Báo cáo khoa học: Hyperefficient PrPSc amplification of mouse-adapted BSE and scrapie strain by protein misfolding cyclic amplification technique pptx

... Hyperefficient PrP Sc amplification of mouse-adapted BSE and scrapie strain by protein misfolding cyclic amplification technique Aiko Fujihara, Ryuichiro Atarashi, ... PrP Sc amplification using the PMCA tech- nique. We chose mouse-adapted BSE (mBSE) and mouse-adapted scrapie, Chandler strain, as animal models for TSEs. Here, we describe a hyperefficient...

Ngày tải lên: 23/03/2014, 04:21

... weight of a path is calculated as the product of the weights of its edges, and that the weight of a (not necessarily finite) set T of paths is calculated as the sum of the weights of the paths of ... on-the-fly and bucket brigade algorithms, and also discussed the possibility of embed-compose-project and of ine composition strategies to application of cascades of...

Ngày tải lên: 17/03/2014, 00:20

... cross-linking and mass spectro- metry for mapping three-dimensional structures of proteins and protein complexes. J Mass Spectrom 38, 1225–1237. 7 Wong SS (1991) Chemistry of Protein Conjugation and Cross-Linking. ... structures, chemical modifications and cross-linking in protein mixtures, complexes, and assemblies. Proteins A single protein, a list of proteins from a m...

Ngày tải lên: 07/03/2014, 12:20

... from the hearts of control (C) and MCK (M) mice at 3, 5, 7 and 10 weeks of age were analyzed by immunoblotting with antibodies against Lon and ClpP. Immunolabeled protein bands of interest were ... subunit of complex I and the flavoprotein (SDHA) of complex II. A representative result of three independent experiments is shown. (B) Immunolabeled protein bands of inte...

Ngày tải lên: 23/03/2014, 06:20

... assay One thousand cells of HeLaTR and FRTL were cultured in 100 lL of the medium in the presence or absence of T3 and Z-VAD-FMK at 37 °C for 48 h. At the end of the culture, 20 lL of CellTiter ... caspases by T3. The biological significance of the modulation of PSG7, fmfc and BMP-6 expression by T3 also awaits additional studies. Up-regulation of 4-1BB and the subs...

Ngày tải lên: 23/03/2014, 18:20

... control ODN (con) and the dead cells were counted after 16, 24 and 48 h of culture using trypan blue staining. (C) Cells were treated with 0.5, 1 and 2 lg of hpST3dODN and 1 and 2 lg of control ODN ... induces cell death of SW480 cells. (A) SW480 cells were incubated in the absence of IFN-c or with 10, 100 and 200 ngÆmL )1 for 4, 24 and 48 h of incubation and cell...

Ngày tải lên: 18/02/2014, 08:20

... entropic cost of ligation is rather low and can be compensated for by the favourable reaction enthalpy of formation of the 5¢,3¢-phospho- diester bond via ring opening of the 2¢,3¢ -cyclic phos- phate ... structure of the reverse-joined ribozyme unit for twin ribozyme design consists of two domains joined by a single-stranded linker of eight adenosine residues and binds...

Ngày tải lên: 20/02/2014, 01:20

... have a reservoir of size 5, containing three values of −1, and two values of 1, then the exchangeabil- ity of the elements means the reservoir is fully characterized by knowing k, and that there ... various values of s and u. Algorithm 1 RESERVOIRUPDATE(n, k, m, σ, s) Parameters: n : size of stream so far k : size of reservoir, also maximum value of s m : magnitude of...

Ngày tải lên: 20/02/2014, 04:20

... with a strand of siRNA, and the other siRNA strand was subsequently annealed to the aptamer-conjugated strand. These aptamer–siRNA conjugates were able to access HIV- infected cells and silence ... 4827 MINIREVIEW Efficient and targeted delivery of siRNA in vivo Min Suk Shim 1 and Young Jik Kwon 1,2,3 1 Department of Chemical Engineering and Materials Science, University of C...

Ngày tải lên: 06/03/2014, 01:23

... sibling grandchild tri-siblinggrand-sibling Specifically, Sections 4.1, 4.2, and 4.3 describe parsers that, respectively, factor trees into grand- child parts, grand-sibling parts, and a mixture of grand-sibling ... parts—combinations of sib- ling parts and grandchild parts. Specifically, a grand-sibling is a 4-tuple of indices (g, h, m, s) where (h, m, s) is a sibling part and (g...

Ngày tải lên: 07/03/2014, 22:20