Báo cáo khoa học: Structural characterization of L-glutamate oxidase from Streptomyces sp X-119-6 pdf

... view of the indi- cated area of the spectrum. (Bottom) An overlay of spectra from the titration of Ca 2+ ⁄ CaM with PhK13 peptide showing in red the spectrum prior to titration and in blue, the spectrum ... over the series of spectra was used to calculate R1 (1 ⁄ T1) and R2 (1 ⁄ T2). The R1 and R2 values were plotted against Fig. 4. (Top) Overlay of two spectra from the titrat...

Ngày tải lên: 19/02/2014, 17:20

... occurrence of PEtn attached to the phosphate group at O-4 of Kdo [11,13,16]. Structure of the core region of the Hep3-glycoforms. In the 1 H NMR spectrum of OS-1 (Fig. 5A), anomeric resonances of HepI–HepIII ... are responsible for most of the steps in the biosynthesis of the OS portion of their LPS molecules. Molecular structural studies of LPS from H. influenzae strai...

Ngày tải lên: 08/03/2014, 02:21

... OLMOL graphic softwares [22]. Fig. 4. NMR characterization of Nogo-24 NH-NH region of a NOESY spectrum of Nogo-24 (mixing time of 250 ms) acquired in an aqueous buffer (50 m M phosphate buffer at pH of 6.5). ... region of a NOESY spectrum (mixing time of 250 ms) of Nogo-40 acquired in a 50 m M phosphate buffer (pH 6.5) at 15 °C. (D) NH-aliphatic region of a NO ESY spectrum o...

Ngày tải lên: 16/03/2014, 16:20

... C ˇ eske ´ Bude ˇjovice; 3 Laboratory of Photosynthesis, Institute of Microbiology, Academy of Sciences of the Czech Republic, Tr ˇ ebon ˇ ; 4 Institute of Physical Biology, University of South Bohemia, C ˇ eske ´ Bude ˇjovice, Czech ... were digitized with a pixel size corresponding to 0.51 nm at the specimen level. Image analyses we re carrie d out using SPIDER software [32]. F...

Ngày tải lên: 16/03/2014, 18:20

... the structure of the LPS O-antigen of F. columnare differs structurally from the LPS O-antigen of the fish pathogen Flavobacterium psychrophilium [21], which is a linear polymer of a trisaccharide ... presence of deoxycholate. Bands were detected using the silver-staining directions of Tsai & Frasch [7]. NMR spectroscopy 1 Hand 13 C NMR spectra were recorded on a Varian Inova...

Ngày tải lên: 17/03/2014, 10:20

... MALDI-TOF MS ⁄ MS analysis for structural elucidation as detailed in the Materials and Methods. MALDI-TOF spectra of peak 1 (0.2 M-eluted) from the SFC group (A) and its respective MALDI-TOF MS ... 3000 3500 4000 4500 5000 5500 m/z Fig. 9. MALDI-TOF spectra of peak 2 (0.3 M-eluted) from the FFD-500 group (A) and its respective MALDI-TOF MS ⁄ MS spectra (B). All other details are as...

Ngày tải lên: 22/03/2014, 17:20

... 2009) doi:10.1111/j.1742-4658.2009.07103.x l-Glutamate oxidase (LGOX) from Streptomyces sp. X-119-6, which cata- lyzes the oxidative deamination of l-glutamate, has attracted increasing attention as a component of amperometric l-glutamate ... University of Tsukuba, Japan Keywords L-amino acid oxidase; L-glutamate oxidase; Streptomyces; substrate specificity; X-ray cryst...

Ngày tải lên: 23/03/2014, 05:22

... possibility of functional dissection of the dynamin protein into specific domains suggests that a detailed characterization of the intrinsic structural and dynamic characteristics of the individual ... interior of the A B Fig. 2. Histogram of distribution of hydrodynamic radii obtained from ‘regularization analysis’ of data from dynamic light scattering experiments. (A)...

Ngày tải lên: 23/03/2014, 11:20

... Yusof 1 , Erning Gao 1 , Hong-Li Yan 2 and Andreas Hofmann 1 1 Institute of Structural & Molecular Biology, School of Biological Sciences, The University of Edinburgh, UK 2 Department of ... interactions; protein– membrane interactions Correspondence A. Hofmann, Institute of Structural & Molecular Biology, School of Biological Sciences, The University of Edinburgh, T...

Ngày tải lên: 07/03/2014, 12:20

... which has been inter- preted as a phosphate ion originating from the crystallization buffer. The position of the phosphate corresponds to the c-phosphate of the dTTP molecule bound as feedback ... nitrogen from His58, both situated on the P-b-hairpin, also make hydrogen bonds with the phosphates (Fig. 2B). The b-phosphate of dTTP as phosphate donor is very well aligned with the c-ph...

Ngày tải lên: 23/03/2014, 09:21