Báo cáo khoa học: The pivotal regulator GlnB of Escherichia coli is engaged in subtle and context-dependent control potx

... implications and the mecha- nistic basis for this conditional redundancy of GlnB (and GlnK) are discussed. Results Modulation of the GlnB concentration in vivo and the levels of GlnB and GlnK In order ... with the puzzle of a func- tional explanation for the existence of GlnB: why should control by P II * be absent altogether, and what then is the...

Ngày tải lên: 23/03/2014, 04:21

... polypeptide is a nucleic acid binding protein involved in the synthesis of many polypeptides. Hfq particularly affects the translation and the stability of several RNAs. In an earlier study, the use of ... transform infra-red. On the basis of these results, we propose that the C-terminal domain could protect the interface between the subunits and stabilize th...

Ngày tải lên: 19/02/2014, 12:20

... glycine, histidine, isoleucine, leucine, phenyl- alanine, proline, serine, threonine, tyrosine, and valine for [U- 13 C]glucose experiments and aspartate, isoleucine, leu- cine, phenylalanine, ... growth and glucose uptake rates. While the flux profiles were similar in the three wild-type strains with small differences in the fractions of serine originating from glycine and...

Ngày tải lên: 20/02/2014, 23:20

... 3A,B). The presence of Ca 2+ increased the thermal stability of PhyH and PhyH-DII. After incubation at 60 °C for 30 min, both enzymes retained > 70% of their initial activity in the presence of ... Ala208 and Gly234). Some of the res- idues involved in phosphate and calcium binding are conserved in the PhyH-DII sequence, whereas only one phosphate-binding re...

Ngày tải lên: 14/02/2014, 15:20

... glutaredoxin, might be required for reduction of the mixed disulfide. In this hypothesis, glutaredoxins would be involved in a signaling pathway contributing to the redox regulation of the Calvin cycle ... down-regulation of the Calvin cycle under stress would allow redistribution of the reducing power in the chloroplast by decreasing NADPH consumption and thereby i...

Ngày tải lên: 19/02/2014, 05:20

... characteristics of this mapping. The results indicate that human naming performance is influenced by the frequency of graphemic units in the language and by the predictability of their mapping ... resolution of these issues. In this paper, we present a descriptive analysis of the grapheme-phoneme mapping system of the French orthography, and we further...

Ngày tải lên: 20/02/2014, 19:20

... determine the N-terminal amino acid sequence of the larger subunit using a number of different preparations of the protein. The gene encoding the smaller subunit (fdh1B)wasidentified in the unfinished ... shown in blue. Amino acids coordinating the two molybdopterin guanine dinucletoide cofactors of Fdh H of E. coli (MGD 801 and MGD 802 )that were found to be inv...

Ngày tải lên: 20/02/2014, 23:20

... the PolC N-terminus is involved in mediating the PolC-s interaction and possi- bly also in binding DNA. Introduction Genome replication in bacteria is carried out by the multicomponent protein ... and PolC at the domain level. These include the proofread- ing 3¢–5¢ exonuclease domain, oligonucleotide ⁄ oligo- saccharide-binding (OB) domain and the additional N- an...

Ngày tải lên: 05/03/2014, 23:20

... in many protein folding studies [2,3]. The 123 residue chain of a-LA is organized into a discontinuous a-helical domain composed of residues 1–39 and 81–123, and a small b-domain comprising the ... complex. This view is in line with the fact that all variants of a-LA of human, bovine, equine, porcine and caprine origin, as well as recombi- nant mutants of a-LA de...

Ngày tải lên: 06/03/2014, 09:22

... chains. Another interesting outlook is the identification of a putative cytosolic binding partner of the ISP, e.g. by using chemical cross-linking approaches and MS, giving interesting insights into ... effects. The [2Fe)2S] cluster of the Rieske protein is inserted in the cytoplasm As cytosolic cofactor insertion is one of the hallmarks of Tat translocation, t...

Ngày tải lên: 07/03/2014, 11:20