]. The structure of a soluble form of CLIC4 shows that it adopts the canonical glutathione S-transferase fo...

Ngày tải lên: 14/02/2014, 19:20

... (Fig. 3A). Three of the ligands, OD2 of Asp165, NE2 of His79 and NE2 of His169, formed an equatorial plane. The other protein ligand, NE2 of His31, bound to the metal, in the company of a water oxygen, ... Suzuki 5 and Yasushi Kawata 3,4 1 National Institute of Advanced Industrial Science and Technology, Ikeda, Osaka, Japan 2 Department of Food Science and Nutrition, Faculty...

Ngày tải lên: 14/02/2014, 22:20

... modeled with a reduced occupancy of 0.6–0.8 to account for the likely effect of radiation damage [33]. In order to determine the structure of the complex of an inactive PhyK mutant with its substrate ... the structure analysis server stan [32]. This model has an R work of 0.180 and R free of 0.206. As suggested by the electron density, the S c atoms of all six cyst...

Ngày tải lên: 16/02/2014, 09:20

... determined the structure of HtA by NMR methods. The structure consists of one a-helix, a helical turn and seven b-strands that form an N-terminal hairpin and an anti-parallel b-sheet, with a characteris- tic ... common tertiary structure [4,13,14]. They fold into an a + b structure with a central five-stranded antiparallel b-sheet and an Keywords cytotoxic protein; NMR;...

Ngày tải lên: 18/02/2014, 08:20

... Human reduced folate carrier: translation of basic biology to cancer etiology and therapy. Cancer Metastasis Rev 26, 111–128. 24 Qiu A, Jansen M, Sakaris A, Min SH, Chattopadhyay S, Tsai E, Sandoval ... activity of the membrane transport sys- tem were not caused by a general loss of intestinal architecture, and hence can be attributed to the specific effect of ethanol ingestion on th...

Ngày tải lên: 18/02/2014, 16:20

... shows excellent stereochemistry, with 98.5% of all residues in the most favored and favored regions of the Ramachandran plot and r.m.s.d. values for bond and angle of 0.005 A ˚ and 1.37 ° as shown in Table ... crystal structure of the complex of this protease with benzamidine. Results and Discussion Overall structure The catalytic domain of DESC1 resembles an oblate elli...

Ngày tải lên: 19/02/2014, 00:20