Báo cáo khoa học: The ferredoxin-NADP+ reductase ⁄ferredoxin electron transfer system of Plasmodium falciparum docx

... optimizing the K d values. Lower panel: plot of the calculated K d values of the PfFNR–PfFd complex as a func- tion of the ionic strength of the medium. E. Balconi et al. Plasmodium falciparum ... consisting of the partial unwinding of the aF helix (Fig. 6A) and other minor conformational changes. In the ligand-bound form of PfFNR, the formation of a AB F...

Ngày tải lên: 23/03/2014, 05:22

... suggesting the existence of a third inhibitory site. Univalent cations also influence the activity of enolases. Most of the enolases are inhibited by Na + ,whereasthe effect of K + depends on the source ... xamine whether modification of Cys157 and other P. falciparum- specific residues in the vicinity of the active site leads to irreversible inactivation of Pfen. Com...

Ngày tải lên: 16/03/2014, 18:20

... the same role in electron transfer in nitrate reductase. The [4Fe-4S] cluster of high-potential would therefore be the indispensable relay for the transfer of electrons when menadiol is the electron ... that the transfer of electrons up to the molyb- denum cofactor in nitrate reductase follows two distinct pathways according to the nature of the electro...

Ngày tải lên: 07/03/2014, 15:20

... proteins; M pep , the number of unique matched peptides; U pep , the number of unmatched peptides in the MALDI-TOF experiments. The function of new proteins is based on the GH family of the catalytic ... synergies of 1.2 and 1.3, respec- tively, and a synergy of 1.2 was also obtained with the combination F3 ⁄ F5 ⁄ F6. On straw, the activity of the control sampl...

Ngày tải lên: 18/02/2014, 08:20

... using the program SEDFIT, and the data were then directly fitted using the finite element solution of the Lamm equation in SEDFIT [18] to give values of the sedimentation coefficient in the buffer of ... rec- ognize the lipoyl-domain of the Thermoplasma E2 enzyme, the target lysine of which is flanked by D and V residues, as it is in the E2 protein of the E. coli...

Ngày tải lên: 16/03/2014, 05:20

... investigate the in vivo functions of each of the A, B and C types of plant multi-RPA systems. In vivo roles of the multi-RPA system If the multi-RPA system is unique in plants, some of the in vivo ... only one copy of the gene for the RPA70kDa subunit in their genome? Furthermore, is only the larg- est subunit of the RPA complex duplicated in plant, and wha...

Ngày tải lên: 23/03/2014, 06:20

... domain. In the case of helicases, dimerization is of clear benefit for the processivity of the helicase reaction, such that alternating subunits can be engaged in unwinding the DNA duplex or tethering the ... show that dimerization occurs through the central ATPase domain of the pro- tein. These results have implications for the mechanism of action of CSB, and sugge...

Ngày tải lên: 23/03/2014, 15:20

... the EGF domain and six in the CFC domain. To test whether the CFC domain could retain its function independent of the EGF domain, we generated a soluble form of the CFC domain, comprised of the ... signi- ficance of the CFC domain. In the present work, we have solved the disulfide structure of the CFC domain and have conducted biochemical studies that detail the...

Ngày tải lên: 23/03/2014, 21:20

... modules. The pathways for the individual carbohydrates, including the description of protein synthesis, are connected to the glycolysis module. The PTS reactions, the synthesis of cAMP and the activation ... popular [21]. One advantage of the method of modular modeling is that the granularity of the submodels can easily be adjusted to the objective of the m...

Ngày tải lên: 18/02/2014, 13:20

... .5. ity of the transition distributions, are stronger than the effects of β, which determines the probability of the output distributions. The optimal value of .003 for α reflects the fact that the ... of 74.5%, and is closer to the 90.1% accuracy of CRF/CE on the same data set using oracle parameter selection. The effects of α, which determines the probabil...

Ngày tải lên: 20/02/2014, 12:20